Tyrosine bioconjugation through aqueous ene-type reactions: A click-like reaction for tyrosine

Article abstract of DOI:10.1021/ja909062q

(Figure Presented) A new and versatile class of cyclic diazodicarboxamides that reacts efficiently and selectively with phenols and the phenolic side chain of tyrosine through an ene-like reaction is reported. This mild aqueous tyrosine ligation reaction works over a broad pH range and expands the repertoire of aqueous chemistries available for small molecule, peptide, and protein modification. The tyrosine ligation reactions are shown to be compatible with the labeling of native enzymes and antibodies in a buffered aqueous solution. This reaction provides a novel synthetic approach to bispecific antibodies. We believe this reaction will find broad utility in peptide and protein chemistry and in the chemistry of phenol-containing compounds.

Full text of DOI:10.1021/ja909062q

Published on Web 01/12/2010
Tyrosine Bioconjugation through Aqueous Ene-Type Reactions: A Click-Like
Reaction for Tyrosine
Hitoshi Ban, Julia Gavrilyuk, and Carlos F. Barbas, III*
The Skaggs Institute for Chemical Biology and the Departments of Chemistry and Molecular Biology,
The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037
Received October 23, 2009; E-mail: carlos@scripps.edu
Scheme 1. Model Tyrosine Ligation Reaction
Bioconjugation methods rely heavily on chemoselective modi-
fication of native protein functional groups.1 Lysine and cysteine
side chains are the most commonly functionalized amino acids;
however, the high abundance of lysine on protein surfaces makes
site-specific modification challenging. In contrast, cysteines are rare
and most often in disulfide linked pairs in proteins in their natural
environment. Labeling at this amino acid typically requires reduc-
tion of the target disulfide followed by reaction with a reagent like
maleimide. Recently significant attention has been paid to the
bioorthogonal modification of the aromatic amino acid side chains
of tryptophan2 and tyrosine.3 Tyrosine modification in mild,
biocompatible, metal-free conditions has been studied using Man-
nich-type additions to imines.3a-c Inspired by these pioneering
efforts, we sought to capitalize on the reactivity of diazodicarboxy-
late-related molecules to create an efficient aqueous ene-type
reaction as an orthogonal bioconjugation strategy. Here we present
a new and efficient tyrosine ligation reaction (TLR) and demonstrate
the utility of the reaction in the preparation of small molecule,
peptide, enzyme, and antibody conjugates.
Next we studied the chemoselectivity of this reaction with a
defined collection of N-acyl methyl amides of histidine, tryptophan,
serine, cysteine, and lysine. Significantly, only tryptophan6 and
lysine yielded a product detectable by 1H NMR. It is important to
note that the indole of tryptophan reacted equally sluggish with
PTAD when the reaction was performed in neat organic solvent or
in mixed aqueous media suggesting that aqueous conditions
dramatically activate the phenolic group of tyrosine for reaction.
Competition experiments with an equimolar mixture of N-acyl
methyl amides of tyrosine and tryptophan or tyrosine and lysine
resulted in selective modification of tyrosine in 55% and 58%
conversion, respectively, with no detectable modification of other
amino acid amides (see Supporting Information). Similarly, when
an equimolar mixture of all six amino acid amides was treated with
PTAD, only tyrosine modification (39% conversion) was observed
Substituted phenols can react with highly reactive electrophiles
such as diazodicarboxylates in organic solvents in the presence of
activating protic or Lewis acid additives.4 Rapid decomposition of
diazodicarboxylate reagents in aqueous media and/or low reactivity
toward phenols makes them unsuitable for bioconjugation.5 Acyclic
diazodicarboxylate reagents are dramatically activated in ene
reactions by interaction with cationic species such as protons or
metal ions.5 Cyclic diazodicarboxamides like 4-phenyl-3H-1,2,4-
triazole-3,5(4H)-dione (PTAD), however, are not similarly activated,
and this reactivity difference suggested to us that they might present
an opportunity for aqueous chemistry. We conducted a preliminary
survey of the reactivity and stability of diazodicarboxylate and
diazodicarboxamide reagents for the reaction with N-acyl tyrosine
methyl amide 1 in aqueous buffer (data not shown). This study
revealed that the decomposition of acyclic diazodicarboxylates in
aqueous media was faster than the desired reaction with 1, whereas
acyclic diazodicarboxamides were stable but not reactive enough.
