Isolation and Structure of Bryostatins 12 and 13

Article abstract of DOI:10.1021/jo00389a037

Bryostatins 12 (4, 3.7 mg) and 13 (5, 0.7 mg) were isolated from ca. 1000 kg of the marine bryozoan Bugula neritina (Linnaeus) found in the Eastern Pacific Ocean (California).The new bryostatins led to strong cell growth inhibitory (PS cell line ED50 0.014 and 0.0054 μg/mL) and antineoplastic activity (for bryostatin 12, a 47-68percent increase in life extension at 30-50 μg/kg) against the marine P388 lymphocytic leukemia (PS system).In addition to the previously known constituents (bryostatin 1-3) of Bugula neritina from this ocean area the presence of bryostatins 8(13.2 mg) and 9(16.4 mg) was also established.A detailed series of 1H-1H COSY, 2D-J-resolved, 1H-13C 2D-shift correlated, and 1H-<1H> NOE difference 1H NMR experiments combined with 13C NMR and SP-SIMS studies were employed to elucidate the structures of bryostatins 12 and 13.