N-BUTYLFLUORESCEIN

Base Information

• Chemical Name: N-BUTYLFLUORESCEIN
• CAS No.: 335193-91-4
• Molecular Formula: C24H20O5
• Molecular Weight: 388.41
• Mol file: 335193-91-4.mol

Synonyms:N-BUTYLFLUORESCEIN;3'-Butoxy-6'-hydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one

Chemical Property of N-BUTYLFLUORESCEIN

Chemical Property:

• Melting Point: 203-205°C
• Boiling Point: 603.7oC at 760 mmHg
• Flash Point: 212.9oC
• PSA: 64.99000
• Density: 1.38g/cm3
• LogP: 5.13910
• Storage Temp.: -20?C Freezer

Purity/Quality:

95% ^*data from raw suppliers

N-Butylfluorescein ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Useful:

• Description: N-Butylfluorescein is a fluorescent compound that displays excitation/emission maxima of 467/512 nm, respectively. It has been used in the synthesis of butyl fluorescein myo-inositol phosphate (butyl FLIP), a fluorogenic substrate of phosphatidylinositol-specific phospholipase C (PI-PLC).
• Uses: A fluorogenic substrate

Technology Process of N-BUTYLFLUORESCEIN

There total 2 articles about N-BUTYLFLUORESCEIN which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

fluorescein 3-O-butyl ether n-butyl ester (131205-61-3) → 3'-O-butylfluorescein (335193-91-4)

Guidance literature:

With sodium hydroxide; In methanol; water; for 2h;

DOI:10.1016/j.bmc.2003.11.016

Reference yield:

fluorescein disodium salt (518-47-8) → 3'-O-butylfluorescein (335193-91-4)

Guidance literature:

Multi-step reaction with 2 steps

1: dimethylformamide / 24 h / 90 - 100 °C

2: 1.23 g / NaOH / methanol; H₂O / 2 h

With sodium hydroxide; In methanol; water; N,N-dimethyl-formamide;

DOI:10.1016/j.bmc.2003.11.016

Reference yield:

(R)-2-(2,5-Dioxo-2,5-dihydro-pyrrol-1-yl)-3,3-diphenyl-propionyl chloride + 3'-O-butylfluorescein (335193-91-4) → 6'-O-(N-maleimido-D-3,3-diphenylalanyl)-3'-O-butylfluorescein (676362-51-9)

Guidance literature:

With triethylamine; In dichloromethane; at 0 - 20 ℃;

DOI:10.1016/j.bmc.2003.11.016

upstream raw materials:

fluorescein 3-O-butyl ether n-butyl ester

fluorescein disodium salt

Downstream raw materials:

6'-O-(N-maleimido-D-3,3-diphenylalanyl)-3'-O-butylfluorescein

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