Enzymatic synthesis of mannobioses and mannotrioses by reverse hydrolysis using α-mannosidase from Aspergillus niger

Article abstract of DOI:10.1016/0008-6215(95)00015-L

Various manno-oligosaccharides including α-D-man-(1<*>2)-D-man and α-D-man-(1<*>2)-α-D-man-(1<*>2)-D-man were formed when a highly concentrated mannose solution was incubated in the presence of α-mannosidase from Aspergillus niger. α-D-Man-(1<*>2)-D-man and α-D-man-(1<*>2)-α-D-man-(1<*>2)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column.In addition to the above oligosaccharides, α-D-man-(1<*>3)-D-man, α-D-man-(1<*>6)-D-man, and α-D-man-(1<*>2)-α-D-man-(1<*>6)-D-man were also isolated. Keywords: Mannobioses; Mannotrioses; α-Mannosidase; Aspergillus niger