Enhancement of catalytic activity of chemically modified subtilisin Carlsberg in benzene by adjustment of lyophilization conditions

Article abstract of DOI:10.1246/bcsj.73.1277

Alteration of the lyophilization conditions significantly enhanced not only the activity of insoluble native subtilisin Carlsberg but also that of solubilized enzyme in benzene. The catalytic activity (k(cat)/K(m)) for the transesterification reaction of