1372702-84-5

Basic information

• Product Name: β-D-fructofuranosyl-(2→6)-puerarin
• Synonyms: β-D-fructofuranosyl-(2→6)-puerarin
• CAS NO: 1372702-84-5
• Molecular Weight: 578.527
• Mol File: 1372702-84-5.mol
• Article Data: 1

Chemical Properties

• CAS DataBase Reference: β-D-fructofuranosyl-(2→6)-puerarin(CAS DataBase Reference)
• NIST Chemistry Reference: β-D-fructofuranosyl-(2→6)-puerarin(1372702-84-5)
• EPA Substance Registry System: β-D-fructofuranosyl-(2→6)-puerarin(1372702-84-5)

Safety Data

• Hazardous Substances Data: 1372702-84-5(Hazardous Substances Data)

Upstream product

• 3681-99-0 puerarin 
• 57-50-1 Sucrose 

Relevant articles and documents

One-pot bi-enzymatic cascade synthesis of puerarin polyfructosides

Enzymatic glycosylation is an efficient way to increase the water solubility and the bioavailability of flavonoids. Levansucrases from Bacillus subtilis (Bs_SacB), Gluconacetobacter diazotrophicus (Gd_LsdA), Leuconostoc mesenteroides (Lm_LevS) and Zymomonas mobilis (Zm_LevU) were screened for puerarin (daidzein-8-C-glucoside) fructosylation. Gd_LsdA transferred the fructosyl unit of sucrose onto the glucosyl unit of the acceptor forming β-D-fructofuranosyl-(2→6)-puerarin (P1a), while Bs_SacB, Lm_LevS and Zm_LevU synthesized puerarin-4’-O-β-D-fructofuranoside (P1b) and traces of P1a. The Gd_LsdA product P1a was purified and assayed as precursor for the synthesis of puerarin polyfructosides (PPFs). Bs_SacB elongated P1a more competently forming a linear series of water-soluble PPFs reaching at least 21 fructosyl units, as characterized by HPLC-UV-MS, HPSEC and MALDI-TOF-MS. Simultaneous or sequential Gd_LsdA/Bs_SacB reactions yielded PPFs directly from puerarin with the acceptor conversion ranging 82–92 percent. The bi-enzymatic cascade synthesis of PPFs in the same reactor avoided the isolation of the intermediate product P1a and it is appropriate for use at industrial scale.