70393-62-3Relevant articles and documents
Succinct synthesis of saturated hydroxy fatty acids and: In vitro evaluation of all hydroxylauric acids on FFA1, FFA4 and GPR84
Kaspersen, Mads Holmgaard,Jenkins, Laura,Dunlop, Julia,Milligan, Graeme,Ulven, Trond
, p. 1360 - 1365 (2017/07/07)
Saturated hydroxy fatty acids make up a class of underexplored lipids with potentially interesting biological activities. We report a succinct and general synthetic route to saturated hydroxy fatty acids hydroxylated at position 6 or higher, and exemplify this with the synthesis of hydroxylauric acids. All regioisomers of hydroxylauric acids were tested on free fatty acid receptors FFA1, FFA4 and GPR84. The results show that the introduction of a hydroxy group and its position have a high impact on receptor activity.
ALKANE OXIDATION BY MODIFIED HYDROXYLASES
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Paragraph 0342, (2016/02/16)
This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.
A series of hybrid P450 BM3 enzymes with different catalytic activity in the light-initiated hydroxylation of lauric acid
Tran, Ngoc-Han,Huynh, Ngoc,Chavez, Garrett,Nguyen, Angelina,Dwaraknath, Sudharsan,Nguyen, Thien-Anh,Nguyen, Maxine,Cheruzel, Lionel
, p. 50 - 56,7 (2020/07/30)
We have developed a series of hybrid P450 BM3 enzymes to perform the light-activated hydroxylation of lauric acid. These enzymes contain a Ru(II)-diimine photosensitizer covalently attached to single cysteine residues of mutant P450 BM3 heme domains. The library of hybrid enzymes includes four non-native single cysteine mutants (K97C, Q397C, Q109C and L407C). In addition, mutations around the heme active site, F87A and I401P, were inserted in the Q397C mutant. Two heteroleptic Ru(II) complexes, Ru(bpy)2phenA (1) and Ru(phen)2phenA (2) (bpy = bipyridine, phen = 1,10-phenanthroline, and phenA = 5-acetamido-1,10-phenanthroline), are used as photosensitizers. Upon visible light irradiation, the hybrid enzymes display various total turnover numbers in the hydroxylation of lauric acid, up to 140 for the L407C-1 mutant, a 16-fold increase compared to the F87A/Q397C-1 mutant. CO binding studies confirm the ability of the photogenerated Ru(I) compound to reduce the fraction of ferric high spin species present in the mutants upon substrate binding.
Regioselectivity and Activity of Cytochrome P450 BM-3 and Mutant F87A in Reactions Driven by Hydrogen Peroxide
Cirino, Patrick C.,Arnold, Frances H.
, p. 932 - 937 (2007/10/03)
Cytochrome P450 BM-3 (EC 1.14.14.1) is a monooxygenase that utilizes NADPH and dioxygen to hydroxylate fatty acids at subterminal positions. The enzyme is also capable of functioning as a peroxygenase in the same reaction, by utilizing hydrogen peroxide i
Asymmetric Synthesis of Hydroxy Carboxylic Acids
Midland, M. Mark,Lee, Penny E.
, p. 3933 - 3934 (2007/10/02)
Optically-active hydroxy carboxylic acids of the general formula RCHOH(CH2)nCO2H are prepared from optically-active propargyl alcohols.
Occurence of 7-Hydroxyalkanoic Acids in Mucor Species
Tahara, Satoshi,Hosokawa, Keizo,Mizutani, Junya
, p. 193 - 198 (2007/10/02)
Three 7-hydroxyalkanoic acids not previously reported in microorganisms were found in extracts from saponified whole cells of Mucor spp. grown under oxygen limiting conditions.On the basis of evidence from mass spectrometry, infrared spectroscopy, gas chromatographic procedures and chemical conversions, they were confirmed to be 7-hydroxydecanoic, 7-hydroxydodecanoic and 7-hydroxytetradecanoic acids.The racemates of the former two acids were synthesized and compared with the isolates.These acids were found in the hydrolyzates of extractable and residual parts of lyophilized cells with a chloroform-methanol (2:1) mixture.The hydroxy acid contents of eight species of the genus Mucor grown in stationary liquid medium were also determined.
