Synthesis and activities of a thienyl dihydropyridine series on intracellular calcium in a rat pituitary cell line (GH3/B6)

Article abstract of DOI:10.1016/0223-5234(96)89551-1

The synthesis of a thienyl dihydropyridine series according to the Hantzsch method is described. The influence of these derivatives on intracellular calcium ([Ca²⁺](i)) in GH3 cells was evaluated in vitro using spectrofluorimetry with indol as Ca²⁺ fluorescent probe. We compared their effects on [Ca²⁺](i) and hormone release with those of nifedipine. The most active tested compounds on [Ca²⁺](i) were those methylated on the 3-position of the thienyl ring (activity was about 75% of nifedipine). Interestingly, the most efficient compounds on [Ca²⁺](i) were also the most efficient on hormone release.