72-57-1 Usage
Uses
Used in Biological Staining:
Direct Blue 14 is used as a biological stain for various applications, including staining tissues and cells for microscopic examination.
Used in Cell Viability Assessment:
Direct Blue 14 is used as a cell viability indicator in the Trypan Blue exclusion assay. It stains dead cells with permeable membranes blue, while living cells with intact membranes exclude the dye. This allows for the visual determination of living versus dead cells, making it an easy method for assessing cell viability in cultured cells and other samples such as rat abdominal organ slices and islet cell clusters from caprine pancreatic samples.
Preparation
3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid(2 Moore) coupled.
Reactivity Profile
Direct Blue 14 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 14 is incompatible with strong oxidizing agents and strong acids.
Health Hazard
ACUTE/CHRONIC HAZARDS: Direct Blue 14 may cause irritation and may be absorbed through the skin. It is a positive animal carcinogen. When heated to decomposition Direct Blue 14 emits very toxic fumes of nitrogen oxides, sulfur oxides, carbon monoxide and carbon dioxide.
Fire Hazard
Flash point data for Direct Blue 14 are not available; however, Direct Blue 14 is probably combustible.
Biochem/physiol Actions
Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.
Safety Profile
Suspected carcinogen
with experimental carcinogenic,
neoplastigenic, and tumorigenic data. Poison
by intraperitoneal, intravenous, and
subcutaneous routes. Experimental
teratogenic and reproductive effects.
Mutation data reported. When heated to
decomposition it emits very toxic fumes of
NOx NazO, and SOx
Potential Exposure
Used in dyeing textiles; leather and
paper; as a biological stain.
Standard
Acid Resistance
Fading
Stain
ISO
4-5
AATCC
5
Incompatibilities
Dust may form explosive mixture with
air. Trypan Blue is incompatible with strong oxidizing
agents. Incompatible with oxidizers (chlorates, nitrates,
peroxides, permanganates, perchlorates, chlorine, bromine,
fluorine, etc.); contact may cause fires or explosions. Keep
away from alkaline materials, strong bases, strong acids,
oxoacids, epoxides. Azo compounds can detonate. This
applies in particular to organic azides that have been sensitized
by the addition of metal salts or strong acids. Toxic
gases are formed by mixing materials of this class with
acids, aldehydes, amides, carbamates, cyanides, inorganic
fluorides, halogenated organics, isocyanates, ketones,
metals, nitrides, peroxides, phenols, epoxides, acylhalides,
and strong oxidizing or reducing agents. Flammable gases
are formed by mixing materials in this group with alkali
metals. Explosive combinations can occur with strong oxidizing
agents, metal salts, peroxides, and sulfides. Trypan
Blue is sensitive to prolonged exposure to heat.
Check Digit Verification of cas no
The CAS Registry Mumber 72-57-1 includes 5 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 2 digits, 7 and 2 respectively; the second part has 2 digits, 5 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 72-57:
(4*7)+(3*2)+(2*5)+(1*7)=51
51 % 10 = 1
So 72-57-1 is a valid CAS Registry Number.
InChI:InChI=1/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,37-38H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4/b39-31+,40-32+;;;;
