3,3',5,5'-Tetramethylbenzidine

Base Information Edit

Chemical Name:3,3',5,5'-Tetramethylbenzidine
CAS No.:54827-17-7
Molecular Formula:C16H20N2
Molecular Weight:240.348
Hs Code.:29215990
European Community (EC) Number:259-364-6
UNII:3B3T5CB8EO
DSSTox Substance ID:DTXSID5026120
Nikkaji Number:J338G
Wikipedia:3,3%27,5,5%27-Tetramethylbenzidine,3',5'-Tetramethylbenzidine
Wikidata:Q2259258
ChEMBL ID:CHEMBL1331128
Mol file:54827-17-7.mol

Synonyms:3,3',5,5'-tetramethylbenzidine;3,3',5,5'-TMP;tetramethyl benzidine;tetramethylbenzidine dihydrochloride

Suppliers and Price of 3,3',5,5'-Tetramethylbenzidine

Supply Marketing:Edit

Business phase:: The product has achieved commercial mass production^*data from LookChem market partment

Manufacturers and distributors:

Manufacture/Brand
Chemicals and raw materials
Packaging
price

TRC
3,3'',5,5''-Tetramethylbenzidine
10g
$ 155.00

TCI Chemical
3,3',5,5'-Tetramethylbenzidine >98.0%(HPLC)(T)
5g
$ 122.00

TCI Chemical
3,3',5,5'-Tetramethylbenzidine >98.0%(HPLC)(T)
1g
$ 37.00

TCI Chemical
3,3',5,5'-Tetramethylbenzidine [for Biochemical Research] >98.0%(HPLC)(T)
1g
$ 59.00

TCI Chemical
3,3',5,5'-Tetramethylbenzidine [for Biochemical Research] >98.0%(HPLC)(T)
5g
$ 161.00

TCI Chemical
3,3',5,5'-Tetramethylbenzidine >98.0%(HPLC)(T)
25g
$ 422.00

SynQuest Laboratories
3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine
100 g
$ 528.00

SynQuest Laboratories
3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine
50 g
$ 312.00

SynQuest Laboratories
3,3',5,5'-Tetramethylbiphenyl-4,4'-diamine
10 g
$ 93.00

Soltec Ventures
3,3',5,5'-Tetramethylbenzidene 5'-Tetramethylbenzidene
10gm
$ 120.00

Total 198 raw suppliers

Chemical Property of 3,3',5,5'-Tetramethylbenzidine Edit

Chemical Property:

Appearance/Colour:White or light yellow solid
Melting Point:168-171 °C(lit.)
Refractive Index:1.5519 (estimate)
Boiling Point:368.623 °C at 760 mmHg
PKA:4.49±0.10(Predicted)
Flash Point:210.8 °C
PSA：52.04000
Density:1.071 g/cm³
LogP:4.91400
Storage Temp.:2-8°C
Sensitive.:Light Sensitive
Solubility.:Slightly soluble. <0.1 g/100 mL at 20°C.
Water Solubility.:Slightly soluble.
XLogP3:3.6
Hydrogen Bond Donor Count:2
Hydrogen Bond Acceptor Count:2
Rotatable Bond Count:1
Exact Mass:240.162648646
Heavy Atom Count:18
Complexity:226

Purity/Quality:

99% ^*data from raw suppliers

3,3'',5,5''-Tetramethylbenzidine ^*data from reagent suppliers

Safty Information:

Pictogram(s): Xn, Xi
Hazard Codes:Xi,Xn
Statements: 36/38-36/37/38-22-41
Safety Statements: 26-36-36/37-39

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

Chemical Classes:Nitrogen Compounds -> Benzidine and Derivatives
Canonical SMILES:CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C
Description Tetramethylbenzidine(TMB) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA). It is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. Tetramethylbenzidine is degraded by sunlight and by fluorescent lights. It?is a soluble chromogen substrate for horseradish peroxidase detection systems. Tetramethylbenzidine is recommended for ELISA procedures.
Uses 3,3,5,5-Tetramethyl benzidine is used as a reagent in a sensitive staining procedure for the detection of low levels of heme-associated peroxidase activity of cytochrome P-450 on SDS-polyacrylamide or agarose gel; non-carcinogenic substitute for benzidine as reagent for the detection of blood and determination of hemoglobin content. 3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples and dextromethorphan and its major metabolite dextrorphan in urine samples using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).

Technology Process of 3,3',5,5'-Tetramethylbenzidine

There total 16 articles about 3,3',5,5'-Tetramethylbenzidine which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 96.0%

: 29418-31-3
1,2-Bis(2,4-dimethylphenyl)diazene

: 54827-17-7
3,3',5,5'-tetramethylbenzidine

Guidance literature:: With hydrogenchloride; zinc; In water; ethyl acetate; at 20 ℃;

Reference yield: 93.0%

: 24596-18-7
4-chloro-2,6-dimethylaniline

: 54827-17-7
3,3',5,5'-tetramethylbenzidine

Guidance literature:: With carbon dioxide; aluminium; 1-butyl-3-methylimidazolium trifluoromethanesulfonimide; at 45 ℃; for 10h; under 116262 Torr;
DOI:10.1039/c0jm02396e