RESPONSES OF PHASEOLUS LUNATUS CALLUS TISSUES TO CYTOKININ-ACTIVE UREIDOPURINES

Article abstract of DOI:10.1016/S0031-9422(00)81566-6

Key Word Index - Phaseolus lunatus; Leguminosae; Fabaceae; lima beans; cytokinin; ureidopurines; N⁶-benzyladenine; Thidiazuron; plant tissue culture. Three ureidopurine derivatives were tested for their ability to promote the cytokinin-dependent growth of callus tissues derived from three genotypes of Phaseolus lunatus (cv.Jackson Wonder, cv.Kingston and P.I. 260415).In all three test systems, the ureidopurines exhibited the same relative cytokinin activities (6-phenylureidopurine > 6-p-tolylureidopurine > 6-cyclohexylureidopurine) and were less active than the aminopurine derivatives, N⁶-benzyladenine and N⁶-cyclohexylmethyladenine.Callus tissues of Jackson Wonder, which become cytokinin-autonomous if grown on on cytokinin-active urea drivatives, retained cytokinin-dependence on media containing optimal concentrations of ureidopurines.In this respect, the biological activity of the ureidopurines was similar to that of the aminopurine derivatives and differed from that of cytokinin-active urea derivatives tested in this system.