An improved synthesis of pennogenin

Article abstract of DOI:10.1016/j.tet.2018.11.009

An improved synthesis of pennogenin, a bioactive component of Chinese herb “Chonglou” (Paris), is described. A ring-switching process opened the ring E of diosgenin and allowed the use of a hydroxyl-directed diboration/oxidation to introduce C17α-OH, hence eliminating the use of OsO₄. This strategy might be rendered to synthesize similar steroids with C17α-OH.

Full text of DOI:10.1016/j.tet.2018.11.009

Accepted Manuscript
An improved synthesis of pennogenin
Ran Gao, Yong Shi, Wei-Sheng Tian
PII:
S0040-4020(18)31336-X
https://doi.org/10.1016/j.tet.2018.11.009
TET 29920
DOI:
Reference:
To appear in: Tetrahedron
Received Date: 2 October 2018
Revised Date: 3 November 2018
Accepted Date: 5 November 2018
Please cite this article as: Gao R, Shi Y, Tian W-S, An improved synthesis of pennogenin, Tetrahedron
(2018), doi: https://doi.org/10.1016/j.tet.2018.11.009.
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An Improved Synthesis of Pennogenin
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Ran Gao, Yong Shi* and Wei-Sheng Tian*
1.

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An Improved Synthesis of Pennogenin
Ran Gao, Yong Shi* and Wei-Sheng Tian*
CAS Key Laboratory of Synthetic Chemistry of Natural Substances, Shanghai Institute of Organic Chemistry, Chinese
Academy of Sciences, 345 Lingling Road, Shanghai 200032, China
Email: chemyshi@hotmail.com (Y. Shi); wstian@sioc.ac.cn (W. -S. Tian)
Keywords:
Pennogenin; chonglou; diosgenin; diboration/oxidation; C17α-OH steroid
ABSTRACT:
An improved synthesis of pennogenin, a bioactive component of Chinese herb “Chonglou”
(Paris), is described. A ring-switching process opened the ring E of diosgenin and allowed the
use of a hydroxyl-directed diboration/oxidation to introduce C17α-OH, hence eliminating the
use of OsO4. This strategy might be rendered to synthesize similar steroids with C17α-OH.
1

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1. Introduction
Recently, steroidal natural products with a C17α-OH, due to their interesting structures and diverse
bioactivities, have drawn much attention from organic chemists.1-10 The presence of the C17α-OH was
reported to be crucial to their bioactivities.11,12 Our group has long been attracted by these compounds and
completed the syntheses of cephalostatin 1 (1)3 and the aglycone of OSW-1 (2)13 (Figure 1). Meanwhile,
we also explored the synthesis of pennogenin (3), a less complex but potentially more useful natural steroid
with C17α-OH.
Figure 1. Selected Examples of Natural Steroids with C17α-OH
Pennogenin (3) and its glycosides exist widely in plants used in traditional Chinese herbal medicine,
and possess a variety of biological activities, such as antibiotic and antitumor activities, and are widely used
as haemostatic agents. For example, pennogenin glycoside 4 was isolated from Chinese herb Rhizoma
Paridis (Chonglou), and exhibits potent antitumor activity with the IC50 values ranging from 0.5 to 5.1
ꢀg/mL against human promyelocytic leukemia HL-60 cells.14 Natural 3 and its glycosides are not easy to
harvest from natural resources, because they are mixed with many other highly polar steroidal saponins and
3 itself could not survive the acidic hydrolysis that isolation of steroidal sapogenins routinely requires. As
the demand of pennogenin and the related drugs was increasing and the plant resource decreasing, further
development of some Chinese traditional medicines encountered the supply problem of 3 and its glycosides.
Chemical synthesis of 3 might be a practical solution to this problem.
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Scheme 1. Our Previous Synthesis of Pennogenin and Brief Plan for an Improved Synthesis
In 2004, we completed our first synthesis of pennogenin from diosgenin (5), a cheap and readily
available steroidal sapogenin widely used in steroidal industry (Scheme 1).15 Compared with diosgenin,
pennogenin has an extra C17α-OH. To introduce this C17α-OH, one ideal method might be through direct
C17-H oxidation, which, although reported occasionally16-18, was not yet synthetically usable. We thus
adopted a less direct approach, namely, to open spirokeal rings EF and introduce the C17α-OH and re-close
the rings. As outlined briefly in Scheme 1, this synthesis was accomplished in 13 steps with an overall yield
of 10%, with serveral problems remained. The C16-H oxidation with potassium peroxymonosulfate (oxone)
took days to complete, the C5-C6 double bonds needed protection, the dihydroxylation of C16-C17 double
bond required stoichiometric amount of OsO4, and the environmentally unfriendly ethanedithiol and
hydrogen sulfide were used. Aiming to minimize/exclude the use of OsO4 and other unfavorable reagents,
our second synthesis started a decade later based on intermediate 9, which was prepared via a new ring-
opening process we recently developed 19-21. Herein, we would like to report this improved synthesis.
