Chemistry and biodiversity p. 1579 - 1590 (2010)
Update date:2022-08-29
Topics:
Gogami, Yoshitaka
Kobayashi, Ai
Ikeuchi, Toshihiko
Oikawa, Tadao
We succeeded in constructing the Glu219Ala/Asp225Ala (i.e., E219A/D225A) serine racemase (SerR) by site-directed mutagenesis, and the effects of Mg 2+ on the catalytic efficiency and the structure were compared between the E219A/D225A-SerR and the wild-type protein. This is the first example of a serine racemase whose amino acid residues in the Mg 2+-binding site were replaced with other amino acids by site-directed mutagenesis. Neither the serine racemase nor the dehydratase activities of the E219A/D225A-SerR were affected by the addition of Mg2+, and Glu219 and Asp225 of the SerR are the essential amino acid residues for Mg2+ to affect both kinds of enzyme activities. Therefore, Glu219 and Asp225 mediate the effects of Mg2+ on the activity and are important for the SerR to form the Mg2+-binding site. Judging from the difference of the Keq values between the E219A/D225A-SerR and the SerR, Mg2+ might affect the equilibrium states in the racemase reaction. The fluorescence quenching analysis of the E219A/D225A-SerR showed that Mg2+ bound to Glu219 and Asp225 of the SerR probably causes a conformational change in the ternary structure of the SerR.
View MoreHuangshi Meifeng Chemical Co.,ltd.
Contact:+86-714-6516706
Address:1941-4-3#,Hubin Avenue,Huangshi,Hubei,China
SuZhou Ascepion Pharmaceuticals, Inc.
Contact:0512-86881668
Address:Building C,68Xingqing Road,Suzhou,China.
Contact:86-25-58619180
Address:Nanjing High-Tech Zone 10 Xinghuo Road Pukou District Nanjing, Jiangsu 210061 The People's Republic of China
Shandong General Materials Co.,Ltd(Shandong Aoertong Chemical Co., Ltd)
Contact:86-531-88072280
Address:No. 1825 Hualong Road, Licheng District, Jinan, Shandong, China
Shenzhen ZheYi Chemicals Co.,LTD(Shanghai Branch)
Contact:+86-21-54159691
Address:Room1002,Building No.2, Lane 98 Bixiu Road,Minhang District, Shanghai,China 201100
Doi:10.1016/j.tet.2005.11.059
(2006)Doi:10.1016/j.tetlet.2017.05.006
(2017)Doi:10.1016/j.ejmech.2013.10.054
(2013)Doi:10.1021/ja00054a002
(1993)Doi:10.1021/j100289a041
(1987)Doi:10.1016/S0040-4039(03)01800-8
(2003)