203435-53-4Relevant articles and documents
Chemoproteomic Profiling of Phosphoaspartate Modifications in Prokaryotes
Chang, Jae Won,Montgomery, Jeffrey E.,Lee, Gihoon,Moellering, Raymond E.
, p. 15712 - 15716 (2018)
Phosphorylation at aspartic acid residues represents an abundant and critical post-translational modification (PTM) in prokaryotes. In contrast to most characterized PTMs, such as phosphorylation at serine or threonine, the phosphoaspartate moiety is intr
Intracellular Delivery of Native Proteins Facilitated by Cell-Penetrating Poly(disulfide)s
Qian, Linghui,Fu, Jiaqi,Yuan, Peiyan,Du, Shubo,Huang, Wei,Li, Lin,Yao, Shao Q.
, p. 1532 - 1536 (2018)
Intracellular delivery of therapeutic proteins is highly challenging and in most cases requires chemical or genetic modifications. Herein, two complementary approaches for endocytosis-independent delivery of proteins to live mammalian cells are reported. By using either a “glycan” tag naturally derived from glycosylated proteins or a “traceless” tag that could reversibly label native lysines on non-glycosylated proteins, followed by bioorthogonal conjugation with cell-penetrating poly(disulfide)s (CPDs), we achieved intracellular delivery of proteins (including antibodies and enzymes) which, upon spontaneous degradation of CPDs, led to successful release of their “native” functional forms with immediate bioavailability.
Design and Synthesis of an RGD Peptidomimetic-Paclitaxel Conjugate Targeting α v β 3 Integrin for Tumour-Directed Drug Delivery
Piras, Monica,Andriu, Alexandra,Testa, Andrea,Wienecke, Paul,Fleming, Ian N.,Zanda, Matteo
, p. 2769 - 2776 (2017)
A 1,2,3-triazole-based RGD peptidomimetic having nanomolar affinity for α v β 3 integrin was conjugated to the potent antimitotic paclitaxel via an oxime heterobifunctional linker. The resulting construct maintained nanomolar binding
Aldehyde-mediated bioconjugation: Via in situ generated ylides
Parmar, Sangeeta,Pawar, Sharad P.,Iyer, Ramkumar,Kalia, Dimpy
supporting information, p. 14926 - 14929 (2019/12/24)
A technically simple approach for rapid, high-yielding and site-selective bioconjugation has been developed for both in vitro and cellular applications. This method involves the generation of maleimido-phosphonium ylides via 4-nitrophenol catalysis under physiological conditions followed by their Wittig reactions with aldehyde-appended biomolecules.
Diverse organo-peptide macrocycles via a fast and catalyst-free oxime/intein-mediated dual ligation
Satyanarayana, Maragani,Vitali, Francesca,Frost, John R.,Fasan, Rudi
supporting information; experimental part, p. 1461 - 1463 (2012/03/26)
Macrocyclic Organo-Peptide Hybrids (MOrPHs) can be prepared from genetically encoded polypeptides via a chemoselective and catalyst-free reaction between a trifunctional oxyamino/amino-thiol synthetic precursor and an intein-fusion protein incorporating a bioorthogonal keto group.
C-Terminal Attachment of Two Chemical Groups to Peptides
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, (2010/05/13)
The present invention relates to a method for C-terminal attachment of two property-modifying groups to a peptide.
Insulin Derivatives Conjugated with Structurally Well Defined Branched Polymers
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, (2009/07/02)
Insulin conjugated with structurally well defined, bifurcated and trifurcated polymers can be use by pulmonary delivery for systemic absorption through the lungs to reduce or eliminate the need for administering other insulins by injection.
C-Terminally Pegylated Growth Hormones
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, (2009/05/29)
Conjugated growth hormones of the structure (I) are provided together with methods for manufacturing said conjugates. The conjugates are useful in therapy.
N-TERMINAL PEGYLATED PROLACTIN RECEPTOR MOLECULES
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Page/Page column 42-43, (2008/12/05)
The invention is concerned with N-terminally pegylated polypeptides capable of binding to the prolactin receptor. Such polypeptides may for instance be N-terminally pegylated antagonists of the prolactin receptor, such as for instance prolactin variants.
C-Terminally PEGylated hGH-derivatives
Peschke, Bernd,Zundel, Magali,Bak, Sonja,Clausen, Trine R.,Blume, Niels,Pedersen, Anja,Zaragoza, Florencio,Madsen, Kjeld
, p. 4382 - 4395 (2008/03/13)
A two-step strategy was used for the preparation of C-terminally PEGylated hGH-derivatives. In a first step a CPY-catalyzed transpeptidation was performed on hGH-Leu-Ala, introducing reaction handles, which were used in the second step for the ligation of