Facile methods to recycle nucleosides during solid phase synthesis of oligonucleotides

Article abstract of DOI:10.1016/S0040-4039(00)76822-5

Solid phase syntheses of oligonucleotides, using nucleoside phosphoramidites or methylphosphonamidites require a large excess of nucleoside monomers over the hydroxy functions of the growig oligonucleotide chain bound onto the solid phase. The outlined method allows to recover the excess nucleosides. All the protective groups on the sugar and the nucleobase of the monomers are maintained throughout the recycling process.