Ultimately, PTAD 2 provided the desired reactivity and stability
(Scheme 1). As a model for peptide labeling, we studied N-acyl
tyrosine methylamide 1 modification with PTAD 2 in mixed
organic/aqueous media necessitated by the solubility characteristics
of 1. In sodium phosphate buffer, pH 7/acetonitrile (1:1), peptide
1 reacted rapidly (reaction was complete within 5 min) with 1.1
equiv of PTAD to provide 3 in 65% isolated yield. With the addition
of 3.3 equiv of PTAD, quantitative modification could be obtained
(see Supporting Information). The buffer concentration did not
significantly affect the reaction, and notably, the reaction did not
proceed in acetonitrile alone. To the best of our knowledge this
type of reaction has not been reported to occur in such mild aqueous
media.4
1
by H NMR, indicating that this reagent exhibits a high degree of
chemoselectivity.
Given the inherent reversibility of the reaction between the related
cyclic diazodicarboxamide 4-methyl-1,2,4-triazoline-3,5-dione and
indoles,6 our next concern was the relative stability of the C-N
bond formed in our products. To study this, we used p-cresol as a
model phenol (Scheme 2). Compound 4, the product of the reaction
of p-cresol and PTAD, was subjected to both strongly acidic and
basic conditions for 24 h at room temperature or high temperature
(120 °C) for 1 h. The C-N bond was found to be stable under
these conditions, and starting material was recovered in 89% yield
following acid treatment and quantitatively recovered following base
and heat treatments. These conditions are extremely harsh for a
peptide or protein. This study suggests that the 1,2,4-triazolidine-
3,5-dione linkage is hydrolytically and thermally stable, more robust
than maleimide-type conjugations, which are prone to elimination,
or Mannich-type conjugations where retro-Mannich reactions would
be expected.
We then evaluated this reaction using peptides to assess the
application of this approach in peptide chemistry. The acyclic
tripeptide H-Gly-Gly-Tyr-OH reacted rapidly with PTAD 2 in
phosphate buffer, pH 7/acetonitrile (1:1), to provide product 5 in
85% isolated yield (Figure 1). Similarly, reaction of the small cyclic
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C O M M U N I C A T I O N S
Scheme 2. Stability Evaluation
peptide (Ile3)-pressinoic acid (tocinoic acid) with PTAD provided
product 6 (Figure 1) in 83% isolated yield. No bis-addition products
were observed in any of our studies. These experiments demon-
strated the chemoselectivity of this reaction and its application to
peptide chemistry and suggested that cyclic diazodicarboxamides
like PTAD should possess the reactivity and chemoselectivity
required for complex protein modification.
Figure 2. Linkers and rhodamine dye reagents.
Table 1. Protein Modification Study
Labeling
with
reagent 9,
Labeling
with
reagent 10,
Reagent
concn, mM
b
b
No.
Proteina
%
%
1
2
3
4
5
6
7
8
Chymotrypsinogen A
Chymotrypsinogen A
Chymotrypsinogen A
Myoglobin
Myoglobin
Myoglobin
BSA
BSA
BSA
1
5
10
1
5
10
1
56
72
81
6
6
8
85
96
96
3
3
4
35
54
60
13
13
16
53
65
68
3
Figure 1. PTAD modification of peptides.
To explore the potential of this reaction for protein functional-
ization, we prepared and studied several functionalized PTAD
analogues (Figure 2). Azide containing linkers 7 and 8 were
prepared as stable and synthetically versatile precursors with utility
in click chemistry7 and as intermediates in the synthesis of 9 and
10 (see Supporting Information). Differentially functionalized
PTAD reagents were chosen to study whether the reactivity of these
reagents could be tuned with electronic effects. Reduction of the
azide functionality and reaction with the commercially available
NHS-activated 5- and 6-carboxy-X-rhodamine (ROX) provided the
corresponding amide products. Oxidation to the corresponding
cyclic diazodicarboxamides 9 and 10 (Figure 2) was done with NBS
and pyridine in DMF. To evaluate nonspecific and noncovalent
attachment of highly hydrophobic ROX reagents to protein, the
nonreactive rhodamine alkyne 11 was prepared and used as a
negative control reagent.
Chymotrypsinogen A, bovine serum albumin (BSA), and myo-
globin from equine heart were chosen as model protein systems,
as these proteins have different tyrosine and tryptophan contents
and side chain accessibilities. We studied protein labeling at the
physiological pH 7.4 in phosphate buffer with a minimal amount
of DMF, needed to prepare and deliver the labeling reagent. The
final concentration of DMF in the reaction mixture was 1 to 5%.
