Chemistry of Natural Compounds, Vol. 40, No. 6, 2004
CHEMICAL STUDY OF Eriobotrya japonica
I. S. Movsumov
UDC 547.972
In continuation of the search for sources of biologically active compounds, we studied flowers of
L.
E. japonica
(Japanese mimosa, Rosaceae), which is cultivated in Azerbaidzhan as a decorative. In contrast with many plants, it flowers in
November—December [1, 2].
Dried flowers (0.5 kg) were collected in December 2002 and extracted successively with hexane, chloroform, and
ethanol. Compound 1 was obtained from the hexane extract; 2, from chloroform.
The ethanol extract was evaporated to dryness and dissolved in water. The aqueous solution was treated with
ethylacetate. Solvent was removed. Fraction crystallization from ethanol produced 3 and 4.
Compound 1 was small needle-like white crystals that were soluble in hexane, chloroform, and ethanol and insoluble
20
in water, mp 138-139°C (ethanol), [α]
-37° ( 0.25, CHCl ); acetate, mp 126-128°C (ethanol). It appeared on TLC
c
3
D
[developer phosphotungstic acid solution (25%) at 100-105°C] as a pink spot and gave a positive reaction with
Burchard—Liebermann and Salkovskii reagents.
Compound 2 was needle-like white crystals that were soluble in chloroform and ethanol and insoluble in hexane and
water. It gave a positive reaction with Burchard—Liebermann and Salkovskii reagents. It appeared on chromatograms as a
20
pink spot, mp 280-282 C (ethanol), [α]
-64° (c 0.94, CHCl ).
3
D
20
Compound 3 was white crystals, mp 232-234°C (ethanol), [α]
-59° (c 0.6, CH OH), R 0.73
f
3
D
(n-C H OH:CH CO H:H O, 4:1:5), soluble in water and ethanol. Hydrolysis by H SO (4%, 3h) cleaved it into quercetin and
4
9
3
2
2
2
4
L-arabinose. Yield of quercetin, 63.5%.
20
Compound 4 was white crystals, mp 219-221°C (ethanol), [α]
-38° (c 0.15, CH OH), R 0.69
f
3
D
(n-C H OH:CH CO H:H O, 4:1:5), soluble in water and ethanol. Acid hydrolysis by H SO (5%) quickly cleaved it into
4
9
3
2
2
2
4
quercetin and D-glucose. Yield of aglycon, 64.3%.
Based on the physicochemical properties and chromatographic behavior (comparison with authenticspecimens), 1 was
identified as β-sitosterol; 2, ursolic acid; 3, quercetin-3-O-α-L-arabinoside; 4, quercetin-3-O-β-D-glucoside [3, 4].
These compounds are widely distributed in various plants.
E. japonica L. from Azerbaidzhan flora was studied for the first time [5].
REFERENCES
1.
R. M. Abbasov, U. M. Agamirov, F. M. Mamedov, and A. M. Sadykhov, Mardakyan Arboretum [in Russian],
Baku (1978), p. 29.
2.
3.
4.
E. V. Vul′f and O. F. Maleeva, World Resources of Useful Plants [in Russian], Nauka, Leningrad (1969), p. 184.
I. S. Movsumov and A. M. Aliev, Khim. Prir. Soedin., 389 (1981).
I. S. Movsumov, Materials of a Scientific Conference Dedicated to the 100th Birthday of A. S. Gasanov, Baku
(2000), p. 88.
5.
Plant Resources of the USSR, Hydroingeaceae—Haloragaceae Families [in Russian], P. D. Sokolov, ed., Nauka,
Moscow (1987), p. 53.
Azerbaidzhan Medical University, Baku. Translated from Khimiya Prirodnykh Soedinenii, No. 6, p. 493, November-
December, 2004. Original article submitted May 26, 2004.
0009-3130/04/4006-0599 ©2004 Springer Science+Business Media, Inc.
599
Movsumov