2. Results and discussion
As shown in Scheme 2, the synthesis began with opening the ring E of diosgenin (5) to make C17
modifiable. The C3-OH of diosgenin was acetylated, the ring F was opened reductively, and the resultant
C26-OH was oxidized with Jones reagent to provide furostan-26-acid 10 in 83% yield. Using
trifluoroacetic anhydride as an activation agent and lithium iodide as a nucleophile in DCM/MeCN
provided lactone 9, which, upon treating with lithium bromide and lithium carbonate in DMF at 120 °C,
underwent bromination-elimination to provide 11 in 78% yield from acid 10.
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22
H
22
4. TFAA, LiI, Bu4NI, DCM
diosgenin (5)
MeCN, 0 °C to rt, 8 h, 89%
or TFAA, NaI, DCM/MeCN
O
O
O
O
H
H
17
26
16
16
OH
H
5. LiBr, Li2CO3, DMF
120 °C, 6 h, 78% for 2 steps
H
H
1. Ac2O, py, CH2Cl2, 99%
2. BF3•OEt2, Et3SiH, 96%
2. Jones oxidation, 87%
AcO
5
H
H
10
ring-switching process
AcO
11
6
OsO4 (20 mol%), NMO
cirtric acid, t-BuOH/H2O
40 °C, 48 h, 36%
OsO4 (20 mol%), NMO
cirtric acid, t-BuOH/H2O
40 °C, 48 h, 72%
O
O
O
OH
O
H
O
OH
O
H
H
OH
H
OH
H
H
H
H
H
H
AcO
AcO
13
14
H
H
AcO
12
Scheme 2. Open the Ring E of Diosgenin and Oxidize with OsO4.
With alkene 11 in hand, we first tried to run dihydroxylation of C16-C17 double bond with less OsO4.
Previously, we have performed the dihydroxylation of 13 with a catalytic amount of OsO4 in the presence
citric acid22,23 at 40 °C to deliver diol 14 in 72% yield, which allowed us to synthesize the aglycon of
aspafilioside E, 5α,6-dihydropennogein.20 However, on 11 the selectivity issue (C16-C17 vesus C5-C6)
emerged. The same procedure provided the desired 12 in 36% yield, along with comparable amount of
over-oxidation product. It should be noted that oxidation with stoichiometric amount of OsO4 at low
temperature also did not show the same selectivity as in our fisrt synthesis. Such selectivity was not good
enough to guarantee an efficient synthesis of pennogenin.
Further optimization was focused on excluding the use of toxic OsO4 and improving the selectivity of
oxidation. One option was to use the C22-OH as a directing group. Since opening the lactone ring of 11
through methanolysis was reversible and might cause epimerization at C25,19,21 we decided to reduce it to a
diol. Lactone 11 was therefore treated with LiAlH4, and the resulting C3-OH and C26-OH were selectively
protected as TBDPS ethers, leaving the C22-OH exposed for further use (Scheme 3).
Selective oxidation of double bonds directed by an adjacent hydroxyl group have been extensively
investigated. The epoxidation of 15 with VO(acac)2/t-BuOOH system could deliver the desired 16α,17α-
epoxide in 66% yield (structure not showed). Since the 16α,17α-epoxides undergo Wagner-Meerwein
rearrangement easily under most acidic medium systems that could promote an intramolecular endo-type or
intermolecular O-epoxide-opening process, this compound was useless for our synthesis.
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Scheme 3. Completion of the Synthesis of Pennogenin
We then looked for other OH-directing reactions. Recently, Morken and co-workers developed an
alkoxide-catalyzed directed diboration of alkenyl alcohols, which could be used as a dihydroxylation
method.24-26 The diboration reportedly benefited from substrate directiong effects and we therefore assumed
it could react with the C16-C17 double bond of 15 in the presence of the C5-C6 double bond. Indeed,
homoallylic alcohol 15 was heated with B2(pin)2 (16) and Cs2CO3 in MeOH/THF for 6 h, and the resulting
diboration product 17 underwent an oxidative workup (NaOH, H2O2) to give triol 18 in 74% yield on gram
scale.
