Chemical constituents of the bark of Machilus wangchiana and their biological activities

Article abstract of DOI:10.1021/np900504a

Eleven new metabolites, butanolides 1-6, lignan derivatives 7-9, sesquiterpene 10, and 3′,4′-seco-flavane derivative 11, have been isolated from an ethanol extract of Machilus wangchiana. Twenty known compounds, including ginkgolides A and B (16 and 17),

Full text of DOI:10.1021/np900504a

J. Nat. Prod. 2009, 72, 2145–2152
2145
Chemical Constituents of the Bark of Machilus wangchiana and Their Biological Activities
Wei Cheng, Chenggen Zhu, Wendong Xu, Xiaona Fan, Yongchun Yang, Yan Li, Xiaoguang Chen, Wenjie Wang, and
Jiangong Shi*
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College (Key Laboratory of BioactiVe
Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education), Beijing 100050, People’s Republic of China
ReceiVed August 16, 2009
Eleven new metabolites, butanolides 1-6, lignan derivatives 7-9, sesquiterpene 10, and 3′,4′-seco-flavane derivative
11, have been isolated from an ethanol extract of Machilus wangchiana. Twenty known compounds, including ginkgolides
A and B (16 and 17), were also isolated. Their structures and absolute configurations were determined by spectroscopic
and chemical methods. Compounds 7, 8a, 8b, 9, 11, (+)-guaiacin (12), meso-dihydroguaiaretic acid (13), and
hamabiwalactone A (15) showed potent in vitro activities against the release of ꢀ-glucuronidase in rat polymorphonuclear
leukocytes (PMNs) induced by platelet-activating factor (PAF), with 42.5-75.6% inhibition at 10-5 M. Compounds 8,
8a, 8b, 9, and 11 reduced DL-galactosamine (GalN)-induced hepatocyte (WB-F344 cells) damage with 39.4 ( 6.3% to
53.6 ( 3.5% inhibition at 10-4 M. Isomahubannolide-23 (14) was cytotoxic against human stomach cancer (BGC-823)
and ovarian cancer (A2780) cell lines, with IC50 values of 0.13 and 2.66 µM, respectively.
Species of the genus Machilus are widely distributed in
southeastern Asia, especially in southern China.1 Lignans,2 bu-
tanolides,3 sesquiterpenes,3 alkaloids,4 and flavonoids5 have been
reported from several plants of this genus. As part of a program to
study traditional Chinese medicines,6 an ethanolic extract of the
bark of Machilus wangchiana Chun. (Lauraceae) was investigated.
In this paper, we describe the isolation, structural elucidation, and
some in vitro bioassays of 11 new compounds, butanolides (1-6),
lignan derivatives (7-9), a sesquiterpene (10), and a 3′,4′-seco-
flavan derivative (11), and 20 known compounds from this material.
It is of interest to note that Ginkgo biloba (maidenhair) metabolites,
ginkgolides A (16) and B (17),7 were present in this material.
Results and Discussion
Compound 1, a colorless oil, [R]20D +26 (c 0.11, CHCl3), showed
the presence of hydroxy (3341 cm-1), alkyne (2117 cm-1), and
lactone (1738 and 1678 cm-1) functional groups in its IR spectrum.
The molecular formula (C17H26O3) was determined by HRESIMS.
The 1H NMR spectrum showed signals similar to those of litsenolide
8
B2 and lincomolide B9 at δ 6.98 (t, J ) 7.5 Hz, H-6), 4.53 (1H,
brs, H-3), 4.50 (1H, q, J ) 6.5 Hz, H-4), 2.18 (dt, J ) 2.0, 7.0 Hz,
H-15), 1.94 (d, J ) 2.0 Hz, H-17), and 1.34 (d, J ) 6.5 Hz, H-5)
(Table 1). The chemical shifts and coupling patterns of H-3 and
H-4 suggested that the relative configuration of 1 was identical to
that of litsenolide B2. This conclusion was supported by comparison
of the 13C NMR data of 1 (Table 2) with those of reported
compounds having a trans-relationship between H-3 and H-4.8 The
chemical shift for C-5 of 1 (δ 19.7) and C-5 of the cis-form occurs
tridecanol]-furan in the literature.10c However, the hydrogenated
product (1a) was esterified successively by R-(-)- and S-(+)-
MTPACl to give the corresponding S-MTPA and R-MTPA deriva-
tives. The 1H NMR data of the diastereomers (Experimental Section)
near δ 14.0.8b,9,10 The positive [R]20D of 1 suggested that its absolute
configuration was opposite that of litsenolide B2.8a,11 This was
verified by chemical transformation12 and by the modified Mosher
method13 (Supporting Information, Scheme S1). Hydrogenation of
1 (Pd/C) yielded 1a. Acetylation of 1a followed by elimination of
the acetoxy group yielded 1c {[R]20D +27 (c 0.12, CHCl3)}, having
spectroscopic data consistent with those of (+)-(5S)-3-dodecyl-5-
1
were assigned on the basis of their H-1H COSY experiments.
From the MTPA determination rule,13,16 it was determined that 1a
has a 3R configuration. Thus, 1 was determined to be (+)-
(2E,3R,4S)-2-(dodec-11-ynylidene)-3-hydroxy-4-methylbutano-
lide.
Compound 2 had the molecular formula C17H28O3, two hydrogen
atoms more than that of 1 (HRESIMS). The IR spectrum of 2
resembled that of 1; however, the absence of the alkyne absorption
suggested that 2 was a 16,17-dihydrogenated analogue of 1. This
was supported by a replacement of the NMR resonances for the
ethynyl unit of 1 by those for a terminal vinyl group of 2 [δH 5.80
(ddt, J ) 17.0, 10.0, and 7.0 Hz, H-16), 4.98 (d, J ) 17.0 Hz,
methylfuran-2(5H)-one {[R]25 +24.0 (c 0.2, dioxane)}.14 Esteri-
D
fication of 1 with R-(-)- and S-(+)-R-methoxy-R-(trifurometh-
yl)phenylacetyl chloride (MTPACl) under normal conditions15
failed to give the MTPA esters of 1, but instead gave the
decarboxylation product 1d, (E)-hexadeca-3-ene-15-ynyl-2,5-dione,
which was identified incorrectly as 2-hydroxy-5-methyl-3-[1-
* To whom correspondence should be addressed. Tel: 86-10-83154789.
Fax: 86-10-63017757. E-mail: shijg@imm.ac.cn.
10.1021/np900504a  2009 American Chemical Society and American Society of Pharmacognosy
Published on Web 11/16/2009

2146 Journal of Natural Products, 2009, Vol. 72, No. 12
Cheng et al.
Table 1. 1H NMR Spectroscopic Data (δ) of Compounds 1-6 (CDCl3, 500 MHz)a
no.
1
2
3
4
5
6
3
4
5
6
4.53 brs
4.52 brs
4.35 brs
4.33 brs
4.65 d (4.5)
4.55 dq (4.5, 6.5)
1.40 d (6.5)
6.56 t (7.5)
4.56 d (1.5)
4.50 q (6.5)
1.34 d (6.5)
6.98 t (7.5)
2.40 m
4.50 q (6.5)
1.33 d (6.5)
6.95 t (8.0)
2.39 m
4.38 q (6.0)
1.38 d (6.0)
6.53 t (7.5)
2.75 m
4.32 q (6.0)
1.38 d (6.0)
6.53 t (7.5)
2.74 m
4.50 dq (1.5, 6.5)
1.35 d (6.0)
7.01 t (7.5)
2.40 m
7
2.75 m
15
16
17
2.18 dt (2.0, 7.0)
2.02 q (7.0)
5.80 ddt (17.0, 10.0, 7.0)
4.98 d (17.0);
2.02 q (7.0)
5.81 ddt (17.5, 10.0, 7.0)
4.99 d (17.5);
2.17 dt (2.5, 7.0)
2.18 dt (2.0, 7.0)
1.94 t (2.0)
1.93 t (2.5)
1.94 t (2.0)
4.92 d (10.0)
4.92, d (10.0)
19
0.88 t (7.0)
a Proton coupling constants (J) in Hz are given in parentheses. The methylene proton resonances at positions 8-14 were overlapped between δ 1.25
and 1.55 for 1, 2, and 4, between δ 1.25 and 1.50 for 3, and between δ 1.25 and 1.65 for 5, while the methylene protons at positions 8-18 of 6 were
overlapped between δ 1.25 and 1.60.
Table 2. 13C NMR Spectroscopic Data (δ) of Compounds 1-6
demonstrated a 4S configuration for 5. The negative specific
(CDCl3, 125 MHz)a
rotation of 5 {[R]20 -26 (c 0.10, CHCl3)} was consistent with
D
thoseof(3S,4S)-2-alkylidene-3-hydroxy-4-methylbutanolides.9,10d,11,18
Thus, the structure of 5 was assigned as (-)-(2Z,3S,4S)-2-(dodec-
11-ynylidene)-3-hydroxy-4-methylbutanolide.
no.
1
2
3
4
5
6
1
2
3
4
5
6
7
15
16
17
18
19
169.4
129.3
82.5
72.2
19.7
148.6
29.7
18.4
84.7
68.1
169.7
129.3
82.6
72.2
19.7
147.7
29.7
33.8
139.2
114.1
168.0
128.8
81.2
75.6
19.1
149.3
27.8
33.8
139.2
114.1
168.1
128.8
81.3
75.6
19.1
149.2
27.7
18.4
84.8
68.1
168.5
129.3
77.7
71.4
14.1
149.6
27.9
18.4
84.8
68.0
169.8
129.3
82.4
72.2
19.7
Compound 6, a colorless oil, displayed IR, MS, and NMR data
almost identical to those of the natural product litsenolide C18a and
the synthetic ent-litsenolide C1.19 However, the specific rotation
of 6 {[R]20D +30} was in good agreement with that of ent-litsenolide
C1. Therefore, 6 was identified as ent-litsenolide C1, a new natural
product.
