Tetrahedron p. 1397 - 1408 (1988)
Update date:2022-09-26
Topics:
Haag, Thierry
Hetru, Charles
Kappler, Christine
Moustier, Anne Marie
Hoffmann, Jules A.
Luu, Bang
We have synthesized a tritiated form of 2,22-dideoxyecdysone <<(3)H2-22,23>-3β,14α,25-trihydroxy-5β-cholest-7-en-6-one> of high specific activity (2.2 TBq/mmol).We have examined the capacity of various endocrine (prothoracic glands, follicle cells) and peripheral (fat body, Malpighian tubules) tissues of Locusta migratoria to use this molecule as a precursor of ecdysone biosynthesis.Efficient conversion of 2,22-dideoxyecdysone to 2-deoxyecdysone and to ecdysone could principally be monitored in the prothoracic glands and follicle cells.
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