Having installed the C17α-OH, we then moved to rebuild the spiroketal rings, and the method in our
first synthesis was adopted. Swern oxidation of triol 18 (large access of reagents) gave diketone 19 in
moderate yield. Selective reduction of the C16-ketone with LiAlH4 at -78 °C delivered hemiketal 20 in
excellent yield (85%, 92% based on recovery of starting material). Treating 20 with aqueous HF solution
removed the TBDPS ethers that protected the C3-OH and C26-OH, and promoted the formation of
spiroketal, providing pennogenin (3) in 81% yield. This route delivered pennogenin from diosgenin in ten
steps with an overall yield of 15%. The NMR data of synthetic 3 matched those reported.
3. Conclusion
In summary, we developed an improved synthesis of pennogenin using a ring-switching process to
open the ring E and a hydroxyl-directed diboration/oxidation process to introduce 16α,17α-diol selectively.
Although using nine steps to install a C17α-OH appears far from ideal, this synthesis provides an example
of hydroxyl-directed stereoselective diboration/oxidation in the context of complex natural product
synthesis, which excludes the use of highly toxic OsO4 and might be applicable to the synthesis of similar
steroids with C17α-OH, such as OSW-1.
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4. Experimental Section
General Information: All reactions sensitive to air or moisture were performed in flame-dried round
bottom flasks with rubber septum under a positive pressure of argon or nitrogen atmosphere, unless
otherwise noted. Air and moisture-sensitive liquids and solutions were transferred via syringe and stainless
steel cannula. The solvents and reagents, when needed, was purified according to the standard procedures
described in Purification of Laboratory Chemicals (2009). Yields refer to chromatographically and
spectroscopically (1H NMR) homogeneous materials, unless otherwise stated. Reactions were monitored by
thin-layer chromatography (TLC) carried out on silica gel plates using UV light as visualizing agent and an
ethanolic solution of phosphomolybic acid, and heat as developing agents. NMR spectra were recorded on
Bruker DRX-400 instrument and calibrated using residual undeuterated solvent as an internal reference [1H
NMR: CHCl3 (7.26); 13C NMR: CDCl3 (77.16)]. The following abbreviations were used to explain the
multiplicities: s = singlet, d = doublet, t = triplet, q = quartet, br = broad.
4.1.1 Lactone 11
Under a positive pressure of nitrogen, to a solution of 10 (3.32 g, 7.0 mmol) and sodium iodide (3.15 g,
21 mmol) in CH2Cl2/MeCN (40 mL/10 mL) was added (CF3CO)2O (TFAA, 2.0 mL, 13 mmol) at 0 °C. The
resulting mixture was vigorously stirred at ambient temperature for 12 h, quenched with saturated aqueous
NaHCO3 and saturated aqueous Na2SO3, diluted with water, and extracted with CH2Cl2 (80 + 40 mL). The
combined organic layers were washed with brine, dried over Na2SO4, filtered, concentrated under reduced
pressure, and used in the next step without purification.
Under nitrogen, to a solution of the crude in DMF (40 mL) were added lithium carbonate (1.66 g, 20.6
mmol) and lithium bromide (1.47 g, 13.6 mmol). The mixture was stirred at 120 °C for 6 h and
concentrated under reduced pressure to remove the solvent. The residue was diluted with water and
extracted with EtOAc (120 + 80 mL); the combined oganic layers were washed with brine, dried over
sodium sulfate, filtered, concentrated in vacuo, and purified through silica gel chromatography (PE/EA: 7/1)
to give 11 (2.48 g, 78%) as a white solid. 1H NMR (400 MHz, CDCl3) δ 5.50 (s, 1H), 5.39 (d, J = 4.6 Hz,
1H), 4.65 – 4.55 (m, 1H), 4.42 – 4.33 (m, 1H), 2.66 – 2.54 (m, 1H), 2.53 – 2.43 (m, 1H), 2.03 (s, 3H), 1.21
13
(d, J = 6.7 Hz, 3H), 1.07 (d, J = 7.2 Hz, 3H), 1.05 (s, 3H), 0.82 (s, 3H); C NMR (100 MHz, CDCl3) δ
176.55, 170.69, 155.80, 140.04, 124.31, 122.58, 80.61, 74.03, 57.45, 50.69, 47.14, 38.26, 37.05, 36.94,
36.47, 34.83, 33.21, 31.66, 31.41, 30.60, 27.88, 25.75, 22.93, 21.59, 20.82, 19.38, 16.60, 16.47, 16.44;
HRMS-MALDI (m/z): [M+Na]+ calcd for C29H42O4: 477.2975, found: 477.2977.
