Magnetic Resonance in Chemistry p. 123 - 130 (1996)
Update date:2022-08-03
Topics:
Johnson, Christopher D.
Gerig
N-(4-Fluorophenyl)-N-(2,6-difluorophenyl)carbamoyl chloride was synthesized and used to inactivate the serine protease α-chymotrypsin. The derivatized enzyme reactivates sufficiently slowly at pH 5.2 that extended fluorine NMR studies of the system are possible. Such studies show that the 4-fluorophenyl ring can be found in two enzyme environments which are characterized by chemical shifts and relaxation behaviors that are very similar to those obtained in work with related compounds reported previously. The 2,6-difluorophenyl ring is also found in two environments, with rotation of the difluoroaromatic ring being slow in both. Saturation transfer methods were used to estimate the rate of interconversion between environments and the rate of aromatic ring rotation. Experiments with the protein dissolved in 8 M urea, conditions that normally lead to protein denaturation, show that sufficient structure remains about the difluorophenyl ring that rotation is slow under these conditions also, even though the structural features that produce the large fluorine chemical shift effects seen in the native enzyme are absent.
View MoreShandong Bolode Bio-Technology Co., LTD
Contact:+86-0531-58966870
Address:136 Jingyi Road,Huaiyin District,Jinan,Shandong,China
SHIJIAZHUANG HENRYTE CHEMICALS CO,.LTD(expird)
Contact:+86-311-85208698 311-80837698
Address:NO.166, yuhua west road, SHIJIAZHUANG, China
website:http://www.hanwayschem.com
Contact:+86-18502787239(whatsapp)
Address:18-1-802, Green Garden, Jianghan District, Wuhan 430023, China
Shijiazhuang Haitian Amino Acid Co., Ltd.
Contact:+86-311-88908111
Address:Shijiazhuang Hebei province,China
website:http://www.win-winchemical.com
Contact:0086-577-64498589
Address:6F, No. 396 Xingping Road, Longwan Industrial Zone, Wenzhou City, Zhejiang, 325000 P.R.China
Doi:10.1055/s-1996-4193
(1996)Doi:10.1016/S0040-4020(01)00750-5
(2001)Doi:10.1039/CC9960000365
(1996)Doi:10.1021/om9508896
(1996)Doi:10.1021/ja954303i
(1996)Doi:10.1002/jhet.5570330118
(1996)