Bioscience, Biotechnology and Biochemistry p. 1051 - 1055 (1999)
Update date:2022-08-17
Topics:
Yamamoto, Hiroaki
Kawada, Naoki
Matsuyama, Akinobu
Kobayashi, Yoshinori
A gene encoding a stereo-specific secondary alcohol dehydrogenase (CpSADH) that catalyzed the oxidation of (S)-1,3-BDO to 4-hydroxy-2-butanone was cloned from Candida parapsilosis. This CpSADH-gene consisted of 1,009 nucleotides coding for a protein with M, 35,964. A recombinant Escherichia coli JM109 strain harboring the expression plasmid, pKK-CPA1, produced (R)-1,3-BDO (93.5% ee, 94.7% yield) from the racemate without any additive to regenerate NAD+ from NADH.
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Doi:10.1016/j.poly.2019.05.012
(2019)Doi:10.1039/P19800000119
(1980)Doi:10.1016/S0040-4020(03)01061-5
(2003)Doi:10.1055/s-2006-942447
(2006)Doi:10.1021/ja00331a076
(1984)Doi:10.1021/ja01395a029
(1928)