148.7
31.9
The IR spectrum of compound 7 indicated the presence of
hydroxy (3366 cm-1) and aromatic ring (1611, 1502, 1460 cm-1
)
22.7
14.1
groups. (+)-ESIMS of 7 gave a quasimolecular ion peak at m/z
397 [M + K]+, and HRESIMS indicated a molecular formula of
C21H26O5. The 1H NMR spectrum exhibited signals attributable to
a trisubstituted aromatic ring [δ 6.61 (d, J ) 1.5 Hz, H-2′), 6.73
(d, J ) 8.0 Hz, H-5′), and 6.37 (dd, J ) 8.0, 1.5 Hz, H-6′)], a
pentasubstituted aromatic ring [δ 6.44 (1H, s, H-6)], and three
methoxy groups. In addition, it displayed two methyl doublets [δ
0.88 (d, J ) 7.0 Hz, H3-9) and 0.91 (d, J ) 7.5 Hz, H3-9′)], a
methine doublet [δ 4.05 (d, J ) 2.0 Hz, H-7′)], and two methine
mutiplets [δ 2.02 (H-8) and 1.85 (H-8′)], as well as signals
attributable to germinal protons of a methylene attached to a methine
[δ 2.70 (dd, J ) 16.5, 6.0 Hz, H-7a) and 2.39 (dd, J ) 16.5, 11.5
Hz, H-7b)]. These data suggested that 7 was a 2,7′-cyclolignan
derivative with three methoxy and two hydroxy groups substituted
at the aromatic rings, which was confirmed by the 13C NMR
(Experimental Section) and HMBC (Supporting Information, Figure
S1) data of 7. In particular, HMBC correlations from both MeO-3
and H-7′ to C-3, from MeO-5 to C-5, from H-6 to C-1, C-2, C-4,
C-5, and C-7, from both MeO-3′ and H-5′ to C-3′, and from H-2′
to C-1′, C-3′, C-4′, and C-6′, together with the chemical shifts of
these protons and carbons, indicated that the three methoxy and
two hydroxy groups were located at C-3, C-5, and C-3′, and C-4
and C-4′, respectively. In the NOESY spectrum of 7, cross-peaks
between MeO-3 and H-7′, between MeO-5 and H-6, and between
MeO-3′ and H-2′ (Supporting Information, Figure S2) confirmed
the locations of the three methoxy groups. In addition, NOESY
cross-peaks of both H-8 and H-8′ with H-2′ and H-6′ indicated
that these protons were oriented on the same side of the tetralin
ring, while the trans-relationship between H-7′ and H-8′ was
supported by the coupling constant J7,8 (2.0 Hz).20 On the basis of
the CD exciton chirality rule, in the CD spectrum of 7, a negative
Cotton effect at 274.5 nm (∆ε -1.87) and a positive Cotton effect
at 289 nm (∆ε +0.16), opposite those of the co-occurring (+)-
guaiacin (12)21 (Supporting Information, Figure S3), indicated that
7 had a 7′R configuration. Therefore, 7 was determined to be
(-)-(7′R,8R,8′R)-4,4′-dihydroxy-3,3′,5-trimethoxy-2,7′-cyclolign-
ane.22b
a The methylene carbon resonances at positions 8-14 were
overlapped between δ 18.4 and 29.3 for 1, 4, and 5, between δ 28.4 and
29.4 for 3, and between δ 28.8 and 29.4 for 5. The methylene carbon
resonances at positions 8-17 of 6 were overlapped between δ 28.4 and
29.7.
H-17a), and 4.92 (d, J ) 10.0 Hz, H-17b), and δC 139.2 (C-16)
and 114.1 (C-17)]. In addition, the NMR data for H-3 and H-4 and
C-5 of 2 (Tables 1 and 2), together with the positive [R]20D, indicated
3R,4S configuration for 2. The configuration was confirmed by
chemical transformation from 2 to 1c using the same procedure as
described above. Therefore, 2 was determined to be (+)-(2E,3R,4S)-
2-(dodec-11-enylidene)-3-hydroxy-4-methylbutanolide, an enanti-
8a
omer of litsenolide A2 or the 3-epimer of lincomolide C.10d
Compound 3 displayed spectroscopic data similar to those of 2
(Tables 1 and 2, and Experimental Section). However, H-3, H-4,
and H-6 of 3 were shielded by ∆δH -0.17, -0.12, and -0.42 ppm,
respectively, as compared with those of 2, whereas H-5 and H2-7
of 3 were deshielded by ∆δH +0.05 and +0.36 ppm, respectively.
In addition, C-1, C-3, and C-7 of 3 were shielded by ∆δC -1.7,
-1.4, and -1.9 ppm, respectively; in turn C-4 and C-6 were
deshielded by ∆δC +3.4 and +1.6 ppm, respectively. These
differences indicated that 3 was a 2Z-isomer of 2,8a,11,17 which was
confirmed also by chemical transformation from 3 to 1c. Accord-
ingly, 3 was determined to be (+)-(2Z,3R,4S)-2-(dodec-11-enylidene)-
3-hydroxy-4-methylbutanolide. It is an enantiomer of litsenolide
A1.8a
Compound 4 showed IR and ESIMS data (Experimental Section)
almost identical to those of 1. The differences in the NMR data
between 4 and 1 (Tables 1 and 2) were similar to those between 3
and 2. This suggested that 4 was a 2Z-isomer of 1, which was also
confirmed by chemical transformation of 4 to 1c. Therefore, the
structure of 4 was assigned as (+)-(2Z,3R,4S)-2-(dodec-11-ynylidene)-
3-hydroxy-4-methylbutanolide.
The spectroscopic data of 5 revealed that it was an isomer of
4. The chemical shifts and coupling patterns for H-3 [δ 4.65 (d,
J ) 4.5 Hz)] and H-4 [δ 4.55 (dd, J ) 4.5, 6.5 Hz)] of 5 and
the shift for C-5 (δ 14.1) indicated that H-3 and H-4 are cis-
oriented in 5.8b,9,10c-e Chemical transformation from 5 to 1c
For compound 8, [R]20 +15 (c 0.05, CHCl3), IR absorptions
D
for OH (3291 cm-1), carbonyl (1737 cm-1), and aromatic ring

Chemical Constituents of Machilus wangchiana
Journal of Natural Products, 2009, Vol. 72, No. 12 2147
(1462, 1377 cm-1) groups, and (+)-HRESIMS indicated the
molecular formula C12H16O4. The 1H NMR spectrum showed
resonances assignable to a 4-hydroxy-3-methoxyphenyl unit at δ
6.88 (d, J ) 1.5 Hz, H-2), 6.88 (d, J ) 8.0 Hz, H-5), 6.80 (dd, J
) 8.0, 1.5 Hz, H-6), and 3.91 (s, MeO-3), an O-CH-CH-CH3 unit
at δ 4.68 (d, J ) 9.0 Hz, H-7), 2.89 (dq, J ) 9.0, 7.5 Hz, H-8),
and 0.93 (d, J ) 7.5 Hz, H3-9), and an acetyl unit at δ 2.24 (s,
H3-9′). These data suggested that 8 possessed a planar structure of
4-hydroxy-3-methoxy-1′,2′,3′,4′,5′,6′,7′-heptanorlign-8′-one.22,23 The
13C NMR data of 8 (Experimental Section) were consistent with
the structural assignment. The coupling constant between H-7 and
H-8 (J7,8 ) 9.0 Hz) and the resonances for C-7 (δ 76.5), C-8 (δ
53.9), and C-9 (δ 14.2) indicated a threo-relationship between the
OH group at C-7 and the acetyl unit at C-8 in 8.24 The positive
their corresponding protons were assigned by the HSQC experiment.
1H-1H COSY cross-peaks between H-4 and both H2-3 and H3-14
and HMBC correlations from H2-3 to C-1, C-2, C-4, and C-5 and
from H3-14 to C-3, C-4, and C-5, in combination with the coupling
patterns and chemical shifts of these protons and carbons, revealed
the presence of a 1,5-disubstituted 4-methylcyclopent-5(1)-en-2-
one moiety in 10. A series of 1H-1H COSY cross-peaks from H2-6
through H-7 to H2-8 and then to H2-9, H-10, and H3-15, and HMBC
correlations from H2-6 to C-1, C-5, C-7, and C-8 and from H3-15
to C-1, C-9, and C-10, together with chemical shifts of these protons
and carbons, demonstrated the presence of a seven-membered ring
in 10. HMBC correlations from H2-13 to C-7, C-11, and C-12, along
with their shifts and the molecular composition, located an acrylic
acid unit at C-7 in 10. Therefore, 10 was a 2-oxo-guaia-1(5),11(13)-
dien-12-oic acid.
[R]20 indicated that the absolute configuration of 8 is 7S,8R.25
D
Therefore, the structure of 8 was elucidated as (+)-(7S,8R)-4-
hydroxy-3-methoxy-1′,2′,3′,4′,5′,6′,7′-heptanorlign-8′-one. The meth-
yl and ethyl ethers of 8 (8a and 8b) were also obtained in the
isolation procedure; however, they were considered artifacts since
storage of 8 in methanol or ethanol at 40 °C for 24 h also yielded
8a or 8b.
The absolute configuration of 10 was determined by analysis of
NOE difference combined with the modified Mosher method.
Irradiation of H3-14 gave enhancements of H-3b, H-6b, and H-7,
while irradiation of H3-15 enhanced H-7, H-8a, and H-9a. These
data indicated that the methyl groups at C-4 and C-10 were on the
same side of the ring system and that the acrylic acid unit at C-7
was on the other side. Methylation of 10 followed by successive
hydrogenation (Pd-C/H2), reduction (NaBH4), and esterification
(R- or S-MTPACl) yielded two pairs of diastereomers, 10bR and
10bS and 10cR and10cS (Supporting Information, Scheme S3).
After a careful assignment of the 1H NMR data of the two pairs of
diastereomers by 1H-1H COSY and NOE data analysis, chemical
shift differences ∆δSR (δS-MTPAester - δR-MTPAester) of the two pairs
of diastereomers were obtained (Supporting Information, Scheme
S3). On the basis of the MTPA determination rule,13,16 the ∆δSR
values indicated a 2R configuration for both 10b and 10c. In the
NOE difference experiments of 10bR, irradiation of H-2 enhanced
H-1, H-4, and H-3a, indicating that these protons were on the same
side of the ring system. This suggested that the absolute configu-
ration at C-4 of 10b, 10c, and 10 is S. Therefore, 10 was determined
to be (-)-(4S,7S,10S)-2-oxo-guaia-1(5),11(13)-dien-12-oic acid.
