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Name	Direct Blue 53	EINECS	206-242-5
CAS No.	314-13-6	Density	N/A
PSA	404.26000	LogP	10.78520
Solubility	280g/L (20 °C) in water	Melting Point	N/A
Formula	C₃₄H₂₄N₆Na₄O₁₄S₄	Boiling Point	N/A
Molecular Weight	960.84	Flash Point	N/A
Transport Information	N/A	Appearance	brownish crystalline powder
Safety	53-45-36/37-16-36/37/39	Risk Codes	45-63-11-61-20/21/22
Molecular Structure		Hazard Symbols	T, F
Synonyms	1,3-Naphthalenedisulfonicacid,6,6'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4-amino-5-hydroxy-,tetrasodium salt (9CI);Azovan Blue (6CI);C.I. Direct Blue 53, tetrasodiumsalt (8CI);4,4'-Bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3'-bitolyltetrasodium salt;C.I. 23860;C.I. Direct Blue 53;Diazol Pure Blue BF;Evans Blue;Evan's Blue;T 1824;

Direct Blue 53 Chemical Properties

IUPAC Name: Tetrasodium(6E)-4-amino-6-[[4-[4-[(2E)-2-(8-amino-1-oxo-5,7-disulfonatonaphthalen-2-ylidene)hydrazinyl]-3-methylphenyl]-2-methylphenyl]hydrazinylidene]-5-oxonaphthalene-1,3-disulfonate
Molecular Formula: C₃₄H₂₄N₆Na₄O₁₄S₄
Molecular Weight: 960.80 g/mol
EINECS: 206-242-5
Classification Code: Coloring Agents; Drug / Therapeutic Agent; Mutation data; Reproductive Effect; Tumor data
Product Categories: Organics; Glutamate receptor
Stability: Stable. Incompatible with strong reducing agents, strong oxidizing agents.
storage temperature: Store at RT.
Water Solubility of Evans blue (CAS NO.314-13-6): 280 g/L (20 °C)

Direct Blue 53 Uses

Evans blue (CAS NO.314-13-6) is an azo dye used in blood volume and cardiac output measurement by the dye dilution method. It is very soluble, strongly bound to plasma albumin, and disappears very slowly.

Direct Blue 53 Toxicity Data With Reference

Organism	Test Type	Route	Reported Dose (Normalized Dose)	Effect	Source
cat	LDLo	intravenous	1mL/kg (1mL/kg)	GASTROINTESTINAL: "HYPERMOTILITY, DIARRHEA" LIVER: OTHER CHANGES KIDNEY, URETER, AND BLADDER: "CHANGES IN TUBULES (INCLUDING ACUTE RENAL FAILURE, ACUTE TUBULAR NECROSIS)"	Archives of Surgery Vol. 48, Pg. 17, 1944.
dog	LDLo	intravenous	3mL/kg (3mL/kg)	GASTROINTESTINAL: "HYPERMOTILITY, DIARRHEA" GASTROINTESTINAL: NAUSEA OR VOMITING LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA	Archives of Surgery Vol. 48, Pg. 17, 1944.
mouse	LD50	intraperitoneal	340mg/kg (340mg/kg)		Biochemical and Biophysical Research Communications. Vol. 136, Pg. 64, 1986.
rabbit	LDLo	intravenous	1mL/kg (1mL/kg)	CARDIAC: OTHER CHANGES LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA	Archives of Surgery Vol. 48, Pg. 17, 1944.
rat	LDLo	intravenous	5mL/kg (5mL/kg)	CARDIAC: OTHER CHANGES LUNGS, THORAX, OR RESPIRATION: ACUTE PULMONARY EDEMA	Archives of Surgery Vol. 48, Pg. 17, 1944.

Direct Blue 53 Consensus Reports

IARC Cancer Review: Group 3 IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man 7 (1987),p. 56.(World Health Organization, Internation Agency for Research on Cancer,Lyon. France.: ) (single copies can be ordered from WHO Publications centre U.S.A., 49 Sheridan Avenue, Albany, NY 12210) ; Animal Limited Evidence IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man 8 (1975),p. 151.(World Health Organization, Internation Agency for Research on Cancer,Lyon. France.: ) (single copies can be ordered from WHO Publications centre U.S.A., 49 Sheridan Avenue, Albany, NY 12210) . Reported in EPA TSCA Inventory. EPA Genetic Toxicology Program.

Direct Blue 53 Safety Profile

Poison by intraperitoneal route. Moderately toxic by intravenous route. An experimental teratogen. Other experimental reproductive effects. Questionable carcinogen with experimental tumorigenic data. Mutation data reported. When heated to decomposition it emits very toxic fumes of SO_x, Na₂O, and NO_x.
Hazard Codes: Toxic T,F
Risk Statements: 45-63-11-61-20/21/22
R45:May cause cancer.
R63:Possible risk of harm to the unborn child.
R11:Highly flammable.
R61:May cause harm to the unborn child.
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed.
Safety Statements: 53-45-36/37-16-36/37/39
S53:Avoid exposure - obtain special instructions before use.
S45:In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
S36/37:Wear suitable protective clothing and gloves.
S16:Keep away from sources of ignition.
S36/37/39:Wear suitable protective clothing, gloves and eye/face protection.
WGK Germany: 3
RTECS of Evans blue (CAS NO.314-13-6): QJ6440000

Direct Blue 53 Specification

Evans blue (CAS NO.314-13-6), its Synonyms are 1,3-Naphthalenedisulfonic acid, 6,6'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(2,1-diazenediyl))bis(4-amino-5-hydroxy-, sodium salt (1:4) ; 1,3-Naphthalenedisulfonic acid, 6,6'-((3,3'-dimethyl(1,1'-biphenyl)-4,4'-diyl)bis(azo))bis(4-amino-5-hydroxy-, tetrasodium salt ; 1,3-Naphthalenedisulfonic acid, 6,6'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo))bis(4-amino-5-hydroxy-, tetrasodium salt ; C.I. Direct Blue 53 ; C.I. Direct Blue 53, tetrasodium salt (8CI) ; Tetrasodium 6,6'-(3,3'-dimethyl-(1,1'-biphenyl)-4,4'diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate) . It is Blue crystals with a greenish-bronze luster or a black powder.

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