518-47-8 Usage
Description
Fluorescein disodium salt, also known as a sodium salt of fluorescein, is an orange-red to dark red powder that is odorless and almost tasteless. It is a water-soluble form of fluorescein and may be sensitive to prolonged exposure to light and heat. Its chemical properties include being hygroscopic, soluble in water, and sparingly soluble in alcohol.
Uses
Used in Environmental Testing:
Fluorescein disodium salt is used as a tracer for examining subterranean waters, helping to ascertain the source of springs, connections between streams and seas, determine the approximate volume of water delivered by a spring, and detect the source of contamination in drinking water, as well as the infiltration of soil with waste waters from factories.
Used in Medicine:
Fluorescein disodium salt is used as an analytical reagent for protein labeling and as a clinical reagent for immuno-histological staining and immuno-fluorescent labeling.
Used in Cosmetics:
It is used in externally applied drugs and cosmetics for shading purposes.
Used in Technical Applications:
Fluorescein sodium salt can be used in the in-situ photochemical fabrication of transition metal-promoted amorphous molybdenum sulfide catalysts for enhanced photosensitized hydrogen evolution.
Used in Aerial Reconnaissance:
Its brilliant yellow color and strong green fluorescence when dissolved in water and ethanol make it suitable for use in aerial reconnaissance search, as it helps in identifying targets with fluorescence.
Used in Groundwater Exploration:
Fluorescein disodium salt can also be used significantly for the exploration of fluorescent groundwater.
Used in Silk Printing and Dyeing:
Although rarely, it can be used for silk printing and dyeing purposes.
Air & Water Reactions
Hygroscopic. Water soluble.
Reactivity Profile
Fluorescein disodium salt is incompatible with strong oxidizers. Also incompatible with acids, acid salts and salts of heavy metals .
Fire Hazard
Flash point data for Fluorescein disodium salt are not available; however, Fluorescein disodium salt is probably combustible.
Safety Profile
Moderately toxic by
intraperitoneal route. Mildly toxic by
ingestion. Human systemic effects by
intravenous route: arrhythmias, eye
hemorrhage, nausea or vomiting.
Experimental reproductive effects.
Questionable carcinogen with experimental
tumorigenic data. Mutation data reported.
When heated to decomposition it emits
toxic fumes of Na2O.
Veterinary Drugs and Treatments
Fluorescein sodium is a yellow water-soluble dye that fluoresces
under a Wood’s Lamp, but is plainly visible after binding to corneal
stroma through an ophthalmic examination light source. It is
used most commonly to delineate full thickness loss of corneal epithelium
indicating the presence of a corneal ulceration. In this instance
it will stain the corneal stroma. The epithelium is not stained
because its outer lipid cell membrane repels the stain. Descemet’s
membrane will not stain with fluorescein stain and this is used to
indicate descemetocele formation, an ocular emergency.
Fluorescein stain is applied to the precorneal tear film in dogs
and cats and the break-up of this stain with time, as observed
through a slit lamp biomicroscope using a cobalt blue light source,
is used to determine the tear film break-up time (normal 19 seconds),
an indicator of tear film quality.
Fluorescein stain is applied to the tear film of dogs to determine
patency of the nasolacrimal outflow system. The normal wait time
is 2 – 5 minutes in dogs and up to 10 minutes in cats. A positive test
indicates patency of the system. A negative test is not indicative of
disease as the test is negative in a large percentage of normal animals.
Fluorescein stain, then, can be added to irrigating solution to
flush the nasolacrimal system, making detecting the irrigation solution
at the nose more obvious during flushing of the system.
Check Digit Verification of cas no
The CAS Registry Mumber 518-47-8 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,1 and 8 respectively; the second part has 2 digits, 4 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 518-47:
(5*5)+(4*1)+(3*8)+(2*4)+(1*7)=68
68 % 10 = 8
So 518-47-8 is a valid CAS Registry Number.
InChI:InChI=1/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1
518-47-8Relevant articles and documents
A highly specific fluorescent probe for hypochlorous acid and its application in imaging microbe-induced hocl production
Xu, Qingling,Lee, Kyung-Ah,Lee, Songyi,Lee, Kyung Mi,Lee, Won-Jae,Yoon, Juyoung
, p. 9944 - 9949 (2013)
Oxidative stress induced by reactive oxygen species (ROS) plays crucial roles in a wide range of physiological processes and is also implicated in various diseases, including cancer, chronic inflammatory diseases, and neurodegenerative disorders. Among the various ROS, hypochlorous acid (HOCl) plays as a powerful microbicidal agent in the innate immune system. The regulated production of microbicidal HOCl is required for the host to control the invading microbes. However, as a result of the highly reactive and diffusible nature of HOCl, its uncontrolled production may lead to an adverse effect on host physiology. Because of its biological importance, many efforts have been focused on developing selective fluorescent probes to image ROS. However, it is still challenging to design a fluorescent probe with exclusive selectivity toward a particular member of ROS. In the current work, we designed FBS as a new fluorescent HOCl probe which has high selectivity, sensitivity, and short response time in a broad range of pH. Compared with other sensors, the dual-lock structure of FBS has an advantage of eliminating interferences from other ROS/RNS. Importantly, we further showed that our HOCl probe could be applied for the in vivo imaging of physiological HOCl production in the mucosa of live animals. This probe provides a promising tool for the study of HOCl production.