Compound 11 (C15H14O8) showed IR absorptions for OH (3359
cm-1), carbonyl (1786 and 1728 cm-1), and aromatic ring (1627,
1522, and 1468 cm-1) groups. 1H NMR resonances at δ 6.05 (d, J
) 2.0 Hz, H-6) and 5.91 (d, J ) 2.0 Hz, H-8) indicated a
tetrasubstituted aromatic ring with two meta-coupling protons. An
ABX system at δ 4.78 (d, J ) 6.8 Hz, H-6′), 3.19 (dd, J ) 18.4,
6.8 Hz, H-5′a), and 2.50 (d, J ) 18.4 Hz, H-5′b) indicated the
presence of an O-CHCH2 unit. In addition to a singlet assignable
to an isolated methylene unit at δ 3.20 (s, H2-2′), resonances due
to a vicinal coupling system at δ 4.45 (d, J ) 1.6 Hz, H-2), 4.52
(dt, J ) 4.8, 1.6 Hz, H-3), 2.91 (dd, J ) 18.0, 1.6 Hz, H-4a), and
2.76 (dd, J ) 18.0, 4.8 Hz, H-4b) suggested the presence of a
CH(O)CH(O)CH2 unit. These units were confirmed by the 1H-1H
COSY data of 11. The 13C NMR and DEPT spectra of 11 also
showed resonances of two carbonyl groups and an oxygen-bearing
quaternary carbon. The protonated carbon resonances were assigned
from the HMQC experiment. In the HMBC spectrum of 11, two-
and three-bond correlations from both H-6 and H-8 to C-4a and
from H2-4 to C-2, C-3, C-4a, C-5, and C-8a indicated a connection
between C-4a and C-4. Correlations from H-2 to C-1′ and C-6′
and from H-2′ to C-2, C-1′, and C-3′ indicated connection of C-1′
to both C-2 and C-2′ and C-2′ to C-3′. Correlations from H2-5′ to
C-1′, C-4′, and C-6′ revealed that C-6′ connected to both C-1′ and
C-5′. In addition, a correlation from H-6′ to C-3 and the shifts of
H-6′ and C-3 suggested an oxygen-bridged linkage between C-3
and C-6′. Methylation of 11 with CH3I gave a trimethyl product,
11a. Comparison of the NMR data between 11a and 11 (Experi-
mental Section) indicated that H-6 and H-8 and C-4a, C-5, and
C-7 of 11a were deshielded by ∆δH +0.10 and +0.15 and ∆δC
+6.9, +2.4, and +3.0 ppm, respectively, whereas C-6, C-8, and
C-3′ were shielded by ∆δC -2.3, -3.2, and -1.2 ppm, respectively.
HRESIMS indicated compound 9 had the molecular formula
1
C13H16O5. The H NMR data of 9 were similar to those of 8.
However, resonances for H-7 and H3-9 of 9 were deshielded by
∆δH +0.39 and +0.15, respectively, as compared with those of
8, whereas those for H-8 and H3-9′ of 9 were significantly
shielded by ∆δH -0.85 and -0.74, respectively. Comparison
of the 13C NMR spectra between 8 and 9 indicated that the ketone
carbonyl (δC 213.4, C-8′) of 8 was replaced by an ester carbonyl
in 9 (δC 177.5, C-7′). This was supported by a diagnostic
absorption due to a γ-lactone carbonyl (1768 cm-1) in the IR
spectrum of 9. In addition, the 13C NMR spectrum of 9 showed
an additional resonance attributable to an oxygen-bearing
quaternary carbon (δC 74.4, C-8′). These data suggested that 9
was a 4,8′-dihydroxy-3-methxy-1′,2′,3′,4′,5′,6′-hexanorligna-7′,7-
lactone,22,26 which was supported by 2D NMR experiments. In
the HMBC spectrum of 9, long-range correlations (Supporting
Information, Figure S1) from H3-9 to C-7, C-8, and C-8′ and
from H3-9′ to C-7′, C-8, and C-8′, in combination with chemical
shifts of these protons and carbons, indicated the presence of
the lactone moiety. HMBC correlations from H-7 to C-1, C-2,
C-6, C-8, and C-9 confirmed the connection between the phenyl
unit and the lactone moiety, while correlations from both H-5
and MeO to C-3 proved the methoxy to be on the phenyl moiety.
In the NOE difference spectrum of 9, H3-9 was enhanced by
irradiation of H-7, while H3-9′ was enhanced by irradiation of
H-8. These enhancements indicated a trans-orientation of H-8
with H-7 and HO-8′ on the lactone moiety of 9. The absolute
configuration of 9 was determined by conversion of 9 to 8.
Reduction of 9 with LiAlH4 followed by oxidation of the product
with NaIO4 yielded 8 (Supporting Information, Scheme S2). The
spectroscopic data and specific rotation of semisynthetic 8 were
identical to those of 8. Thus, 9 was determined to be (+)-
(7S,8R,8′R)-4,8′-dihydroxy-3-methoxy-1′,2′,3′,4′,5′,6′-hexanor-
ligna-7′,7-lactone.22
Compound 10 had the molecular formula C15H20O3 (HRESIMS).
The 1H NMR spectrum of 10 showed two broad singlets attributable
to an olefinic methylene at δ 6.38 (s, H-13a) and 5.76 (s, H-13b),
two methyl doublets at δ 1.11 (d, J ) 7.5 Hz, H3-14) and 1.03 (d,
J ) 7.0 Hz, H3-15), and partially overlapped multiplets due to
aliphatic methylenes and/or methines between δ 1.50 and 3.10. The
13C NMR spectrum of 10 displayed 15 carbon resonances consisting
of two methyls, five methylenes (one sp2), three methines, and five
quaternary carbons (a ketone, a carboxyl, and three olefinic). These
data indicated that 10 was a bicyclic sesquiterpene with functional
groups of a ketone, a carboxyl, a disubstituted terminal double bond,
and a tetrasubstituted double bond. The protonated carbons and

2148 Journal of Natural Products, 2009, Vol. 72, No. 12
Cheng et al.
cuToFCS JMS-T100CS spectrometer. EIMS and HREIMS data were
measured with a Micromass Autospec-Ultima ETOF spectrometer.
Column chromatography (CC) was performed using silica gel (200-300
mesh, Qingdao Marine Chemical Inc. Qingdao, People’s Republic of
China) and Pharmadex LH-20 (Amersham Biosciences, Inc., Shanghai,
People’s Republic of China). HPLC separation was performed on an
instrument consisting of a Waters 600 controller, a Waters 600 pump,
and a Waters 2487 dual λ absorbance detector, using a Prevail (250 ×
10 mm i.d.) column packed with C18 (5 µm). Preparative TLC separation
was performed with high-performance silica gel preparative TLC plates
(HSGF254, glass precoated, Yantai Jiangyou Silica Gel Development
Co., Ltd., Yantai, People’s Republic of China). TLC was carried out
with glass precoated silica gel GF254 plates. Spots were visualized under
UV light or by spraying with 7% H2SO4 in 95% EtOH followed by
heating. Unless otherwise noted, all chemicals were obtained from
commercially available sources and were used without further purification.
Plant Material. The bark of M. wangchiana was collected at Dayao
Moutain, Guangxi Province, China, in August 2006. The plant was
identified by Mr. Guang-Ri Long (Guangxi Forest Administration,
Guangxi 545005, China). A voucher specimen (no. 06009) was
deposited at the Herbarium of the Department of Medicinal Plants,
Institute of Materia Medica.
This suggested the presence of free phenolic OH groups at C-5
and C-7 and a free carboxylic OH group at C-3′ in 11. The HMBC
spectrum of 11a showed correlations from proton resonances of
the three methoxy groups to C-5, C-7, and C-3′, respectively. The
chemical shifts of C-2, C-8a, C-1′, and C-4′, together with the
molecular formula of 11, indicated that a lactone and an oxygen-
bridged linkage were among these carbons. In the IR spectrum of
11, the characteristic absorption for a γ-lactone ring at 1786 cm-1
demonstrated that the lactone must be formed between C-4′ and
C-1′ and the oxygen-bridged linkage between C-2 and C-8a in 11.
Thus, 11 was deduced to be an unusual 3′,4′-seco-flavane derivative
as shown. The configuration of 11 was proposed from the NMR
1
data and a biogenetic hypothesis. In the H NMR spectra of 11
and 11a, the respective coupling constants of 1.6 and 2.4 Hz
between H-2 and H-3 indicated a cis-orientation for the two protons
in the two compounds. In the NOE difference experiment of 11a,
irradiation of H-6′ gave an enhancement of H-2′; however, no
enhancement of H-2′ and/or H-6′ was observed by irradiation of
H-2 or H-3. This suggested that H-2′ and H-6′, opposite H-2 and
H-3, were on the same side of the ring. Although three closely
related analogues, viniferones A-C, and a plausible biogenesis of
viniferone A (C-3 epimer of viniferone B) from (+)-catechin,
mediated by catechin quinone and followed by a sequential
oxidative cleavage, hydration, and lactonization pathway or by a
sequential hydration, oxidative cleavage, and lactonization pathway,
were reported,27 we hypothesize that 11 and viniferones A and C
may be biosynthesized from an enzyme-catalyzed oxidative cleav-
age between C-3′ and C-4′ of the co-occurring (-)-epicatechin
followed by a sequential or simultaneous cycloaddition procedure
(Supporting Information, Scheme S4). Therefore, the structure of
11 was proposed as (+)-(2S,3R,1′S,6′R)-5,7-dihydroxy-3,6′-epoxy-
1′,2′3′,4′,5′,6′-hexahydro-3′,4′-secoflava-4′,1′-lactone-3′-oic acid.28
The known compounds were identified by comparison of
spectroscopic data with those reported in the literature as (+)-
guaiacin (12),21 meso-dihydroguaiaretic acid (13),29 isomahubano-
lide-23 (14),11 hamabiwalactone A (15),10e ginkgolide A (16),7
ginkgolide B (17),7 4-(3-methoxy-4-hydroxy)pheny-3-methyl-3-
buten-2-one,30 nectandrin B,31 machilin-I,32 kobusin,33 eudesmin,33
2,3-(2R,3R)-dihydro-2-(4- hydroxy-3-methoxyphenyl)-7-methoxy-
3-methyl-5-(E)-propenylbenzofuran,34 (-)-epilitsenolides C2,35 lin-
comolide C,10d isolincomolide C,18a lincomolide B,9 4R-2-
tetradecylidene-4-methylolbutanolide,36 (4S)-2-(11-dodecenylidene)-
4-methylolbutanolide,36 aromadendrane-4ꢀ,10R-diol,37 and costic
acid.38
Extraction and Isolation. Air-dried bark of M. wangchiana (5.3
kg) was powdered and extracted with 95% EtOH (3 × 15 L) at room
temperature (3 × 48 h). The extract was evaporated under reduced
pressure to yield a dark brown residue (375 g). The residue was
suspended in H2O (1200 mL) and then partitioned with EtOAc (5 ×
1200 mL). After removing the solvent, the EtOAc extract (45 g) was
subjected to CC over silica gel, eluting with a gradient of increasing
MeOH (0-100%) in CHCl3 to afford 10 fractions (A1-A10) on the
basis of TLC analysis. Fraction A1 (6.5 g), eluted by CHCl3, was further
chromatographed over silica gel, eluting with a gradient of increasing
acetone (5-100%) in petroleum ether, to give 15 subfractions (A1-
1-A1-15). Fractions A1-2 (135 mg), A1-4 (1.2 g), A1-9 (87 mg), A1-
10 (91 mg), A1-11 (1.1 g), and A1-14 (282 mg) were chromatographed
separately over Sephadex LH-20 with petroleum ether-CHCl3-MeOH
(5:5:1) to afford subfractions A1-2a (21.9 mg), A1-9a (34.8 mg), A1-
11a (428.0 mg), and A1-14a (49.2 mg) and pure compounds 12 (522.0
mg) from A1-4 and 15 (11.4 mg) from A1-10. Subfraction A1-2a was
separated by RP-HPLC using 70% MeOH in H2O to yield 14 (6.7 mg).
