5625-37-6

Basic information

• Product Name: PIPES
• Synonyms: PIPES Piperazine-1,4-bisethanesulfonic acid;PIPES,98%;PIPES Vetec(TM) reagent grade, >=99%;ABTS DIAMMONIUM SALT ULTRA PURE GRADE;MOLYBDENE STAND;MOLYBDENUM STAND;PIPES BIOXTRA, FOR MOLECULAR BIOLOGY;PIPES, BIOPERFORMANCE CERTIFIED&
• CAS NO: 5625-37-6
• Molecular Formula: C₈H₁₈N₂O₆S₂
• Molecular Weight: 302.37
• EINECS: 227-057-6
• Product Categories: Biochemistry;Good's Buffers;Buffer
• Mol File: 5625-37-6.mol

Chemical Properties

• Melting Point: >300 °C(lit.)
• Appearance: White/Micro-Crystalline Powder
• Density: 1.4983 (rough estimate)
• Refractive Index: 1.6300 (estimate)
• Storage Temp.: Store at RT.
• Solubility: 0.1 M NaOH: 0.25 g/mL, clear, colorless
• PKA: 6.8(at 25℃)
• Water Solubility: Soluble in water.
• Merck: 14,7479
• BRN: 817713
• CAS DataBase Reference: PIPES(CAS DataBase Reference)
• NIST Chemistry Reference: PIPES(5625-37-6)
• EPA Substance Registry System: PIPES(5625-37-6)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 22-24/25-37/39-26
• WGK Germany: 3
• TSCA: Yes
• Hazardous Substances Data: 5625-37-6(Hazardous Substances Data)

Usage

Uses

1. Used in Biochemistry:
PIPES is used as a buffering agent with a pKa near physiological pH, making it ideal for cell culture work and other biological experiments using an intracellular solution.
2. Used in Cell Culture:
PIPES is employed in cell culture applications, such as the engineering of a thermostable mutant membrane protein in Escherichia coli, due to its pKa value near physiological pH.
3. Used in Glutaraldehyde Fixation:
PIPES is used to buffer the glutaraldehyde fixative in the fixation of plant and animal tissue samples, minimizing lipid loss and artifacts.
4. Used in Protein Crystallization:
PIPES has been utilized in protein crystallization, a technique used to determine the three-dimensional structure of proteins.
5. Used in Gel Electrophoresis:
PIPES serves as a running buffer in gel electrophoresis, a method used to separate proteins or nucleic acids based on their size and charge.
6. Used in Isoelectric Focusing and Chromatography:
PIPES is used as an eluent in isoelectric focusing and chromatography, techniques used to separate proteins based on their isoelectric points.
7. Used in Enzyme Activity Retention:
PIPES is used as a buffer to retain alkaline phosphatase activity in rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde.
8. Used in Fluorescence Microscopy and Electron Microscopy:
PIPES is used in the fixation of fungal zoospores for optimal results in fluorescence microscopy and electron microscopy when combined with glutaraldehyde and formaldehyde.
9. Used in Buffers for In Vitro Studies:
PIPES is recommended for use in buffers for the in vitro study of caspases 3, 6, 7, and 8, which are important enzymes involved in the process of apoptosis.
10. Used in Non-Metal Ion Complexing Buffer Applications:
PIPES is used as a non-metal ion complexing buffer in protein assays, such as the bicinchoninic acid (BCA) assay.
11. Used in RNA and Protein Studies:
PIPES has been reported for use in protocols involving the separation of glyoxylated RNA in agarose gels, nuclease S1 mapping of RNA, ribonuclease protection assay protocols, and the reconstitution of dissociated tubulin α and β subunits after their resolution on immunoadsorbent gels.

Flammability and Explosibility

Notclassified

Purification Methods

Purify PIPES from boiling water (maximum solubility is about 1g/L) or as described for ADA [N-(2-acetamido)iminodiacetic acid above]. [Good et al. Biochemistry 5 469 1966, Beilstein 23/12 V 380.]

InChI:InChI=1/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

Synthetic route

piperazine (110-85-0) + Na-salt of 2-chloroethane-1-sulfonic acid (15484-44-3) → 2,2'-piperazine-1,4-diyl-bis-ethanesulfonic acid (5625-37-6)

Conditions	Yield
With sodium hydroxide In water for 3h; pH=9 - 9.5;	86.3%

2,2'-piperazine-1,4-diyl-bis-ethanesulfonic acid (5625-37-6) → Glyoxal (131543-46-9) + 2,2'-Ethylendiimido-diethansulfonsaeure (25127-57-5)

Conditions	Yield
With (batho)2CuII (batho = 2,9-dimethyl-4,7-diphenyl-1,10-phenanthrolinedisulfonate); water at 25℃; for 24h; Rate constant; pH 8;

hexamethylenetetramine (100-97-0) + silver(I) acetate (563-63-3) + 2,2'-piperazine-1,4-diyl-bis-ethanesulfonic acid (5625-37-6) → 2Ag(1+)*(CH2)4N2(CH2CH2SO3)2(2-)*2N4(CH2)6*14H2O=[Ag2(C8H16N2O6S2)(C6H12N4)2(H2O)2]*12H2O

Conditions	Yield
In ammonia; water aq. NH3; acid and amine added to aq. soln. of AgCH3COO; stirred for 15 min; ppt. dissolved by dropwise addn. of aq. NH3; soln. evapd. for 2 d at room temp.;

ammonium metavanadate + cobalt(II) chloride hexahydrate + 2,2'-piperazine-1,4-diyl-bis-ethanesulfonic acid (5625-37-6) → 2C8H18N2O6S2*3Co(2+)*18H2O*10V(5+)*28O(2-)

Conditions	Yield
With sodium hydroxide In methanol; water for 1.5h; pH=6;

Upstream product

• 110-85-0 piperazine 
• 15484-44-3 Na-salt of 2-chloroethane-1-sulfonic acid 

Relevant articles and documents

Preparation method of piperazine ethanesulfonic acid derivative (by machine translation)

The reaction is carried out in such a manner that the reaction proceeds relatively, more thoroughly by reducing the side, reaction of the reaction system, in a manner such that the reaction proceeds relatively more thoroughly from, the system to 2 - a certain extent 2 - by reducing the reaction proceeds, to relatively more thoroughly; NaOH, NaCl, and. (by machine translation)

Stabilizing labeled antibody using amino acids

The present invention relates to a method for stabilizing a labeled antibody in a solution, in which the labeled antibody is stabilized by allowing the labeled antibody to be present together with at least one of amino acid and a derivative thereof in the solution.