STUDY ON THE BIOSYNTHESIS OF ECDYSONE PART IV: Synthesis of high specific activity -2,22-dideoxyecdysone Tissue distribution of the C-22 hydroxylase in Locusta migratoria

Article abstract of DOI:10.1016/S0040-4020(01)85918-4

We have synthesized a tritiated form of 2,22-dideoxyecdysone <<(3)H2-22,23>-3β,14α,25-trihydroxy-5β-cholest-7-en-6-one> of high specific activity (2.2 TBq/mmol).We have examined the capacity of various endocrine (prothoracic glands, follicle cells) and peripheral (fat body, Malpighian tubules) tissues of Locusta migratoria to use this molecule as a precursor of ecdysone biosynthesis.Efficient conversion of 2,22-dideoxyecdysone to 2-deoxyecdysone and to ecdysone could principally be monitored in the prothoracic glands and follicle cells.