- Preparation method of N alpha- fluorenylmethoxycarbonyl-N Epsilon-7-methoxycoumarin-4-acetyl-lysine/ornithine
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The invention relates to a preparation method of N alpha-fluorenylmethoxycarbonyl-N-Epsilon-7-methoxycoumarin-4-acetyl-lysine/ornithine and mainly aims to solve the problems that a current method is complicated in treatment, lower in yield and not environment-friendly. According to the technical scheme, the preparation method of N alpha-fluorenylmethoxycarbonyl-N-Epsilon-7-methoxycoumarin-4-acetyl-lysine/ornithine comprises steps as follows: N alpha-fluorenylmethoxycarbonyl-N Epsilon-7-methoxycoumarin-4-acetyl-lysine/ornithine tert-butyl ester is produced from N alpha-fluorenylmethoxycarbonyl-lysine/ornithine tert-butyl ester and 7-methoxycoumarin-4-acetic acid through condensation under catalysis of a condensing agent, tert-butyl ester is removed from N alpha-fluorenylmethoxycarbonyl-N Epsilon-7-methoxycoumarin-4-acetyl-lysine/ornithine tert-butyl ester by use of trifluoroacetic acid, and a product, namely, N alpha-fluorenylmethoxycarbonyl-N-Epsilon-7-methoxycoumarin-4-acetyl-lysine/ornithine is obtained. The product is mainly used as a fluorescent probe or a marker.
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Paragraph 0013
(2017/08/29)
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- Fluorescent labeling of peptides on solid phase
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N α-Fmoc-Nε-[(7-methoxycoumarin-4-yl) acetyl]-l-lysine (Nα-Fmoc-l-Lys(Mca)-OH) 3 is conveniently prepared by benzotriazole methodology (52% over two steps). N-Acylbenzotriazoles Mca-Bt 2, Nα-Fmoc-l-Lys(Mca)-Bt 4
- Katritzky, Alan R.,Yoshioka, Megumi,Narindoshvili, Tamari,Chung, Alfred,Johnson, Jodie V.
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experimental part
p. 4582 - 4586
(2009/03/12)
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