- NUCLEOSIDES AND NUCLEOTIDES. PART 27. SOLID PHASE SYNTHESIS OF A TRIDECANUCLEOSIDE DODECAPHOSPHATE CONTAINING THE UNNATURAL BASE 4-AMINO-2(1H)-PYRIDINONE AND OF AN OCTANUCLEOSIDE HEPTAPHOSPHATE FOR THE DETERMINATION OF THE DNA-POLYMERASE SPECIFICITY
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The tridecanucleoside dodecaphosphate d-(T-T-Z-C-G-T-C-A-A-A-A-T-C) incorporating the modified nucleoside 4-amino-1-(2'-deoxy-β-ribofuranosyl)-2(1H)-pyridone (Zd, 1) and the octanucleoside heptaphosphate d(G-A-T-T-T-T-G-A) were synthesized by the solid-phase method using the triester method.The carrier material was a copolymer consisting of styrene with 1 percent divinylbenzene.
- Charczuk, Roland,Tamm, Cristoph
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- Chemical Synthesis of 80-mer Thymidylic Acid
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Chemical synthesis of long-chain oligodeoxyribonucleotides was studied by the method of block condensation in a liquid phase.A method for elongating the chain and the synthesis of oligothymidylic acids up to 80-mer are dscribed.
- Nakahara, Yoshiaki,Ogawa, Tomoya
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p. 2999 - 3004
(2007/10/02)
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- Improved Synthesis of Oligodeoxyribonucleotides by Solid-Phase Phosphotriester Method Utilizing O6--2'-deoxyguanosine Derivatives
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The synthesis of oligodeoxyribonucleotides on a cross-linked polystyrene solid support utilizing stable mono- and dinucleotide phosphotriester building blocks is presented.The use of O6--2'deoxyguanosine derivatives yields cleaner DNA fragments by supressing side reactions.Modifications improving the phosphotriester methodology are presented.The purification methods and analysis of synthetic oligodeoxyribonucleotides are described.
- Chollet, Andre,Ayala, Edgar,Kawashima, Eric H.
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p. 1356 - 1364
(2007/10/02)
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- Simplified Liquid-Phase Preparation of Four Decadeoxyribonucleotides and their Preliminary Spectroscopic Characterization
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Four self-complementary decadeoxyribonucleotides, dApTpApTpApTpApTpApT,dApTpApTpCpGpApTpApT, dApTpApTpGpCpApTpApT and dApApApApApTpTpTpTpT, were chemically synthesized by the phosphotriester procedure.Efforts were concentrated on keeping the procedure as simple as possible, concomitant with obtaining high-purity products at each step of the process.The decamers were elaborated from the 3'-end, starting with a 3'-O-benzoylated monomer according to the scheme: monomer + monomer -> dimer + dimer -> tetramer + dimer -> hexamer + tetramer -> decamer.Putity of intermediates and of the fully blocked decamers were monitored by chromatography and by 300-MHz 1H-NMR. spectroscopy.The deblocked decadeoxyribonucleotides were characterized by their UV., CD., and 1H-NMR. spectra.
- Denny, William A.,Leupin, Werner,Kearns, David R.
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p. 2372 - 2393
(2007/10/02)
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