Steroids p. 575 - 592 (1983)
Update date:2022-08-29
Topics:
Murata
Beppu
Takikawa
Otsuka
Kasama
Seyama
A Sep-Pak C18 cartridge was used for purification of bile acids from serum. Three kinds of deuterium labeled internal standards were required for accurate measurement of individual sulfated and nonsulfated bile acids. These internal standards were added to the serum before its application to the cartridge. Separation of sulfated and nonsulfated bile acids was performed on piperidinohydroxypropyl Sephadex LH-20 column chromatography. The nonsulfate fraction was submitted to alkaline hydrolysis, and the sulfate fraction to solvolysis followed by alkaline hydrolysis. Each fraction was converted to the hexafluoroisopropyl-trifluoroacetyl derivatives and quantitated by mass fragmentography. The recovery of each bile acid sulfate was quite satisfactory. In fasting healthy subjects the mean of total nonsulfated bile acids in serum was 1.324 μg/ml, and that of total sulfated bile acids was 0.450 μg/ml. Sulfated lithocholic aid comprised a large part of sulfated bile acids in healthy subjects.
View MoreContact:+86-22-83718541
Address:32th Floor, Rongqiao Center Intersection of Changjiang Road and Nankai Six Road Nankai District Tianjin 300102, China
Shanghai Yuantai Chemical Products Co., Ltd
Contact:021--66129803
Address:Chengyin Road,Shanghai,China
Chemtrade International ( China )
Contact:+86-532-86893005
Address:Rm 2-501, Huaxia Zonghe Building, No. 410 JInggangshan Road, Huangdao
Shandong Hongxiang Zinc Co., Ltd
Contact:086-0311-66187879
Address:DaWang developing zone
Puyang Huicheng Electronic Material Co., Ltd
website:http://huichengchem.weba.testwebsite.cn/index_en.html
Contact:+86-393-8910800
Address:West Section Shengli Road, Puyang457000, China
Doi:10.1039/j39680000245
(1968)Doi:10.1063/1.461916
(1992)Doi:10.1021/ja00767a059
(1972)Doi:10.1002/anie.201606796
(2016)Doi:10.1016/j.bmc.2017.12.018
(2018)Doi:10.1002/jhet.4144
(2020)