4.1.2 Diol 12
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To a solution of cirtric acid (1.26 g, 6.5 mmol) and 11 (910 mg, 2.0 mmol) in t-BuOH/water (20
mL/10 mL) were added OsO4 (4.0 mL, 0.10 M in t-BuOH, 4.0 mmol) and NMO (1.25 mL, 4.0 M solution,
6.0 mmol). The mixture was stirred at 40 °C for 48 h, quenched with saturated aqueous Na2SO3, and
extracted with CHCl3 (30 + 15 mL). The combined organic layers were washed with brine, dried over
NaSO4, filtered, and concentrated under reduced pressure. The crude product was purified through flash
column chromatography on silica gel (PE/EA: 2/1 to 1/1) to give the starting material 11 (112 mg) and 12
(311 mg, 36% based on recovery of 11) as white solid. 1H NMR (400 MHz, CDCl3) δ 5.37 (d, J = 4.2 Hz,
1H), 4.64 – 4.57 (m, 2H), 4.32 (d, J = 7.2 Hz, 1H), 2.87 (brs, 1H), 2.68 – 2.56 (m, 1H), 2.14 (dd, J = 12.8,
6.3 Hz, 1H), 2.03 (s, 3H), 1.21 (d, J = 6.8 Hz, 3H), 1.03 (dd, J = 10.9, 3.9 Hz, 3H), 0.82 (s, 3H). 13C NMR
(100 MHz, CDCl3) δ 176.74, 170.69, 139.76, 122.43, 82.86, 79.95, 76.11, 74.01, 49.46, 48.89, 48.81, 43.54,
38.19, 36.98, 36.62, 35.96, 33.28, 32.94, 31.91, 31.78, 27.84, 25.92, 23.91, 21.58, 20.51, 19.40, 16.34,
14.62, 10.49. ESI-MS (m/z): 511.2, [M+Na] +.
4.1.3 Di-TBDPS-protected triol 15
To a solution of lactone 11 (1.20 g, 2.6 mmol) in dry THF (50 mL) was added LiAlH4 (400 mg, 10.5
mmol) at 0 °C under argon. The mixture was stirred for several hours, and quenched by adding water (0.40
mL), 10% aqueous NaOH solution (0.80 mL), and water (1.2 mL) sequentially. The mixture was filtered
and washed with dry THF. The filtrate was concentrated to dryness and dissolved in dry DCM (40 mL). To
the solution were added imidazole (760 mg, 11.1 mmol) and TBDPSCl (1.8 mL, 6.9 mmol). The mixture
was stirred at ambient temperature for several hours, quenched with a saturated aqueous solution of
NaHCO3, and extracted with DCM (30 mL × 3). The combined organic layers were washed with brine,
dried over Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude product by
flash column chromatography on silica gel (PE/EA: 120/1 to 50/1) provided 15 (1.78 g, 75%) as a white
foam. [α]D −37.0 (c 0.6, CHCl3); IR (KBr): 3070, 2930, 2856, 1471, 1461, 1427, 1111, 701 cm-1; H
NMR (400 MHz, CDCl3) δ 7.71 – 7.65 (m, 8H), 7.44 – 7.34 (m, 12H), 5.47 (s, 1H), 5.17 – 5.12 (m, 1H),
3.55 (m, J = 9.8, 6.9 Hz, 3H), 3.47 (dd, J = 9.8, 6.4 Hz, 1H), 1.07 (s, 9H), 1.06 (s, 9H), 1.03 (s, 3H), 0.95
22
1
13
(dd, J = 6.8, 4.8 Hz, 6H), 0.79 (s, 3H); C NMR (100 MHz, CDCl3) δ 158.6, 141.7, 135.9, 135.9, 135.8,
134.9, 134.9, 134.2, 134.2, 129.6, 129.6, 127.7, 127.6, 127.6, 123.2, 121.0, 110.1, 74.0, 73.3, 69.2, 57.6,
50.75, 47.3, 42.6, 40.2, 37.3, 36.9, 36.1, 34.8, 32.0, 31.7, 31.4, 31.1, 30.7, 29.3, 27.15, 27.0, 20.8, 19.5,
19.5, 19.3, 18.1, 17.0, 16.3; HRMS-ESI (m/z): [M-H]- calcd for C59H80O3Si2: 891.5562, found: 891.5553.
