The Cholesterol oxidase, with the CAS registry number 9028-76-6, is also known as 3beta-hydroxy steroid oxidoreductase. It belongs to the product category of Enzymes. Its EINECS number is 232-842-1. Its EC number is 1.1.3.6. What's more, its systematic name is cholesterol:oxygen oxidoreductase. Its classification code is TSCA Flag XU [Exempt from reporting under the Inventory Update Rule]. It should be sealed and stored in a cool and dry place with the storage temperature is 2-8°C. In enzymology, it is an enzyme that catalyzes the chemical reaction: cholesterol + O₂ cholest-4-en-3-one + H₂O₂. Thus, the two substrates of this enzyme are cholesterol and O₂, whereas its two products are cholest-4-en-3-one and H₂O₂. This enzyme belongs to the family of oxidoreductases. This enzyme participates in bile acid biosynthesis. The substrate-binding domain found in some bacterial Cholesterol oxidases is composed of an eight-stranded mixed beta-pleated sheet and six alpha-helices. This domain is positioned over the isoalloxazine ring system of the FAD cofactor bound by the FAD-binding domain and forms the roof of the active site cavity, allowing for catalysis of oxidation and isomerisation of cholesterol to cholest-4-en-3-one.