- Large-scale preparation of the oligosaccharide phosphate fraction of Pichia holstii NRRL Y-2448 phosphomannan for use in the manufacture of PI-88
-
Mild acid-catalysed hydrolysis of the extracellular phosphomannan of the yeast Pichia holstii NRRL Y-2448 produces a high-molecular-weight phosphomannan core, a low-molecular-weight oligosaccharide phosphate fraction, and a neutral oligosaccharide fraction. A method was developed for the large-scale preparation of the oligosaccharide phosphate fraction, consisting predominantly of the pentasaccharide phosphate, 6-O-PO3H2-α-D-Man-(1 → 3)-α-D-man-(1 → 3)-α-D-man-(1 → 3)-α-D-man-(1 → 2)-D-man, for use in the manufacture of the promising new anti-cancer agent, PI-88. Further insights were also gained into the structure of the phosphomannan by HPLC analysis of the time course of the hydrolysis reaction.
- Ferro, Vito,Fewings, Kym,Palermo, Maria C.,Li, Caiping
-
-
Read Online
- Enzymatic synthesis of mannobioses and mannotrioses by reverse hydrolysis using α-mannosidase from Aspergillus niger
-
Various manno-oligosaccharides including α-D-man-(12)-D-man and α-D-man-(12)-α-D-man-(12)-D-man were formed when a highly concentrated mannose solution was incubated in the presence of α-mannosidase from Aspergillus niger. α-D-Man-(12)-D-man and α-D-man-(12)-α-D-man-(12)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column.In addition to the above oligosaccharides, α-D-man-(13)-D-man, α-D-man-(16)-D-man, and α-D-man-(12)-α-D-man-(16)-D-man were also isolated. Keywords: Mannobioses; Mannotrioses; α-Mannosidase; Aspergillus niger
- Ajisaka, Katsumi,Matsuo, Ichiro,Isomura, Megumi,Fujimoto, Hiroshi,Shirakabe, Mayumi,Okawa, Mitsuyo
-
-
Read Online
- Glycosidase-catalysed synthesis of mannobioses by the reverse hydrolysis activity of α-mannosidase: Partial purification of α-mannosidases from almond meal, limpets and Aspergillus niger
-
Two disaccharides, α-D-Manp-(1→2)-D-Manp 6 and α-D-Manp-(1→3)-D-Manp 7, were synthesised from mannose using the reverse hydrolysis activity of the partially purified α-mannosidases from almond (Prunus amygdalus) meal and limpets (Patella vulgata). Both di
- Singh, Suddham,Scigelova, Michaela,Crout, David H. G.
-
-
Read Online
- A novel two-step synthesis of α-linked mannobioses based on an acid-assisted reverse hydrolysis reaction
-
Instead of an enzyme-assisted reverse hydrolysis reaction for the synthesis of manno-oligosaccharides, we propose here a versatile new approach. By Fischer type glycosylation, a d-mannose solution of extremely high concentration (approximately 83% (w/w)) was incubated at 60 °C for 65 h in 0.5 M HCl. After dilution and neutralization, the small amount of formed β-linked oligosaccharides was hydrolyzed by β-mannosidase. The yields of α-d-Manp-(1→2)-d-Manp (7.9%), α-d-Manp-(1→3)-d-Manp (7.9%), and α-d-Manp-(1→6)-d-Manp (29.1%) isolated by an activated carbon column chromatography were almost identical to those of the enzymatic reaction, but the yield of α-d-Manp-(1→3)-d-Manp increased enormously by the present method.
- Ajisaka, Katsumi,Yagura, Misato,Miyazaki, Tatsuo
-
-
Read Online
- Multivalent glyconanoparticles with enhanced affinity to the anti-viral lectin Cyanovirin-N
-
Low-mannose (LM) structures were coupled to gold nanoparticles (Au NPs) to amplify the affinity of LMs with Cyanovirin-N (CV-N) lectins and to study the structures of CV-N variants CVNQ50C and CVNMutDB. The Royal Society of Chemistry
- Wang, Xin,Matei, Elena,Deng, Lingquan,Ramstroem, Olof,Gronenborn, Angela M.,Yan, Mingdi
-
supporting information; experimental part
p. 8620 - 8622
(2011/09/19)
-
- Synthesis and heparanase inhibitory activity of sulfated mannooligosaccharides related to the antiangiogenic agent PI-88
-
A stepwise synthetic route to the mannooligosaccharides from the neutral fraction of Pichia holstii phosphomannan hydrolysate, including a tetrasaccharylamine component, was developed using only two or three readily available d-mannose building blocks. Th
- Fairweather, Jon K.,Hammond, Edward,Johnstone, Ken D.,Ferro, Vito
-
p. 699 - 709
(2008/09/17)
-