Chemical Property of Monobromobimane
Chemical Property:
- Vapor Pressure:0.000197mmHg at 25°C
- Melting Point:152-154oC
- Boiling Point:327.8°C at 760 mmHg
- PKA:-3.36±0.70(Predicted)
- Flash Point:152.1°C
- PSA：42.96000
- Density:1.66g/cm3
- LogP:1.01710
- Storage Temp.:2-8°C
- Solubility.:DMF: soluble
- XLogP3:1.4
- Hydrogen Bond Donor Count:0
- Hydrogen Bond Acceptor Count:3
- Rotatable Bond Count:1
- Exact Mass:270.00039
- Heavy Atom Count:15
- Complexity:437
- Purity/Quality:
-
99%, *data from raw suppliers
Monobromobimane *data from reagent suppliers
Safty Information:
- Pictogram(s):
- Hazard Codes:
- Safety Statements:
22-24/25
- MSDS Files:
-
SDS file from LookChem
Total 1 MSDS from other Authors
Useful:
- Canonical SMILES:CC1=C(N2C(=C(C(=O)N2C1=O)C)CBr)C
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Description
Monobromobimane is a thiol-reactive fluorogenic probe. It is cell-permeable, reacts rapidly at physiological pH with available thiol groups, and generates a stable fluorescent signal. Monobromobimane can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione, proteins, and nucleotides. The absorption and emission maxima for monobromobimane are 398 and 490 nm, respectively.
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Uses
Monobromobimane readily reacts with low molecular weight thiols. Bimanes are useful for detecting the distribution of protein thiols in cells before and after chemical reduction of disulfides.
Fluorescence: max. Abs. 398nm; e x 10-3: 5.0 Monobromobimane is essentially nonfluorescent until conjugated to target molecule. It readily reacts with several low molecular weight thiols, including glutathione, mercaptopurine, peptides and plasma thiols, as well as with carboxylic acids. double labeled product that can be fixed with aldehydes for cell-lineage tracing1, neuronal tracing2 and transplantation3. Monobromobimane is a thiol-reactive fluorogenic probe. It is cell-permeable, reacts rapidly at physiological pH with available thiol groups, and generates a stable fluorescent signal. Monobromobimane can be used to evaluate or quantify a variety of compounds containing reactive sulfur or thiol groups, including H2S, glutathione, proteins, and nucleotides. The absorption and emission maxima for monobromobimane are 398 and 490 nm, respectively.