Subfraction A1-9a was separated by RP- HPLC using 80% MeOH in
H2O to yield 3 (9.2 mg). Subfraction A1-11a was separated by RP-
HPLC using 50% CH3CN in H2O to give 1 (112 mg), 4 (36.1 mg), 5
(48.0 mg), and 7 (37.0 mg). Subfraction A1-14a was separated by RP-
HPLC using 55% CH3CN in H2O to yield 8 (1.7 mg) and 13 (51.7
mg). Fraction A2 (0.85 g), eluted by 1% MeOH in CHCl3, was
chromatographed over silica gel, eluting with a gradient of increasing
acetone (5-100%) in petroleum ether, to yield 13 fractions (A2-1-A2-
13). Fractions A2-3 (96 mg), A2-6 (224 mg), and A2-7 (152 mg) were
chromatographed separately over Sephadex LH-20 with petroleum
ether-CHCl3-MeOH (5:5:1) to afford a mixture of A2-3a (41 mg)
from A2-3 and pure compounds 2 (39 mg) and 9 (6.1 mg) from A2-7
and 16 (58 mg) and 17 (43 mg) from A2-6. The mixture A2-3a was
purified by RP-HPLC with 75% MeOH in H2O to yield 6 (7.3 mg).
Fraction A3 (0.49 g), eluted by 3% MeOH in CHCl3, was chromato-
graphed over silica gel, eluting with a gradient of increasing acetone
(7-100%) in petroleum ether, to give nine subfractions (A3-1-A3-
9). A3-3 (77 mg) was chromatographed over Sephadex LH-20 with
petroleum ether-CHCl3-MeOH (5:5:1) to afford A3-3a (38 mg), which
was further purified by RP-HPLC using 65% CH3CN in H2O to yield
10 (11.9 mg). Fraction A4 (2.8 g), eluted by 5% MeOH in CHCl3, was
chromatographed over silica gel, eluting with a gradient of increasing
MeOH (2-20%) in CHCl3, to give 10 subfractions (A4-1-A4-10).
A4-10 (43 mg) was chromatographed over Sephadex LH-20 using
CHCl3-MeOH (2:1) to afford 11 (8.4 mg).
In the in vitro bioassays, at 10-5 M, compounds 7, 8a, 8b, 9,
11, 12, 13, and 15 inhibited the release of ꢀ-glucuronidase in rat
polymorphonuclear leukocytes (PMNs) induced by platelet-activat-
ing factor (PAF) by 47%, 67%, 42%, 76%, 46%, 48%, 54%, and
60%, respectively [the positive control ginkgolide B (BN52021)39
gave a 76% inhibition]. At 10-4 M, compounds 8, 8a, 8b, 9, and
11 protected hepatocyte (WB-F344 cells) damage induced by DL-
galactosamine (GalN) with 43 ( 3%, 47 ( 2%, 54 ( 3%, 39 (
6%, and 40 ( 8% inhibitions (the positive control bicyclol40 showed
a 41 ( 2% inhibition). Compound 14 was cytotoxic to BGC-823
and A2780 cells with IC50 values of 0.13 and 2.66 µM, respectively
[positive control camptothecin (CPT), IC50 0.21 and 0.28 µM].
Experimental Section
General Experimental Procedures. Optical rotations were measured
on a PE model 343 polarimeter. UV spectra were measured on a Cary
300 spectrometer. CD spectra were recorded on a JASCO-815 CD
spectrometer. IR spectra were recorded on a Nicolet 5700 FT-IR
microscope instrument. 1D- and 2D-NMR spectra were obtained at 500
or 600 MHz for 1H and 125 or 150 MHz for 13C, respectively, on
INOVA 500 MHz or SYS 600 MHz spectrometers, in CDCl3 or
Me2CO-d6, with solvent peaks used as references. ESIMS data were
measured with a Q-Trap LC/MS/MS (Turbo Ionspray Source) spec-
trometer. ESIMS and HRESIMS data were measured using an Ac-
(+)-(2E,3R,4S)-2-(Dodec-11-ynylidene)-3-hydroxy-4-methylbu-
tanolide (1): colorless oil; [R]20 +26 (c 0.11, CHCl3); UV (MeOH)
D
λmax (log ε) 241 (2.98) nm; IR νmax 3341, 2923, 2117, 1738, 1678,
1459, 1376, 1319, 1109, 1053 cm-1; 1H NMR (CDCl3, 500 MHz) data,
see Table 1; 13C NMR (CDCl3, 125 MHz) data, see Table 2; ESIMS
m/z 279 [M + H]+, 301 [M + Na]+, and 317 [M + K]+; HRESIMS
m/z 301.1766 [M + Na]+ (calcd for C17H26O3Na, 301.1780).
Chemical Transformation of 1. A solution of 1 (21.0 mg) in EtOH
(2.5 mL) was hydrogenated (H2, 1 atm) over 10% Pd-C (40 mg) at

Chemical Constituents of Machilus wangchiana
Journal of Natural Products, 2009, Vol. 72, No. 12 2149
room temperature for 24 h. The reaction mixture was filtered, and then
the solvent was removed under reduced pressure to give 1a (20.2 mg):
amorphous powder; [R]20D -5 (c 0.08, CHCl3); 1H NMR (CDCl3, 600
MHz) δ 4.20 (1H, dq, J ) 6.0, 6.6 Hz, H-4), 3.84 (1H, t, J ) 6.0 Hz,
H-3), 2.55 (1H, dt, J ) 6.0, 7.8 Hz, H-2), 1.87 (1H, m, H-6a), 1.61
(1H, m, H-6b), 1.45 (3H, d, J ) 6.6 Hz, H3-5), 1.22-1.56 (m, H2-
7-H2-16), 0.88 (3H, t, J ) 7.2 Hz, H3-17). A solution of 1a (4.5 mg)
in pyridine (1 mL) and Ac2O (5 µL) was kept at room temperature for
24 h. After the solvent was removed under reduced pressure, the residue
was partitioned between a saturated CuSO4 water solution (10 mL)
and EtOAc (10 mL). The EtOAc phase was washed with water and
then evaporated under reduced pressure to yield 1b (4.0 mg): colorless
λmax (log ε) 241 (2.91) nm; IR νmax 3402, 2922, 1737, 1678, 1462,
1
1377, 1196, 1049 cm-1; H NMR (CDCl3, 500 MHz) data, see Table
1; 13C NMR (CDCl3, 125 MHz) data, see Table 2; ESIMS m/z 281 [M
+ H]+, 303 [M + Na]+; HRESIMS m/z 303.1937 [M + Na]+ (calcd
for C17H28O3Na, 303.1931).
(+)-(2Z,3R,4S)-2-(Dodec-11-ynylidene)-3-hydroxy-4-methylbu-
tanolide (4): colorless oil; [R]20 +13 (c 0.11, CHCl3); UV (MeOH)
D
λmax (log ε) 241 (3.37) nm; IR νmax 3340, 2923, 1736, 1669, 1427,
1
1372, 1107, 1054 cm-1; H NMR (CDCl3, 500 MHz) data, see Table
1; 13C NMR (CDCl3, 125 MHz) data, see Table 2; ESIMS m/z 279 [M
+ H]+, 301 [M + Na]+; HRESIMS m/z 301.1784 [M + Na]+ (calcd
for C17H26O3Na, 301.1774).
1
gum; H NMR (CDCl3, 600 MHz) δ 4.92 (1H, t, J ) 4.8 Hz, H-3),
(-)-(2Z,3S,4S)-2-(Dodec-11-ynylidene)-3-hydroxy-4-methylbu-
tanolide (5): colorless oil; [R]20 -26 (c 0.10, CHCl3); UV (MeOH)
4.38 (1H, dq, J ) 4.8, 6.6 Hz, H-4), 2.68 (1H, m, H-2), 2.10 (3H, s,
OAc), 1.46 (3H, d, J ) 6.6 Hz, H3-5), 1.20-1.89 (m, H2-6-H2-16),
0.88 (3H, t, J ) 6.6 Hz, H3-17); FABMS m/z 327 [M + H]+. To a
anhydrous THF (1 mL) solution of 1b (4.0 mg) was added DBU (1,8-
diazabicyclo[5.4.0]undec-7-ene, 3 µL). The mixture was stirred at room
temperature for 1 h and then neutralized with several drops of HOAc
and concentrated under reduced pressure to give a residue. The residue
was chromatographed over silica gel eluting with CHCl3 to give 1c
D
λ
max (log ε) 241 (2.8) nm; IR νmax 3310, 2919, 1736, 1674, 1461, 1376,
1
1182, 1048 cm-1; H NMR (CDCl3, 500 MHz) data, see Table 1; 13C
NMR (CDCl3, 125 MHz) data, see Table 2; ESIMS m/z 279 [M +
H]+, 301 [M + Na]+; HRESIMS m/z 301.1787 [M + Na]+ (calcd for
C17H26O3Na, 301.1774).
Chemical Transformation of 2-5. A solution of each compound
in EtOH (2.0 mL) was hydrogenated (H2, 1 atm) over 10% Pd-C (40
mg) at room temperature for 24 h. The reaction mixture was filtered,
and the filtrate was evaporated under reduced pressure. Pyridine (1.0
mL) and Ac2O (5 µL) were added to the residue and kept at room
temperature overnight. After the solvent was removed under reduced
pressure, the residue was partitioned between a saturated CuSO4 water
solution (10 mL) and EtOAc (10 mL). Each EtOAc extract was washed
by water and evaporated under reduced pressure. After anhydrous THF
(1 mL) and DBU (5 µL) were added to the residue, the solution was
kept at room temperature for 1 h and then neutralized with several
drops of HOAc followed by concentrating under reduced pressure. The
residue was separated by TLC with CHCl3 as a developing solvent to
yield 1c. The 1H NMR, EIMS, and specific rotation data of 1c, obtained
from the transformation of 2-5, were identical to those of [(+)-(5S)-
3-dodecyl-5-methylfuran-2(5H)-one14].
