Journal of the American Chemical Society p. 4902 - 4906 (1984)
Update date:2022-09-26
Topics: Steric hindrance Enzyme Kinetics Experimental Design coli Binding site Cofactor
Aberhart, D. John
Russell, David J.
The conversion of α-ketoisovaleric acid (α-KIVA) to ketopantoate by the 5,10-methylenetetrahydrofolate-dependent enzyme ketopantoate hydroxymethyltransferase (KHMT) in E. coli has been shown to proceed in a retention mode at the β-position of α-KIVA. 5,10-methylenetetrahydrofolate formed in vivo by serine hydroxymethyltransferase (SHMT) from stereospecifically deuterated (3S-d1) serine was converted by KHMT into an ca. 3:1 ratio of deuterated ketopantoates with the 4S isomer predominating.The results indicate that KHMT and SHMT have the same overall steric course in E. coli.
View Moreguide(suzhou) fine materials co. ltd
Contact:0512-80972173
Address:21st Building, No.369 Lushan Rd, New District Suzhou China 215129
Shanghai Sungo Technology Chemical Co., Ltd
Contact:0086-21-51385579
Address:Room2010, F/20, Tonghua Plaza, NO 345 Jinxiang Road, Jinqiao Export Processing Zone, Shanghai, 201206 P.R.CHINA
SHANDONG ZHANHUA YONGHAO PHARMACEUTICAL TECH.CO.,LTD
Contact:+86-576-88685096
Address:GENGJU VILLAGE NORTH ONE KILOMETER,ZHANHUA DISTRICT,BINZHOU CITY,SHANDONG PROVINCE,CHINA.
website:http://www.sigmaaldrich.com
Contact:800 558-9160
Address:Industriestrasse 25CH-9471 Buchs SGSwitzerland
Shanghai birch chemical technology co.,ltd
Contact:+86-21-54096810
Address:No.2588,Jungong Road,Shanghai,China
Doi:10.1039/b101242h
(2001)Doi:10.1016/S0040-4020(01)99162-8
(1964)Doi:10.1016/j.bmc.2006.06.020
(2006)Doi:10.1007/BF00899355
(1950)Doi:10.1021/ol0618353
(2006)Doi:10.1016/j.tetasy.2006.07.022
(2006)
