Galactosyl transfer onto p-nitrophenyl beta-D-glucoside using beta-D-galactosidase from Bacillus circulans.

Article abstract of DOI:10.1271/bbb.61.1118

beta-D-Gal-(1-->4)-beta-D-Glc-OC6H4NO2-p and its isomers (beta-D-Gal-(1-->3)-beta-D-Glc-OC6H4NO2-p and beta-D-Gal-(1-->6)-beta-D-Glc-OC6H4NO2-p) were synthesized from lactose and beta-D-Glc-OC6H4NO2-p, using transglycosylation by the beta-D-galactosidase from Bacillus circulans. This reaction was efficient enough for us to do a one-pot preparation of galactosyl-glucoside from lactose. The order of the production of the transfer products was (1-->4) > > (1-->3) > (1-->6) in the initial stage of the reaction, and the same relationship was observed for the hydrolytic rate toward the three galactosyl-glucosides. The production of (1-->4)- and (1-->3)-linkages greatly decreased during the subsequent reaction and much more of the (1-->6)- than of the (1-->4)- and (1-->3)-transfer products was found in the later stage of the reaction.

Full text of DOI:10.1271/bbb.61.1118

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Galactosyl Transfer onto p-Nitrophenyl β-d-Glucoside
Using β-d-Galactosidase from Bacillus civculans
Takeomi Murataa, Satoru Akimotoa, Miki Horimotoa & Taichi Usuia
a Faculty of Agriculture, Department of Applied Biological Chemistry, Shizuoka
University, 836 Ohya, Shizuoka 422, Japan
Published online: 12 Jun 2014.
To cite this article: Takeomi Murata, Satoru Akimoto, Miki Horimoto & Taichi Usui (1997) Galactosyl Transfer onto p-
Nitrophenyl β-d-Glucoside Using β-d-Galactosidase from Bacillus civculans, Bioscience, Biotechnology, and Biochemistry,
61:7, 1118-1120, DOI: 10.1271/bbb.61.1118
To link to this article: http://dx.doi.org/10.1271/bbb.61.1118
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