4.1.4 Triol 18
To a suspension of 15 (1.70 g, 1.9 mmol) and Cs2CO3 (360 mg, 1.1 mmol) were added
bis(pinacolato)diboron (16, 2.18 g, 8.6 mmol) and MeOH (1.80 mL) at ambient temperature under argon.
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The vessel was sealed and placed in an oil bath at 80 °C for 6 h. The reaction was allow to cool to 0 °C. To
the mixture was added an aqueous solution of NaOH (1.9 g, 47 mmol), and added dropwise 30% aqueous
soltuion of H2O2 (2.0 mL). After the diboration product disappeared on TLC, the reaction was cautiously
quenched with saturated aquous Na2S2O3 solution, diluted with water, and extracted with ethyl acetate (40
mL × 3). The combined organic layers were washed with brine, dried over Na2SO4, filtered, and
concentrated under reduced pressure. Purification of the crude by flash column chromatography on silica
gel (PE/EA: 12/1 to 10/1) provided triol 18 (1.31 g, 74%) as a white foam. [α]D29 −22.4 (c 0.6, CHCl3); IR
1
(KBr): 3392, 2931, 2897, 2857, 1472, 1462, 1387, 1111, 702 cm-1; H NMR (400 MHz, CDCl3) δ 7.70 –
7.62 (m, 8H), 7.45 – 7.32 (m, 12H), 5.12 (s, 1H), 4.25 (d, J = 7.3 Hz, 1H), 3.70 (t, J = 7.2 Hz, 1H), 3.58 –
3.43 (m, 3H), 3.23 (s, 1H), 1.05 (d, J = 2.0 Hz, 18H), 0.97 (s, 3H), 0.93 (d, J = 6.8 Hz, 3H), 0.88 (d, J = 6.6
Hz, 3H), 0.77 (s, 3H); 13C NMR (100 MHz, CDCl3) δ 141.3, 135.9, 135.9, 135.7, 135.7, 134.9, 134.9,
133.9, 129.8, 129.6, 129.6, 127.8, 127.6, 127.6, 121.1, 82.8, 76.2, 73.8, 73.3, 69.0, 49.4, 48.7, 44.6, 42.6,
37.2, 36.5, 35.5, 34.0, 33.3, 32.7, 31.9, 31.9, 31.8, 28.2, 27.1, 27.0, 20.6, 19.5, 19.4, 19.3, 16.9, 14.1, 13.2.
HRMS-ESI (m/z): [M+H]+ calcd for C59H82O5Si2: 926.5774, found: 927.5746.
4.1.5 Swern oxidation led to diketone 19
To a solution of oxalyl chloride (0.087 mL, 1.0 mmol) in dry DCM (4.0 mL) was slowly added dry
DMSO (0.144 mL, 2.0 mmol) at -78 °C under argon. After the evolution of gas ceased (ca. 30 min), a
solution of alcohol 18 (92.0 mg, 0.10 mmol) in dry DCM (4 mL) was slowly added to the resulting cold
solution. After 35 min, Et3N (0.21 mL, 1.5 mmol) was added and the reaction was left to reach ambient
temperature. After 2 h, the reaction was quenched by addition of water. The organic layer was separated
and the aqueous phase was extracted with DCM (15 mL × 3). The combined organic layers were washed
with brine, dried over Na2SO4, filtered, and concentrated under reduced pressure. Purification of the crude
by flash column chromatography on silica gel (PE/EA: 40/1 to 10/1) provided ketone 19 (52 mg, 56%) as a
white foam. [α]D29 −75.9 (c 0.5, CHCl3); IR (KBr): 3374, 3070, 2932, 2857, 1742, 1696, 1471, 1461, 1427,
1
1379, 1110, 702 cm-1; H NMR (400 MHz, CDCl3) δ 7.71 – 7.62 (m, 8H), 7.39 (m, J = 16.9, 9.6, 4.8 Hz,
12H), 5.55 (s, 1H), 5.11 (d, J = 4.7 Hz, 1H), 3.61 – 3.43 (m, 4H), 1.09 (d, J = 7.2 Hz, 3H), 1.06 (s, 9H),
13
1.05 (s, 9H), 1.01 (s, 3H), 0.94 (d, J = 6.6 Hz, 3H), 0.74 (s, 3H). C NMR (100 MHz, CDCl3) δ 219.5,
217.5, 141.5, 135.9, 135.8, 134.9, 134.9, 134.1, 134.0, 129.6, 129.6, 129.6, 127.7, 127.6, 127.6, 120.6, 85.9,
73.2, 68.7, 49.3, 45.3, 45.2, 43.0, 42.5, 41.2, 37.0, 36.7, 35.5, 35.3, 31.9, 31.2, 30.2, 27.2, 27.0, 26.7, 20.2,
19.6, 19.5, 19.3, 17.0, 13.9, 11.8. HRMS-ESI (m/z): [M+H]+ calcd for C59H78O5Si2: 923.5461, found:
923.5430.