PTADs 9 and 10 were studied at concentrations ranging from 1 to
10 mM (Table 1). Assessment of the reaction conversion was done
by UV analysis following dialysis in buffer to remove unbound
dye. Reagent 9 provided up to 81% labeling of chymotrypsinogen
A and up to 96% labeling of BSA (Table 1). Myoglobin was labeled
with reagent 9 at 6-8%. The background nonspecific and nonco-
valent association of rhodamine dye 11 to proteins accounted for
3-4% labeling in this assay. Reagent 10 was expected to be more
reactive and less stable in aqueous media than 9 given the electron-
withdrawing linker; it yielded 60% labeling of chymotrypsinogen
5
9
10
10c
10c
10c
10
11
12
Chymotrypsinogen A
Myoglobin
BSA
3
4
a Protein concentration was kept at 30 µM in 0.1 M phosphate buffer,
pH 7.4. b Conversion was calculated based on UV-vis absorption for
extensively desalted and dialyzed sample. Average conversion of two
independent experiments is shown. c Reagent 11 was used as a negative
control.
A, 68% labeling of BSA, and 16% modification of myoglobin.
Tryptic digest and subsequent ESI-MS analysis of the fragments
of all proteins modified with reagents 9 and 10 confirmed primary
sites for covalent modification of chymotrypsinogen A at Y228 and
BSA at Y355 and Y357. Additional modification sites for BSA
were identified to be Y393 and Y424 in the reaction with 10 mM
reagent 9. In the doubly modified chymotrypsinogen, Y171 was
additionally labeled (Figure 3). Reagent 10 modified myoglobin at
W15 at a very low level, consistent with results of our small
molecule study. Although we detected some modification of
myoglobin with 9, the degree of labeling was too low to identify
the site. Covalent modification of proteins was confirmed using a
gel-based assay, MALDI-TOF, and ESI analysis (Figure 3 and
Supporting Information). Chymotrypsinogen A retained its enzy-
matic activity following labeling consistent with the mild nature
of the reaction (see Supporting Information).
We studied BSA labeling with 9 over a wide pH range (pH 2 to
pH 10) and found significant protein labeling at all pH’s. Up to
54% labeling was observed at pH 2 with labeling ranging from
85% to 98% between pH 7 and 10 (see Supporting Information).
Thus the tyrosine ligation reaction is applicable over a wide pH
range.
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Figure 4. Normalized ELISA for Her/RGD bispecific Ab.
mild aqueous reaction works over a broad pH range and expands
the repertoire of aqueous chemistries available for small molecule,
peptide, and protein modification. We believe this reaction will find
broad utility in protein chemistry and in the chemistry of phenol-
containing compounds.
Figure 3. ESI-MS analysis of purified samples containing (a) unmodified
chymotrypsinogen A and (b) chymotrypsinogen A modified with oxidized
linker 7. (c) Gel stained with coomassie blue (top) and under UV light
(bottom): lane 1, unmodified chymotrypsinogen A; lane 2, chymotrypsi-
nogen A/11; lane 3, chymotrypsinogen A/9; lane 4, unmodified myoglobin;
lane 5, myoglobin/11; lane 6, myoglobin/9; lane 7, unmodified BSA; lane
8, BSA/11; and lane 9, BSA/9.
Acknowledgment. We thank Prof. Phil Baran for useful discus-
sions. This study was supported by the Skaggs Institute for Chemical
Biology.
We envision that this tyrosine ligation reaction might be used
for the bioconjugation of a wide variety of functionalities onto
protein surfaces. To test this hypothesis, an integrin binding cyclic
RGD peptide containing an alkyne, 12, was prepared. Subsequent
Cu(I)-mediated click reaction with intermediate 7 followed by
oxidation with NBS/Py provided the labeling reagent that was then
reacted with the therapeutic antibody herceptin8 (Scheme 3). The
Supporting Information Available: Full experimental procedures
and characterization data are available for all new compounds. This
material is available free of charge via the Internet at http://pubs.acs.org.
References
(1) (a) Aslam, M.; Dent, A. Bioconjugation. Protein Coupling Techniques for
Biomedical Sciences; Grove’s Dictionaries Inc.: New York, NY, 1998. (b)
Sletten, E. M.; Bertozzi, C. R. Angew. Chem., Int. Ed. 2009, 48, 6974–
6998.
