- Enzymatic synthesis and RNA interference of nucleosides incorporating stable isotopes into a base moiety
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Thymidine phosphorylase was used to catalyze the conversion of thymidine (or methyluridine) and uracil incorporating stable isotopes to deoxyuridine (or uridine) with the uracil base incorporating the stable isotope. These base-exchange reactions proceede
- Hatano, Akihiko,Shiraishi, Mitsuya,Terado, Nanae,Tanabe, Atsuhiro,Fukuda, Kenji
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- Synthetic method of double-labeled uridine-(C,N2)
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The invention relates to a synthetic method of double-labeled uridine-(C,N2). The method comprises the following steps: carrying out a cyclization reaction on double-labeled urea-(C,N2) and propiolic acid in a solvent anhydrous benzene to obtain double-labeled uracil-(C,N2); and mixing the double-labeled uracil-(C,N2) with 1-acetoxy-2,3,5-tribenzoyloxy-beta-D-ribofuranose in a solvent acetonitrile in Ar2 atmosphere, adding hexamethyldisilazane and trimethylsilyl trifluoromethanesulfonate, post-processing above materials to obtain an intermediate, and hydrolyzing the intermediate with ammonia water to obtain the double-labeled uridine-(C,N2). Compared with the prior art, the method disclosed in the invention has the advantages of simple process, high yield, no decrease of the isotope abundance, and suitableness for laboratory production of the double-labeled uridine-(C,N2).
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Paragraph 0013; 0023; 0027-0028; 0032-0033; 0037-0038
(2017/08/27)
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