Magnetic Resonance in Chemistry p. 123 - 130 (1996)
Update date:2022-08-03
Topics:
Johnson, Christopher D.
Gerig
N-(4-Fluorophenyl)-N-(2,6-difluorophenyl)carbamoyl chloride was synthesized and used to inactivate the serine protease α-chymotrypsin. The derivatized enzyme reactivates sufficiently slowly at pH 5.2 that extended fluorine NMR studies of the system are possible. Such studies show that the 4-fluorophenyl ring can be found in two enzyme environments which are characterized by chemical shifts and relaxation behaviors that are very similar to those obtained in work with related compounds reported previously. The 2,6-difluorophenyl ring is also found in two environments, with rotation of the difluoroaromatic ring being slow in both. Saturation transfer methods were used to estimate the rate of interconversion between environments and the rate of aromatic ring rotation. Experiments with the protein dissolved in 8 M urea, conditions that normally lead to protein denaturation, show that sufficient structure remains about the difluorophenyl ring that rotation is slow under these conditions also, even though the structural features that produce the large fluorine chemical shift effects seen in the native enzyme are absent.
View MoreHubei Danao Pharmaceutical Co.,Ltd.
website:http://www.danaopharm.com
Contact:+86-719-5251167
Address:Fandan Road,Danjiangkou,Hubei
Hangzhou Dingyan Chem Co., Ltd
website:http://www.dingyanchem.com
Contact:86-571-87157530-8002
Address:RM.1118,NO.1 Building, Baiyun Tower,Jianggan Area, Hangzhou city, China,310004
Wuxi Morality Chemical Co., Ltd(expird)
Contact:
Address:B/7F, 321th WuYun Rd, Wanda Plaza, Wuxi City, 214174, China
Shanghai HengXun Pharmaceutical Tech. Co., Ltd.
Contact:86-86-52730756
Address:Room 603, No. 240, Tianmuzhong Road, Zhabei, Shanghai, China
Shanghai Kefu Chemical Co.,Ltd.
Contact:+86-21-34616196
Address:Room601-602, Xuhui Business Building, No.168, Yude Road, Shanghai
Doi:10.1055/s-1996-4193
(1996)Doi:10.1016/S0040-4020(01)00750-5
(2001)Doi:10.1039/CC9960000365
(1996)Doi:10.1021/om9508896
(1996)Doi:10.1021/ja954303i
(1996)Doi:10.1002/jhet.5570330118
(1996)