[(+)-(5S)-3-dodecyl-5-methylfuran-2(5H)-one,14 2.7 mg]: white pow-
1
der; [R]20 +27 (c 0.12, CHCl3); H NMR (CDCl3, 600 MHz) δ 6.98
D
(1H, brs, H-3), 4.99 (1H, brq, J ) 6.6 Hz, H-4), 2.26 (2H, t, J ) 7.8
Hz, H2-6), 1.40 (3H, d, J ) 6.6 Hz, H3-5), 1.20-1.59 (m, H2-7-H2-
16), 0.88 (3H, t, J ) 6.6 Hz, H3-17); EIMS m/z 266 [M]+•. 1 (5 mg)
and 4-dimethylaminopyridine (DMAP, 1.2 mg) were dissolved in
anhydrous pyridine (1 mL) at 0 °C, and then the solution was kept at
room temperature for 5 h. After removing the solvent under reduced
pressure, the residue was partitioned between a saturated CuSO4 water
solution (10 mL) and EtOAc (10 mL). The EtOAc phase was evaporated
under reduced pressure to give a residue that was separated by
preparative TLC using 15% Me2CO in petroleum ether as developing
solvent to yield 1d [(E)-hexadeca-3-ene-15-ynyl-2,5-dione]: colorless
1
oil; H NMR (CDCl3, 500 MHz) δ 6.83 (2H, s, H-3, H-4), 2.64 (2H,
ent-Litsenolide C1 (6): colorless oil; [R]20 +30 (c 0.09, CHCl3);
t, J ) 7.5 Hz, H2-6), 2.37 (3H, s, H3-1), 2.18 (2H, dt, J ) 2.5, 7.0 Hz,
H2-14), 1.93 (1H, t, J ) 2.5 Hz, H-16), 1.24-1.70 (m, H2-7-H2-13);
13C NMR (CDCl3, 125 MHz) 198.5 and 200.6 (C-2, C-5), 136.9 and
137.2 (C-3, C-4), 84.7 (C-15), 68.1 (C-16), 41.4 (C-6), 29.3 (C-1),
23.7-29.1 (C-7-C-13), δ 18.4 (C-14); ESIMS m/z 249 [M + H]+,
271 [M + Na]+.
D
UV (MeOH) λmax (log ε) 249 (2.6) nm; IR νmax 3387, 2920, 1730, 1462,
1
1378, 1216, 1034, 991 cm-1; H NMR (CDCl3, 500 MHz) data, see
Table 1; 13C NMR (CDCl3, 125 MHz) data, see Table 2; ESIMS m/z
311 [M + H]+, 333 [M + Na]+; HRESIMS m/z 333.2370 [M + Na]+
(calcd for C19H34O3Na, 333.2400).
Preparation of MTPA Esters of 1a. 1a (6.0 mg) and DMAP (4-
dimethylaminopyridine, 1.5 mg) were dissolved in anhydrous pyridine
(1.5 mL) at 0 °C, and then (R)-(-)-R-methoxy-R-(trifluoromethyl)phe-
nylacetyl chloride [(R)-(-)-MTPACl, 10 µL] was added to the solution.
After stirring at room temperature for 5 h, the reaction mixture was
evaporated under reduced pressure to give a residue, which was
partitioned between a saturated CuSO4 water solution (10 mL) and
EtOAc (10 mL). The EtOAc extract was evaporated under reduced
pressure to give a residue that was purified by preparative TLC using
15% Me2CO in petroleum ether as developing solvent to give S-MTPA-
1a (5.6 mg). R-MTPA-1a (4.9 mg) was prepared by using the same
procedure with 1a (5.1 mg) and (S)-(+)-MTPACl (10 µL) as starting
material and reagent, respectively. S-MTPA-1a: colorless gum; 1H NMR
(CDCl3, 600 MHz) δ 7.4-7.5 (5H, m, aromatic protons of MTPA
moiety), 5.12 (1H, t, J ) 6.0 Hz, H-3), 4.32 (1H, dq, J ) 6.0, 6.6 Hz,
H-4), 3.52 (3H, s, MeO of MTPA moiety), 2.75 (1H, dt, J ) 6.0, 7.8
Hz, H-2), 1.88 (1H, m, H-6a), 1.66 (1H, m, H-6b), 1.47 (3H, d, J )
6.6 Hz, H3-5), 1.2-1.6 (m, H2-7-H2-16), 0.88 (3H, t, J ) 7.2 Hz,
(-)-(7′R,8R,8′R)-4,4′-Dihydroxy-3,3′,5-trimethoxy-2,7′-cyclolig-
nane (7): amorphous powder; [R]20D -13 (c 0.03, CHCl3); UV (MeOH)
λmax (log ε) 203 (4.72), 231 (4.15), 281 (3.71) nm; CD (MeOH) 289
(∆ε +0.16), 274.5 (∆ε -1.87) nm; IR νmax 3366, 2949, 1611, 1502,
1460, 1271, 1225, 1121, 1102, 1037 cm-1; 1H NMR (CDCl3, 500 MHz)
δ 6.73 (1H, d, J ) 8.0 Hz, H-5′), 6.61 (1H, d, J ) 1.5 Hz, H-2′), 6.44
(1H, s, H-6), 6.37 (1H, dd, J ) 8.0, 1.5 Hz, H-6′), 4.05 (1H, d, J ) 2.0
Hz, H-7′), 3.89 (3H, s, MeO-5), 3.83 (3H, s, MeO-3′), 3.36 (3H, s,
MeO-3), 2.70 (1H, dd, J ) 16.5, 6.0 Hz, H-7a), 2.39 (1H, dd, J )
16.5, 11.5 Hz, H-7b), 2.02 (1H, m, H-8), 1.85 (1H, m, H-8′), 0.91 (3H,
d, J ) 7.5 Hz, H3-9′), 0.88 (3H, d, J ) 7.0 Hz, H3-9); 13C NMR (CDCl3,
125 MHz) δ 146.2 (C-5), 146.1 (C-3′), 145.6 (C-3), 143.4 (C-4′), 140.0
(C-1′), 136.7 (C-4), 128.0 (C-2), 123.5 (C-1), 121.2 (C-6′), 113.4 (C-
5′), 111.2 (C-2′), 105.9 (C-6), 59.8 (MeO-3), 56.0 (MeO-5), 55.9 (MeO-
3′), 46.9 (C-7′), 40.7 (C-8′), 33.4 (C-7), 25.9 (C-8), 18.8 (C-9), 13.7
(C-9′); ESIMS m/z 397 [M + K]+; HRESIMS m/z 397.1436 [M +
K]+ (calcd for C21H26O5K, 397.1417).
(+)-(7S,8R)-4-Hydroxy-3-methoxy-1′,2′,3′,4′,5′,6′,7′-heptanorlign-
1
8′-one (8): white, amorphous powder; [R]20 +15 (c 0.05, CHCl3);
H3-17). R-MTPA-1a: colorless gum; H NMR (CDCl3, 600 MHz) δ
D
7.4-7.5 (5H, m, aromatic protons of MTPA moiety), 5.11 (1H, t, J )
6.0 Hz, H-3), 4.43 (1H, dq, J ) 6.0, 6.0 Hz, H-4), 3.54 (3H, s, MeO
of MTPA moiety), 2.67 (1H, dt, J ) 6.0, 7.2 Hz, H-2), 1.83 (1H, m,
H-6a), 1.61 (1H, m, H-6b), 1.51 (3H, d, J ) 6.0 Hz, H3-5), 1.2-1.6
(m, H2-7-H2-16), 0.88 (3H, t, J ) 7.2 Hz, H3-17).
UV (MeOH) λmax (log ε) 230 (4.29), 280 (3.92) nm; IR νmax 3291,
1
2922, 1737, 1462, 1377, 1191, 1085, 1021 cm-1; H NMR (CDCl3,
500 MHz) δ 6.88 (1H, d, J ) 1.5 Hz, H-2), 6.88 (1H, d, J ) 8.0 Hz,
H-5), 6.80 (1H, dd, J ) 8.0, 1.5 Hz, H-6), 4.68 (1H, d, J ) 9.0 Hz,
H-7), 3.91 (3H, s, MeO-3), 2.89 (1H, dq, J ) 9.0, 7.5 Hz, H-8), 2.24
(3H, s, H3-9′), 0.93 (3H, d, J ) 7.5 Hz, H3-9); 13C NMR (CDCl3, 125
MHz) δ 213.4 (C-8′), 146.3 (C-3), 145.4 (C-4), 134.0 (C-1), 119.9
(C-6), 114.1 (C-5), 108.7 (C-2), 76.5 (C-7), 56.0 (MeO-3), 53.9 (C-8),
29.7 (C-9′), 14.2 (C-9); ESIMS m/z 247 [M + Na]+; HRESIMS m/z
247.0923 [M + Na]+ (calcd for C12H16O4Na, 247.0941).
(+)-(7S,8R)-4-Hydroxy-3,7-dimethoxy-1′,2′,3′,4′,5′,6′,7′-heptan-
orlign-8′-one (8a): colorless gum; [R]20D +11 (c 0.03, CHCl3); 1H NMR
(CDCl3, 500 MHz) δ 6.89 (1H, d, J ) 8.5 Hz, H-5), 6.82 (1H, d, J )
1.5 Hz, H-2), 6.78 (1H, dd, J ) 8.5, 1.5 Hz, H-6), 4.11 (1H, d, J )
(+)-(2E,3R,4S)-2-(Dodec-11-enylidene)-3-hydroxy-4-methylbu-
tanolide (2): colorless oil; [R]20 +16 (c 0.12, CHCl3); UV (MeOH)
D
λmax (log ε) 242 (2.86) nm; IR νmax 3421, 2919, 1738, 1678, 1461,
1
1377, 1201, 1027 cm-1; H NMR (CDCl3, 500 MHz) data, see Table
1; 13C NMR (CDCl3, 125 MHz) data, see Table 2; ESIMS m/z 281 [M
+ H]+, 303 [M + Na]+, 319 [M + K]+; HRESIMS m/z 303.1950 [M
+ Na]+ (calcd for C17H28O3Na, 303.1936).
(+)-(2Z,3R,4S)-2-(Dodec-11-enylidene)-3-hydroxy-4-methylbu-
tanolide (3): colorless oil; [R]20 +2 (c 0.09, CHCl3); UV (MeOH)
D

2150 Journal of Natural Products, 2009, Vol. 72, No. 12
Cheng et al.
