Chemical Property of 4-Thiouridine
Chemical Property:
- Appearance/Colour:Light yellow crystalline
- Melting Point:139-140℃ (ethanol )
- Refractive Index:1.732
- PKA:4.75±0.20(Predicted)
- PSA：139.80000
- Density:1.72 g/cm3
- LogP:-1.48260
- Storage Temp.:−20°C
- Solubility.:DMSO (Slightly), Methanol (Slightly, Sonicated)
- Water Solubility.:Soluble in water (20 mg/ml), and methanol (10 mg/ml).
- XLogP3:-1.4
- Hydrogen Bond Donor Count:4
- Hydrogen Bond Acceptor Count:6
- Rotatable Bond Count:2
- Exact Mass:260.04669266
- Heavy Atom Count:17
- Complexity:374
- Purity/Quality:
-
99%, *data from raw suppliers
4-Thiouridine *data from reagent suppliers
Safty Information:
- Pictogram(s):
- Hazard Codes:
- Safety Statements:
24/25
- MSDS Files:
-
SDS file from LookChem
Total 1 MSDS from other Authors
Useful:
- Canonical SMILES:C1=CN(C(=O)NC1=S)C2C(C(C(O2)CO)O)O
- Isomeric SMILES:C1=CN(C(=O)NC1=S)[C@H]2[C@@H]([C@@H]([C@H](O2)CO)O)O
-
Description
4-Thiouridine (4-SU) is a photoactivatable ribonucleoside analog that is widely used for RNA analysis, including short-range RNA-RNA crosslinking and nascent RNA labeling. The crosslinking thio moiety is attached directly to the nucleotide base, thus 4-SU differs from uridine only by a single sulfur substitution. This offers the advantage of incorporating into an RNA chain with minimal structural perturbation and with similar base-pairing properties, reducing the likelihood that substitution will impair RNA interactions or activities.
-
Uses
Nucleotide analogue, essential for cell growth in certain bacterial species. 4-THIOURIDINE is also able to chelate with certain metal ions, and in tRNA it can act as a built-in antiphotomutagenic agent that protects Escherichia coli cells against mutagenesis. 4-Thiouridine is a photoreactive (crosslinking) uridine analogue that upon phosphorylation to 4-thioUTP may be incorporated into RNA structures. 4-Thiouridine may be used to study the specificity and kinetics of uridine-cytidine kinases. 4-Thiouridine is a constituent of several transfer RNA's. 4-Thiouridine is a thionucleobase used as an antisense agent. Widely used for RNA analysis including RNA-RNA cross coupling and RNA labelling. 4-Thiouridine is a photoreactive (crosslinking) uridine analogue that upon phosphorylation to 4-thioUTP may be incorporated into RNA structures. 4-Thiouridine may be used to study the specificity and kinetics of uridine-cytidine kinases. 4-Thio Uridine is typically used to modify oligos slated for RNA, or RNA-protein, structural studies. A 4-thio-rU modified RNA pentamer was used to study the effect of this modification on codon-anticodon interaction when it is in the wobble position of tRNA.
