Guanidin-ium-isothiocyanate

Base Information

• Chemical Name: Guanidin-ium-isothiocyanate
• CAS No.: 593-84-0
• Molecular Formula: C2H6N4S
• Molecular Weight: 118.162
• Hs Code.: 29252000
• Mol file: 593-84-0.mol

Synonyms:guanidin-ium-isothiocyanate;salt guanidinium thiocyanate;SCHEMBL56026

Chemical Property of Guanidin-ium-isothiocyanate

Chemical Property:

• Appearance/Colour: White cryst. powder
• Vapor Pressure: 0.00206mmHg at 25°C
• Melting Point: 120-122 °C(lit.)
• Refractive Index: n20/D 1.482
• Boiling Point: 132.9 °C at 760 mmHg
• PKA: 12.5[at 20 ℃]
• Flash Point: 34.2 °C
• PSA: 138.48000
• Density: 1.103 g/mL at 20 °C
• LogP: 0.73618
• Storage Temp.: Store at RT.
• Sensitive.: Light Sensitive
• Solubility.: H2O: 6 M at 20 °C, clear, colorless
• Water Solubility.: Soluble in water and ethanol
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 2
• Rotatable Bond Count: 0
• Exact Mass: 118.03131738
• Heavy Atom Count: 7
• Complexity: 50.1

Purity/Quality:

98%min ^*data from raw suppliers

Guanidine Thiocyanate ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xn, T
• Hazard Codes: Xn,T,C
• Statements: 20/21/22-32-52/53-36/37/38-34
• Safety Statements: 36/37-61-13-36-26-45-36/37/39

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: C(#N)[S-].C(=[NH2+])(N)N
• Description: Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• Uses: Guanidine thiocyanate is a potent protein denaturant (stronger than guanidine HCl) often used in the isolation of intact ribonucleic acid to eliminate RNase activity. RNase can recover activity after boiling, but is irreversibly inactivated in a 4 M solution of guanidine thiocyanate. Such solutions, to which the reducing agent b-mercaptoethanol is often added, are used to inactivate RNAse when isolating RNA from tissues that are rich in RNase, such as liver. Total nuclear and cytoplasmic RNA may be isolated this way. A protocol for extracting RNA with guanidine isothiocyanate has been published.In the presence of guanidine thiocyanate, proteins dissolve readily, cellular structures disintegrate and nucleoproteins dissociate from nucleic acids, as protein secondary structure is lost. Potent protein denaturant used in isolation of intact DNA, RNA. Guanidine thiocyanate is a useful tool for RNA isolation and protein solubilization. It is involved in the quantification of mRNA from hepatocytes. It acts as a medium for blood smaple lystae in order to maintain the integrity of nucleic acid. It is a chaotropic agent, mainly involved in the extraction of DNA and RNA. It is utilized to deactivate the influenza virus. Guanidine thiocyanate has been used as a component of the stabilization buffer for RNA lysis. It can be used for deactivation of endogenous RNases leading to denaturation of nucleoprotein complexes. It is also suitable for denaturation of macromolecules such as nucleic acids and proteins and for RNA isolation.

Technology Process of Guanidin-ium-isothiocyanate

There total 19 articles about Guanidin-ium-isothiocyanate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 90.0%

ammonium thiocyanate + dicyandiamide (127099-85-8,780722-26-1) → guanidinium thiocyanate (593-84-0,5341-59-3)

Guidance literature:

In melt; 55 part of dicyanodiamide reacted with 100 part of NH4SCN;

Reference yield:

ammonium thiocyanate → guanidinium thiocyanate (593-84-0,5341-59-3)

Guidance literature:

heating over temp. of formation of (NH4)2CS;

Reference yield:

ammonium thiocyanate → ammonium salt of trithiocarbonic acid + guanidinium thiocyanate (593-84-0,5341-59-3)

Guidance literature:

In neat (no solvent); heating to 160-185°C;

upstream raw materials:

2,4,6-triamino-s-triazine

ammonium thiocyanate

CYANAMID

N-Cyanoguanidine

Downstream raw materials:

1,8-Diguanidino-octan

N,N'''-1,10-decanediylbisguanidine

2,4-Diamino-6-ethoxy-5-cyan-pyrimidin

N-(4-acetoxy-phenyl)-N'-carbamimidoyl-thiourea

Refernces