4.1.6 Hemiketal 20
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To a solution of diketone 19 (26 mg, 0.028 mmol) in dry THF (4 mL) was added LiAlH4 (22 mg, 0.58
mmol) at -78 °C under argon. The reaction was stirred for 2 h, quenched by addition of EtOAc, and diluted
with EtOAc (30 mL). The organic layer was washed with brine, dried over Na2SO4, filtered, and
concentrated under reduced pressure. Purification of the crude by flash column chromatography on silica
gel (PE/EA: 10/1 to 8/1) provided 20 (22 mg, 85%, 92% brsm) as a white foam and the starting material 19
(2.0 mg). [α]D29 −40.3 (c 0.7, CHCl3); IR (KBr): 2960, 2924, 2854, 1749, 1734, 1457, 1261, 1020, 800 cm-
1; 1H NMR (400 MHz, CDCl3) δ 7.72 – 7.61 (m, 8H), 7.40 (m, J = 14.7, 7.6 Hz, 12H), 5.11 (s, 1H), 4.14 (m,
J = 14.5, 7.1 Hz, 1H), 3.60 – 3.41 (m, 3H), 1.06 (s, 9H), 1.05 (s, 9H), 1.01 (s, 3H), 0.93 (d, J = 6.7 Hz, 3H),
0.80 (s, 3H); 13C NMR (100 MHz, CDCl3) δ 141.4, 135.9, 135.9, 135.8, 134.9, 134.9, 134.1, 129.7, 129.6,
129.6, 127.7, 127.6, 127.6, 120.9, 111.57, 90.8, 73.3, 68.8, 52.8, 49.7, 44.2, 42.7, 42.6, 37.3, 36.7, 36.0,
35.2, 32.1, 32.0, 31.8, 31.6, 31.3, 27.2, 27.0, 26.8, 20.7, 19.6, 19.4, 19.3, 17.1, 17.0, 8.6; HRMS-ESI (m/z):
[M+H]+ calcd for C59H80O5Si2: 925.5617, found: 925.5601.
4.1.7 Pennogenin (3)
To a solution of hemiketal 20 (18 mg, 0.019 mmol) in THF (3.0 mL) was added 30% aqueous HF
solution (0.50 mL) at ambient temperature. After TLC showed the completion of the reaction, the reaction
was quenched with a saturated aqueous NaHCO3 solution. The mixture was extracted with EtOAc (15 mL
× 3). The combined organic layers were washed with brine, dried over Na2SO4, filtered, and concentrated
under reduced pressure. Purification of the crude by flash column chromatography on silica gel (PE/EA:
8/1 to 3/1) provided pennogenin 3 (6.0 mg, 81%) as a white solid. mp 236 °C; [α]D20 −111 (c 0.234, CHCl3);
IR (KBr): 3533, 3489, 1059, 977 cm-1; 1H NMR (400 MHz, CDCl3) δ 5.34 (d, J = 5.0 Hz, 1H), 3.97 (t, J =
7.8 Hz, 1H), 3.53 – 3.46 (m, 2H), 3.37 (t, J = 10.8 Hz, 1H), 1.02 (s, 3H), 0.90 (d, J = 7.2 Hz, 3H), 0.81 (s,
13
3H), 0.79 (d, J = 6.3 Hz, 3H); C NMR (100 MHz, CDCl3) δ 140.8, 121.3, 110.1, 90.9, 90.1, 71.7, 66.8,
52.8, 49.6, 44.6, 43.7, 42.2, 37.2, 36.6, 32.0, 31.6, 31.6, 31.5, 31.2, 30.7, 30.0, 28.1, 20.6, 19.4, 17.1, 17.0,
8.1; HRMS-MALDI (m/z): [M+Na]+ calcd for C27H42O4: 453.2976, found: 453.2975.
Acknowledgments
We thank the National Natural Science Foundation of China (21572248, 20372078) for financial
support.
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