(2) (a) Antos, J. M.; Francis, M. B. J. Am. Chem. Soc. 2004, 126, 10256–7. (b)
Antos, J. M.; McFarland, J. M.; Iavarone, A. T.; Francis, M. B. J. Am. Chem.
Soc. 2009, 131, 6301–6308.
Scheme 3. Preparation of Her/RGD Constructa
(3) (a) Joshi, N. S.; Whitaker, L. R.; Francis, M. B. J. Am. Chem. Soc. 2004,
126, 15942–3. (b) McFarland, J. M.; Joshi, N. S.; Francis, M. B. J. Am.
Chem. Soc. 2008, 130, 7639–44. (c) Minakawa, M.; Guo, H. M.; Tanaka,
F. J. Org. Chem. 2008, 73, 8669–72. (d) Kodadek, T.; Duroux-Richard, I.;
Bonnafous, J. C. Trends Pharmacol. Sci. 2005, 26, 210.
(4) (a) Schroete, S. J. Org. Chem. 1969, 34, 4012–14. (b) Mitchell, H.; Leblanc,
Y. J. Org. Chem. 1994, 59, 682–687. (c) Leblanc, Y.; Boudreault, N. J.
Org. Chem. 1995, 60, 4268–4271. (d) Yadav, J. S.; Reddy, B. V. S.; Kumar,
G. M.; Madan, C. Synlett 2001, 1781–1783. (e) Bombek, S.; Lenarsic, R.;
Kocevar, M.; Saint-Jalmes, L.; Desmurs, J. R.; Polanc, S. Chem. Commun.
2002, 1494–1495. (f) Yadav, J. S.; Reddy, B. V. S.; Veerendhar, G.; Rao,
R. S.; Nagaiah, K. Chem. Lett. 2002, 318–319. (g) Kinart, W. J.; Kinart,
C. M. J. Organomet. Chem. 2003, 665, 233–236. (h) Chee, G. L. Synth.
Commun. 2006, 36, 2151–2156. (i) Brandes, S.; Bella, M.; Kjoersgaard, A.;
Jorgensen, K. A. Angew. Chem., Int. Ed. 2006, 45, 1147–1151.
(5) Desimoni, G.; Faita, G.; Righetti, P. P.; Sfulcini, A.; Tsyganov, D.
Tetrahedron 1994, 50, 1821–1832.
(6) Baran, P. S.; Guerrero, C. A.; Corey, E. J. Org. Lett. 2003, 5, 1999–2001.
(7) (a) Rostovtsev, V. V.; Green, L. G.; Fokin, V. V.; Sharpless, K. B. Angew.
Chem., Int. Ed. 2002, 41, 2596–2599. (b) Tornoe, C. W.; Christensen, C.;
Meldal, M. J. Org. Chem. 2002, 67, 3057–3064. (c) Agard, N. J.; Prescher,
J. A.; Bertozzi, C. R. J. Am. Chem. Soc. 2004, 126, 15046–15047. (d) Ning,
X. H.; Guo, J.; Wolfert, M. A.; Boons, G. J. Angew. Chem., Int. Ed. 2008,
47, 2253–2255.
a (a) 7, Cu, CuSO4. (b) (i) NBS, Py, DMF; (ii) herceptin in phosphate
buffer, pH 7.4.
resulting herceptin/RGD conjugate was purified and characterized
by MALDI-TOF MS. ErbB-2 and integrin Rvꢀ3 binding ELISA
(Figure 4) demonstrated that modification of the antibody herceptin
through tyrosine conjugation did not impair its ability to bind to
ErbB-2, while introduction of the cyclic RGD peptide allowed the
antibody conjugate to bind integrin Rvꢀ3, thereby providing a new
chemical route to antibodies with multiple specificities.9
In summary, we have developed a new and versatile class of
cyclic diazodicarboxamides that react selectively with phenols and
the phenol side chain of tyrosine through an ene-like reaction. This
(8) Goldenberg, M. M. Clin. Ther. 1999, 21, 309–18.
(9) Gavrilyuk, J. I.; Wuellner, U.; Salahuddin, S.; Goswami, R. K.; Sinha, S. C.;
Barbas, C. F., III. Bioorg. Med. Chem. Lett. 2009, 19, 3716–3720.
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