10.0 Hz, H-7), 3.91 (3H, s, MeO-3), 3.10 (3H, s, MeO-7), 2.84 (1H,
dq, J ) 10.0, 7.5 Hz, H-8), 2.26 (3H, s, H3-9′), 0.77 (3H, d, J ) 7.5
Hz, H3-9); ESIMS m/z 261 [M + Na]+; HRESIMS m/z 261.1098 [M
+ Na]+ (calcd for C13H18O4Na, 261.1097).
(CDCl3, 600 MHz) δ 6.22 (1H, s, H-13a), 5.64 (1H, s, H-13b), 3.78
(3H, s, MeO), 3.01 (1H, m, H-10), 2.74 (1H, dq, J ) 6.6, 7.2 Hz,
H-4), 2.61 (1H, dd, J ) 18.6, 6.6 Hz, H-3a), 2.60 (1H, m, H-6a), 2.59
(1H, m, H-7), 2.42 (1H, m, H-6b), 1.98 (1H, d, J ) 18.6 Hz, H-3b),
1.92 (1H, m, H-8a), 1.82 (1H, m, H-8b), 1.78 (1H, m, H-9a), 1.58
(1H, m, H-9b), 1.11 (3H, d, J ) 7.2 Hz, H3-14), 1.02 (3H, d, J ) 7.2
Hz, H3-15). 10a (2.3 mg) was hydrogenated in EtOH (1 mL) over 10%
Pd-C (2.5 mg) at room temperature for 24 h. The reaction mixture
was filtered, and the solvent was removed under reduced pressure to
give a mixture of two isomers. The mixture was further reduced with
NaBH4 (0.3 mg) in anhydrous EtOH (1 mL) at room temperature for
3 h. The solvent was removed, and the residue was chromatographed
on silica gel eluting with CHCl3 to afford another mixture, of which
the 1H NMR spectrum indicated that it contained 10b and 10c
(Supporting Infromation), which we were unable to separate. The
mixture was dissolved in anhydrous pyridine (1 mL), and the solution
was divided into two portions, which were treated with S- and
R-MTPACl at room temperature for 4 h, respectively. After pyridine
was evaporated from the reaction solutions, by using a reversed-phase
semipreparative HPLC separation with 85% CH3CN in H2O as mobile
phase, 10bR (0.5 mg) and 10cR (0.5 mg) were obtained from the portion
reacted with S-MTPACl, while 10bS (0.3 mg) and 10cS (0.3 mg) were
isolated from the portion reacted with R-MTPACl. 10bR: colorless gum;
1H NMR (CDCl3, 500 MHz) δ 7.3-7.5 (5H, m, aromatic protons of
MTPA moiety), 5.36 (1H, m, H-2), 3.64 (3H, s, MeO-12), 3.52 (3H, s,
MeO of MTPA moiety), 2.49 (1H, dt, J ) 14.5, 8.0 Hz, H-3a), 2.23
(1H, m, H-11), 2.01 (1H, m, H-1), 1.95 (1H, m, H-4), 1.88 (1H, m,
H-10), 1.86 (1H, m, H-7), 1.77 (1H, m, H-5), 1.65 (1H, m, H-9a), 1.40
(1H, m, H-8a), 1.26 (1H, m, H-8b), 1.06 (2H, m, H-3b, H-9b), 1.05
(1H, m, H-6a), 0.99 (1H, dt, J ) 11.5, 12.5 Hz, H-6b), 0.94 (3H, d, J
) 7.0 Hz, H3-13), 0.91 (3H, d, J ) 7.5 Hz, H3-15), 0.84 (3H, d, J )
(+)-(7S,8R)-7-Ethoxy-4-hydroxy-3-methoxy-1′,2′,3′,4′,5′,6′,7′-hep-
tanorlign-8′-one (8b): colorless gum; [R]20D +13 (c 0.05, CHCl3); 1H
NMR (CDCl3, 500 MHz) δ 6.87 (1H, d, J ) 8.0 Hz, H-5), 6.83 (1H,
d, J ) 1.5 Hz, H-2), 6.77 (1H, dd, J ) 8.0, 1.5 Hz, H-6), 4.19 (1H, d,
J ) 9.5 Hz, H-7), 3.91 (3H, s, MeO-3), 3.29 (1H, dq, J ) 9.5, 7.0 Hz,
H-1′′a), 3.20 (1H, dq, J ) 9.5, 7.0 Hz, H-1′′b), 2.84 (1H, dq, J ) 9.5,
7.5 Hz, H-8), 2.27 (3H, s, H3-9′), 1.07 (3H, t, J ) 7.0 Hz, H3-2′′), 0.76
(3H, d, J ) 7.5 Hz, H3-9); ESIMS m/z 275 [M + Na]+; HRESIMS
m/z 275.1253 [M + Na]+ (calcd for C14H20O4Na, 275.1254).
(+)-(7S,8R,8′R)-4,8′-Dihydroxy-3-methoxy-1′,2′,3′,4′,5′,6′-hexan-
orligna-7′,7-lactone (9): amorphous powder; [R]20 +14 (c 0.05,
D
CHCl3); UV (MeOH) λmax (log ε) 203 (4.20), 231 (2.90), 280 (2.46)
nm; CD (MeOH) 235 (∆ε -0.96), 279 (∆ε -0.03) nm; IR νmax 3437,
2921, 1768, 1611, 1515, 1273, 1240, 1030, 951, 937, 847, 821, 759
1
cm-1; H NMR (CDCl3, 500 MHz) δ 6.92 (1H, d, J ) 8.5 Hz, H-5),
6.82 (1H, d, J ) 1.5 Hz, H-2), 6.82 (1H, dd, J ) 8.5, 1.5 Hz, H-6),
5.07 (1H, d, J ) 9.5 Hz, H-7), 3.91 (3H, s, MeO-3) 2.04 (1H, dq, J )
9.5, 6.5 Hz, H-8), 1.50 (3H, s, H3-9′), 1.08 (3H, d, J ) 6.5 Hz, H3-9);
13C NMR (CDCl3, 125 MHz) δ 177.5 (C-7′), 146.8 (C-3), 146.2 (C-
4), 128.6 (C-1), 119.9 (C-6), 114.3 (C-5), 108.5 (C-2), 85.5 (C-7), 74.7
(C-8′), 56.1 (MeO-3), 49.6 (C-8), 22.0 (C-9′), 7.6 (C-9); ESIMS m/z
253 [M + H]+ and 275 [M + Na]+; HRESIMS m/z 253.1071 [M +
H]+ (calcd for C13H17O5, 253.1076).
Chemical Transformation of 9. To a solution of 9 (5.0 mg) in dry
THF (1.0 mL) was added a suspension of LiAlH4 (2.5 mg) in dry THF
(0.5 mL). The reaction mixture was stirred at room temperature for
5 h. After workup, the solution was neutralized with 10% HCl to pH
4 and then partitioned between H2O (30 mL) and EtOAc (30 mL). The
EtOAc phase was evaporated under reduced pressure to give a residue
that was separated by preparative TLC using 50% Me2CO in petroleum
1
7.0 Hz, H3-14). 10cR: colorless gum; H NMR (CDCl3, 500 MHz) δ
7.3-7.5 (5H, m, aromatic protons of MTPA moiety), 5.33 (1H, m,
H-2), 3.64 (3H, s, MeO-12), 3.54 (3H, s, MeO of MTPA moiety), 2.49
(1H, dt, J ) 14.0, 8.0 Hz, H-3a), 2.15 (1H, m, H-11), 2.00 (1H, m,
H-1), 1.94 (1H, m, H-4), 1.89 (1H, m, H-10), 1.81 (1H, m, H-7), 1.73
(1H, m, H-5), 1.63 (1H, m, H-9a), 1.47 (1H, m, H-8a), 1.28 (1H, m,
H-8b), 1.24 (1H, m, H-6a), 1.08 (1H, m, H-9b), 1.05 (1H, m, H-3b),
0.92 (3H, d, J ) 7.0 Hz, H3-13), 0.92 (3H, d, J ) 7.0 Hz, H3-15), 0.85
(3H, d, J ) 7.0 Hz, H3-14), 0.77 (1H, dt, J ) 11.0, 13.0 Hz, H-6b).
10bS: colorless gum; 1H NMR (CDCl3, 500 MHz) δ 7.3-7.5 (5H, m,
aromatic protons of MTPA moiety), 5.33 (1H, m, H-2), 3.66 (3H, s,
MeO-12), 3.63 (3H, s, MeO of MTPA moiety), 2.53 (1H, dt, J ) 15.0,
8.0 Hz, H-3a), 2.33 (1H, m, H-11), 1.97 (2H, m, H-1, H-4), 1.88 (1H,
m, H-7), 1.81 (1H, m, H-5), 1.76 (1H, m, H-10), 1.2-1.5 (6H, m, H-3b,
H2-6, H2-8, and H-9a), 1.07 (3H, d, J ) 7.0 Hz, H3-13), 0.95 (3H, d,
J ) 6.5 Hz, H3-14), 0.78 (1H, m, H-9b), 0.61 (3H, d, J ) 7.0 Hz,
1
ether as developing solvent to yield 9a (3.0 mg): colorless gum; H
NMR (CDCl3, 600 MHz) δ 6.88 (1H, d, J ) 7.8 Hz, H-5), 6.86 (1H,
d, J ) 1.8 Hz, H-2), 6.81 (1H, dd, J ) 7.8, 1.8 Hz, H-6), 4.55 (1H, d,
J ) 10.8 Hz, H-7), 4.02 (1H, d, J ) 9.6 Hz, H-7′a), 3.95 (1H, d, J )
9.6 Hz, H-7′b), 3.91 (3H, s, MeO-3), 1.79 (1H, dq, J ) 10.8, 6.6 Hz,
H-8), 1.35 (3H, s, H3-9′), 0.96 (3H, d, J ) 6.6 Hz, H3-9); ESIMS m/z
255 [M - H]-. To a solution of 9a (3 mg) in THF (0.7 mL)-H2O (0.2
mL) was added dropwise a solution of NaIO4 (5 mg) in water (0.1
mL). After stirring at room temperature overnight, the reaction mixture
was diluted with H2O (30 mL) and then partitioned with EtOAc (30
mL). The EtOAc phase was evaporated under reduced pressure to give
a residue, which was separated by preparative TLC using 4% MeOH
in CHCl3 to yield 8 (2.4 mg): white, amorphous powder; [R]20 +23
D
1
(c 0.09, CHCl3); 1H NMR (CDCl3, 600 MHz) and ESIMS data, identical
to those of the natural product.
