Carbohydrate Research p. 151 - 158 (1983)
Update date:2022-08-16
Topics:
Liu, Te-ning E.
Wolf, Beverly
Geigert, John
Neidleman, Saul L.
Chin, Janice D.
Hirano, David S.
The production of solid D-arabino-hexos-2-ulose (D-glucosone) from D-glucose by use of an enzyme, pyranose-2-oxidase (EC 1.1.3.10), is described.The enzyme is extracted from the mycelia of Polyporus obtusus, partially purified, and then immobilized on activated CH-Sepharose 4B.The enzymic conversion of D-glucose into D-glucosone is simple and convenient, and provides a product free from residual D-glucose.Lyophilization of the filtered reaction-solution yields the product, solid D-glucosone.Assay methods have been developed for monitoring the enzymic reaction and evaluating the purity of the final product.
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(1978)