H3-15). 10cS: colorless gum; H NMR (CDCl3, 500 MHz) δ 7.3-7.5
(5H, m, aromatic protons of MTPA moiety), 5.33 (1H, m, H-2), 3.66
(3H, s, MeO-12), 3.62 (3H, s, MeO of MTPA moiety), 2.53 (1H, dt, J
) 15.0, 8.0 Hz, H-3a), 2.31 (1H, m, H-11), 1.98 (2H, m, H-1, H-4),
1.88 (1H, m, H-7), 1.78 (2H, m, H-5, H-10), 1.2-1.5 (6H, m, H-3b,
H2-6, H2-8, and H-9a), 1.08 (3H, d, J ) 7.0 Hz, H3-13), 0.96 (3H, d,
J ) 7.0 Hz, H3-14), 0.85 (1H, m, H-9b), 0.64 (3H, d, J ) 7.5 Hz,
H3-15).
(-)-(4S,7S,10S)-2-Oxo-guaia-1(5),11(13)-dien-12-oic acid (10):
colorless needles; [R]20D -16 (c 0.05, MeOH); UV (MeOH) λmax (log
ε) 202 (3.99), 240 (4.06) nm; CD (MeOH) 218 (∆ε -2.21), 236 (∆ε
+0.63), 244 (∆ε -0.07), 249 (∆ε +0.06), 256 (∆ε -0.33), 268 (∆ε
+0.04), 301 (∆ε -0.21) nm; IR νmax 2925, 1695, 1639, 1391, 1274,
1224, 1164 cm-1; 1H NMR (CDCl3, 500 MHz) δ 6.38 (1H, s, H-13a),
5.76 (1H, s, H-13b), 3.01 (1H, m, H-10), 2.75 (1H, dq, J ) 6.5, 7.5
Hz, H-4), 2.61 (1H, dd, J ) 18.5, 6.5 Hz, H-3a), 2.61 (1H, m, H-7),
2.60 (1H, m, H-6a), 2.44 (1H, m, H-6b), 1.98 (1H, d, J ) 18.5 Hz,
H-3b), 1.93 (1H, m, H-8a), 1.85 (1H, m, H-8b), 1.80 (1H, m, H-9a),
1.59 (1H, m, H-9b), 1.11 (3H, d, J ) 7.5 Hz, H3-14), 1.03 (3H, d, J )
7.0 Hz, H3-15); 13C NMR (CDCl3, 125 MHz) δ 208.1 (C-2), 175.9
(C-5), 170.6 (C-12), 145.7 (C-1), 145.4 (C-11), 125.5 (C-13), 43.0 (C-
3), 39.3 (C-7), 37.8 (C-4), 37.0 (C-6), 32.6 (C-9), 30.9 (C-8), 26.7
(C-10), 19.1 (C-14), 17.5 (C-15); ESIMS m/z 249 [M + H]+, 271 [M
+ Na]+; HRESIMS m/z 249.1512 [M + H]+ (calcd for C15H21O3,
249.1491).
Preparation of 10a and MTPA Esters of 10b and 10c. Compound
10 (3.1 mg), DMAP (4-dimethylaminopyridine, 1.5 mg), and EDCI
[1-ethyl-3-(3-dimethyllaminopropyl)carbodiimide hydrochloride, 4.8
mg) were dissolved in anhydrous CH2Cl2 (1 mL). After the solution
was cooled at 0 °C, anhydrous MeOH (2 µL) was added. The reaction
mixture was stirred at room temperature for 1.5 h, and then the solvent
was removed. The residue was chromatographed over silica gel (0.5
g) eluting with CHCl3 to yield 10a (2.3 mg): colorless gum; 1H NMR
(+)-(2S,3R,1′S,6′R)-5,7-Dihydroxy-3,6′-epoxy-1′,2′,3′,4′,5′,6′-tet-
rahydro-3′,4′-secoflava-4′,1′-lactone-3′-oic acid (11): colorless gum;
[R]20D +39 (c 0.06, MeOH); UV (MeOH) λmax (log ε) 204 (4.18), 230
(3.48), 277 (2.92) nm; CD (MeOH) 218 (∆ε +0.70), 227 (∆ε +0.65),
277 (∆ε -0.04) nm; IR νmax 3359, 2922, 1786, 1728, 1627, 1522, 1468,
1398, 1153, 1047 cm-1; 1H NMR (acetone-d6, 400 MHz) δ 6.05 (1H,
d, J ) 2.0 Hz, H-6), 5.91 (1H, d, J ) 2.0 Hz, H-8), 4.78 (1H, d, J )
6.8 Hz, H-6′), 4.52 (1H, dt, J ) 4.8, 1.6 Hz, H-3), 4.45 (1H, d, J ) 1.6
Hz, H-2), 3.20 (2H, s, H2-2′), 3.19 (1H, dd, J ) 18.4, 6.8 Hz, H-5′a),
2.91 (1H, dd, J ) 18.0, 1.6 Hz, H-4a), 2.76 (1H, dd, J ) 18.0, 4.8 Hz,
H-4b), 2.50 (1H, d, J ) 18.4 Hz, H-5′b); 13C NMR (acetone-d6, 125
MHz) δ 176.0 (C-4′), 172.0 (C-3′), 157.5 (C-7), 157.0 (C-5), 154.6
(C-8a), 98.6 (C-4a), 96.6 (C-6), 95.8 (C-8), 94.3 (C-1′), 80.8 (C-6′),
79.3 (C-2), 72.6 (C-3), 37.5 (C-5′), 36.9 (C-2′), 21.1 (C-4); ESIMS
m/z 321 [M - H]-, 345 [M + Na]+; HRESIMS m/z 345.0627 [M +
Na]+ (calcd for C15H14O8Na, 345.0586).
Methylation of 11. A solution of 11 (1.5 mg) in dry acetone (1
mL) was treated with K2CO3 (1.5 mg) and CH3I (2 mg) at 40 °C for
8 h. The reaction mixture was then evaporated under reduced pressure

Chemical Constituents of Machilus wangchiana
Journal of Natural Products, 2009, Vol. 72, No. 12 2151
(2) Yu, Y. U.; Kang, S. Y.; Park, H. Y.; Sung, S. H.; Lee, E. J.; Kim,
to give a residue. The residue was partitioned between H2O (10 mL)
and EtOAc (10 mL). After removing solvent, the EtOAc extract was
purified by RP-HPLC using a mobile phase of CH3CN-H2O (67:33)
to afford 11a (0.8 mg): white powder; IR νmax 2965, 2948, 1794, 1742,
1621, 1597, 1502, 1465, 1431, 1371, 1219, 1149, 1058, 838 cm-1; 1H
NMR (acetone-d6, 600 MHz) δ 6.15 (1H, d, J ) 2.4 Hz, H-6), 6.06
(1H, d, J ) 2.4 Hz, H-8), 4.76 (1H, d, J ) 6.6 Hz, H-6′), 4.54 (1H,
ddd, J ) 4.8, 2.4, 1.8 Hz, H-3), 4.50 (1H, d, J ) 2.4 Hz, H-2), 3.79
(3H, s, MeO-5), 3.73 (3H, s, MeO-7), 3.69 (3H, s, MeO-3′), 3.27 (1H,
d, J ) 17.4 Hz, H-2′a), 3.23 (1H, d, J ) 17.4 Hz, H-2′b), 3.17 (1H,
dd, J ) 18.6, 7.2 Hz, H-5′a), 2.89 (1H, dd, J ) 18.0, 1.8 Hz, H-4a),
2.75 (1H, dd, J ) 18.0, 4.8 Hz, H-4b), 2.53 (1H, d, J ) 18.6 Hz,
H-5′b); 13C NMR (acetone-d6, 125 MHz) δ 175.7 (C-4′), 170.8 (C-3′),
160.5 (C-7), 159.4 (C-5), 154.1 (C-8a), 105.5 (C-4a), 94.3 (C-6), 93.9
(C-1′), 92.6 (C-8), 80.6 (C-6′), 79.0 (C-2), 72.4 (C-3), 55.8 (MeO-7),
55.5 (MeO-5), 52.0 (MeO-3′), 37.2 (C-5′), 36.6 (C-2′), 21.0 (C-4);
HRESIMS m/z 387.1064 [M + Na]+ (calcd for C18H20O8Na, 387.1050).
Anti-inflammatory Activity Assay. See ref 39.
S. Y.; Kim, Y. C. J. Pharm. Pharmacol. 2000, 52, 1163–1169.
(3) Cheng, M. J.; Jayaprakasam, B.; Ishikawa, T.; Seki, H.; Tsai, I. L.;
Wang, J. J.; Chen, I. S. HelV. Chim. Acta 2002, 85, 1909–1914.
(4) (a) Talapatra, B.; Goswami, S.; Ghash, A.; Talapetra, S. K. J. Indian
Chem. Soc. 1982, 59, 1364–1368. (b) Liu, M. T.; Lin, S.; Wang, Y. H.;
He, W. Y.; Li, S.; Wang, S. J.; Yang, Y. C.; Shi, J. G. Org. Lett.
2007, 9, 129–132.
(5) (a) Kim, S. H.; Park, J. C. Sikmul Hakhoechi 1993, 36, 297–300. (b)
Lee, J. S.; Kim, J.; Yu, Y. U.; Kim, Y. C. Arch. Pharm. Res. 2004,
27, 1043–1047.
(6) Gan, M. L.; Zhang, Y. L.; Lin, S.; Liu, M. T.; Song, W. X.; Zi, J. C.;
Yang, Y. C.; Fan, X. N.; Shi, J. G.; Hu, J. F.; Sun, J. D.; Chen, N. H.
J. Nat. Prod. 2008, 71, 647–654, and references therein.
(7) Van Beek, T. A.; Lankhorst, P. P. Tetrahedron 1996, 52, 4505–4514.
(8) (a) Takeda, K. I.; Sakurawi, K.; Ishii, H. Tetrahedron 1972, 28, 3757–
3766. (b) Chen, I. S.; Lai-Yaun, I. L.; Duh, C. Y.; Tsai, I. L.
Phytochemistry 1998, 49, 745–750.
(9) Tsai, I. L.; Hung, C. H.; Duh, C. Y.; Chen, J. H.; Lin, W. Y.; Chen,
I. S. Planta Med. 2001, 67, 865–866.
Cells, Culture Conditions, and Cell Proliferation Assay. See ref
41.
(10) (a) Tanaka, H.; Nakamura, T.; Ichino, K.; Ito, K.; Tanaka, T.
Phytochemistry 1990, 29, 857–859. (b) Pupo, M. T.; Vieira, P. C.;
Fernandes, J. B.; Da Silva, M. F. D. G. F. Phytochemistry 1998, 48,
307–310. (c) Claros, B. M.; da Silva, A. J.; Vasconcellos, M. L.; de
Brito, A. P.; Leita˜o, G. G. Phytochemistry 2000, 55, 859–862. (d)
Tsai, I. L.; Hung, C. H.; Duh, C. Y.; Chen, I. S. Planta Med. 2002,
68, 142–145. (e) Min, B. S.; Lee, S. Y.; Kim, J. H.; Kwon, O. K.;
Park, B. Y.; An, R. B.; Lee, J. K.; Moon, H. I.; Kim, T. J.; Kim,
Y. H.; Joung, H.; Lee, H. K. J. Nat. Prod. 2003, 66, 1388–1390.
(11) Juan, C.; Martinez, V.; Yoshida, M.; Gottlieb, O. R. Phytochemistry
1981, 20, 459–464.
(12) (a) Qian, Y.; Yao, Z. J.; Chen, X. G.; Wu, Y. L. J. Org. Chem. 1999,
64, 2440–2445. (b) Lee, S. S.; Chang, S. M.; Chen, C. H. J. Nat.
Prod. 2001, 64, 1548–1551.
(13) Omata, K.; Fujiwara, T.; Kabuto, K. Tetrahedron: Asymmetry 2002,
13, 1655–1662.
Protective Effect on Cytotoxicity Induced by DL-Galactosamine
in WB-F344 Cells. The hepatoprotective effects were determined by
a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)
colorimetric assay42 in WB-F344 cells, with some modification. Each
cell suspension of 1 × 104 cells in 200 µL of Dulbecco’s modified
Eagle’s medium (DMEM) containing fetal calf serum (10%), penicillin
(100 units/mL), and streptomycin (100 µg/mL) was plated in a 96-
well microplate and precultured for 24 h at 37 °C under a 5% CO2
atmosphere. After fresh medium (200 µL) containing bicyclol (the
positive control) or test sample was added, the cells were cultured for
1 h. Then, the cultured cells were exposed to 50 mM DL-galactosamine
for 24 h. Cytotoxic effects of test samples were measured simulta-
neously in the absence of DL-galactosamine. The medium was changed
into a fresh one containing 0.5 mg/mL MTT. After 4 h incubation, the
medium was removed and 150 µL of DMSO was added to dissolve
formazan crystals. The optical density (OD) of the formazan solution
was measured on a microplate reader at 490 nm. Inhibition (%) was
obtained by the following formula:
(14) Richecoeur, A. M. E.; Sweeney, J. B. Tetrahedron 2000, 56, 389–
395.
(15) (a) Su, B. N.; Park, E. J.; Mbwambo, Z. H.; Santarsiero, B. D.;
Mesecar, A. D.; Fong, H. H.; Pezzuto, J. M.; Kinghorn, A. D. J. Nat.
Prod. 2002, 65, 1278–1282. (b) Yang, L.; Andersen, R. J. J. Nat. Prod.
2002, 65, 1924–1926. (c) That, Q. T.; Jossang, J.; Jossang, A.; Kim,
P. P.; Jaureguiberry, G. J. Org. Chem. 2007, 72, 7102–7105.
(16) Ohtani, I.; Kusumi, T.; Kashman, Y.; Kakisawa, H. J. Am. Chem.
Soc. 1991, 113, 4092–4096.
(17) Chen, C. Y.; Chen, C. H.; Wong, C. H.; Liu, Y. W.; Lin, Y. S.; Wang,
Y. D.; Hsui, Y. R. J. Nat. Prod. 2007, 70, 103–106.
(18) (a) Kwon, H. C.; Baek, N. I.; Choi, S. U.; Lee, K. R. Chem. Pharm.
Bull. 2000, 48, 614–616. (b) Garcez, F. R.; Garcez, W. S.; Martins,
M.; Matos, M. F. C.; Guterres, Z. R.; Mantovani, M. S.; Misu, C. K.;
Nakashita, S. T. Planta Med. 2005, 71, 923–927.
inhibition (%) ) [(OD(sample)-OD(control))/(OD(normal)
-
OD(control))] × 100
All values were expressed as (SD. The Student’s t-test for unpaired
observations between normal or control and tested samples was carried
out to identify statistical differences; p values less than 0.05 were
considered as significantly different.
(19) Chen, M. J.; Lo, C. Y.; Chin, C. C.; Liu, R. S. J. Org. Chem. 2000,
65, 6362–6367.
(20) (a) Krauss, A. S.; Taylor, W. C. Aust. J. Chem. 1991, 44, 1335–1340.
(b) Carroll, A. R.; Krauss, A. S.; Taylor, W. C. Aust. J. Chem. 1993,
46, 277–292.
(21) Majumder, P. L.; Chatterjee, A.; Sengupta, G. C. Phytochemistry 1972,
11, 811–814.
Acknowledgment. Financial support from the National Natural
Sciences Foundation of China (NNSFC; grant no. 30825044), the
Program for Changjiang Scholars and Innovative Research Team in
University (PCSIRT, grant no. IRT0514), and the National “973”
Program of China (grant nos. 2004CB13518906 and 2006CB504701)
is acknowledged.
(22) (a) The semisystematic nomenclature of 8 and 9 is based on the lignane
parent structure. (b) Moss, G. P. Pure Appl. Chem. 2000, 72, 1493–
Supporting Information Available: Figure S1, main HMBC
correlations for compounds 7, 9-11, and 11a. Figure S2, main NOESY
correlations for compounds 7, 9, 10, 10bR, and 11a. Figure S3, CD
spectra of compounds 7 and (+)-guaiacin (12). Scheme S1, chemical
transformation from 1 to (+)-(5S)-3-dodecyl-5-methylfuran-2(5H)-one
1523.
(23) Yang, X. F.; Wang, M.; Varma, R. S.; Li, C. J. Org. Lett. 2003, 5,
657–660.
(24) (a) House, H. O.; Crumrine, D. S.; Teranishi, A. Y.; Olmstead, H. D.
J. Am. Chem. Soc. 1973, 95, 3310–3324. (b) Heathcock, C. H.; Pirrung,
M. C.; Sohn, J. E. J. Org. Chem. 1979, 44, 4294–4299.
(25) Sato, M.; Sunami, S.; Sugita, Y.; Kaneko, C. Heterocycles 1995, 41,
1435–1444.
(26) Kuo, Y. H.; Lin, S. T. Chem. Pharm. Bull. 1993, 41, 1507–1512.
(27) Fan, P.; Lou, H.; Yu, W.; Ren, D.; Ma, B.; Ji, M. Tetrahedron Lett.
2004, 45, 3163–3166.
(28) The semisystematic nomenclature of 11 is based on the flavane parent
structure.
(29) Hwu, J. R.; Tseng, W. N.; Gnabre, J.; Giza, P.; Huang, R. C. C. J. Med.
Chem. 1998, 41, 2994–3000.
1
(1c) and 1d, and ∆δSR (δS - δR) values obtained from the H NMR
spectra of the MTPA esters of 1a. Scheme S2, chemical transformation
from 9 to 8. Scheme S3, preparation of derivatives of 10 and ∆δSR (δS
1
- δR) values obtained from the H NMR spectra of the MTPA esters
of 10b and 10c. Scheme S4, proposed biogenetic cycloaddition pathway
from (-)-epi-catechin to 11 and viniperferones B and C. IR, MS, 1D
and 2D NMR spectra of 1, 1a-1d, S- and R-MTPA-1a, 2-8, 8a, 8b,
9, 9a, 10, 10a, 10bS, 10bR, 10cS, 10cR, 11, 11a, and 12-17. This
material is available free of charge via the Internet at http://pubs.acs.org.
(30) Suri, K. A.; Sood, R. P.; Suri, O. P.; Atal, C. K.; Singh, G. B. Indian
J. Pharm. Sci. 1981, 43, 226–228.
(31) Hattori, M.; Hada, S.; Kawata, Y.; Tezuka, Y.; Kikuchi, T.; Namba,
T. Chem. Pharm. Bull. 1987, 35, 3315–3322.
(32) Shimomura, H.; Sashida, Y.; Oohara, M. Phytochemistry 1988, 27,
634–636.
References and Notes
(1) (a) Lee, S. K.; Wei, F. N. Guihaia 1984, 4, 93–106. (b) Zhuang, X. Y.
Guihaia 1997, 17, 291–294. (c) Lin, X. Z. Sci. SilVae Sin. 2007, 43,
151–156.
(33) Iida, T.; Nakano, M.; Ito, K. Phytochemistry 1982, 21, 673–675.

2152 Journal of Natural Products, 2009, Vol. 72, No. 12
Cheng et al.
(34) Achenbach, H.; Grob, J.; Dominguez, X. A.; Cano, G.; Star, J. V.;
Del Carmen Brussolo, L.; Munoz, G.; Salgado, F.; Lopez, L.
Phytochemistry 1987, 26, 1159–1166.
(35) Rollinson, S. W.; Amos, R. A.; Katzenellenbogen, J. A. J. Am. Chem.
Soc. 1981, 103, 4114–4125.
(41) (a) Mosmann, T. J. Immunol. Methods 1983, 65, 55–63. (b) Car-
michael, J.; DeGraff, W. G.; Gazdar, A. F.; Minna, J. D.; Mitchell,
J. B. Cancer Res. 1987, 47, 936–942. (c) Mo, S. Y.; Wang, S. J.;
Zhou, G. X.; Yang, Y. C.; Li, Y.; Chen, X. G.; Shi, J. G. J. Nat.
Prod. 2004, 67, 823–828.
(36) Fraga, B. M.; Terrero, D. Phytochemistry 1996, 41, 229–232.
(37) Goldsby, G.; Burke, B. A. Phytochemistry 1987, 26, 1059–1063.
(38) Oksuz, S.; Topcu, G. Phytochemistry 1992, 31, 195–197.
(39) Song, W. X.; Li, S.; Wang, S. J.; Wu, Y.; Zi, J. C.; Gan, M. L.; Zhang,
Y. L.; Liu, M. T.; Lin, S.; Yang, Y. C.; Shi, J. G. J. Nat. Prod. 2008,
71, 922–925.
(42) (a) Xiong, Q.; Hase, K.; Tezuka, Y.; Tani, T.; Namba, T.; Kadota,
S. Planta Med. 1998, 64, 120–125. (b) Xu, F. M.; Morikawa, T.;
Matsuda, H.; Ninomiya, K.; Yoshikawa, M. J. Nat. Prod. 2004, 67,
569–576.
(40) Liu, G. T. Chin. J. New Drugs 2001, 10, 325–327.
NP900504A