Thiazolyl Blue

Base Information

• Chemical Name: Thiazolyl Blue
• CAS No.: 298-93-1
• Deprecated CAS: 117419-96-2,117830-92-9,133683-46-2,156758-88-2,2348-71-2,3568-69-2,61357-96-8,74722-46-6,85556-98-5,93550-34-6,460315-48-4,879640-77-4,2320733-47-7,2611300-77-5
• Molecular Formula: C18H16BrN5S
• Molecular Weight: 414.329
• Hs Code.: 29341000
• European Community (EC) Number: 206-069-5
• NSC Number: 60102,367079
• UNII: EUY85H477I
• DSSTox Substance ID: DTXSID60889338
• Wikidata: Q27124034
• Metabolomics Workbench ID: 58598
• Mol file: 298-93-1.mol

Synonyms:2H-Tetrazolium,2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (8CI,9CI);3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazoliumbromide (6CI,7CI);(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide);2,5-Diphenyl-3-(4,5-dimethylthiazol-2-yl)-2H-tetrazolium bromide;2-(4,5-Dimethylthiazol-2-yl)-3,5-diphenyl-2H-tetrazolium bromide;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide;3-(4,5-Dimethylthiazolyl)-2,5-diphenyltetrazolium bromide;MMTTetrazolium;MTT;MTT tetrazolium;NSC 60102;Thiazolyl blue (Sigma);Thiazolyl blue tetrazolium bromide;

Chemical Property of Thiazolyl Blue

Chemical Property:

• Appearance/Colour: yellow crystalline powder
• Melting Point: 195 °C (dec.)(lit.)
• PSA: 75.72000
• Density: 1.4886 (estimate)
• LogP: 0.28830
• Storage Temp.: 2-8°C
• Sensitive.: Light Sensitive
• Solubility.: methanol: 5 mg/mL, clear
• Water Solubility.: Slightly soluble
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 5
• Rotatable Bond Count: 3
• Exact Mass: 413.03098
• Heavy Atom Count: 25
• Complexity: 410

Purity/Quality:

98.0% ^*data from raw suppliers

Thiazolyl Blue Tetrazolium Bromide ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi, T
• Hazard Codes: Xi,T,Xn
• Statements: 46-68-36/37/38
• Safety Statements: 22-24/25-53-45-36/37-26

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Chemical Classes: Dyes -> Azo Dyes
• Canonical SMILES: CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=CC=C3)C4=CC=CC=C4)C.[Br-]
• Recent EU Clinical Trials: Long Term Administration of Inahled Mannitol in Cystic Fibrosis - A Safety and Efficacy Study
• Uses: Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Technology Process of Thiazolyl Blue

There total 1 articles about Thiazolyl Blue which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield:

→ thiazolyl blue (298-93-1,2348-71-2)

Guidance literature:

With N-Bromosuccinimide; ethyl acetate;

Reference yield:

ammonium metavanadate + thiazolyl blue (298-93-1,2348-71-2) + 5-methyl-4-(2-thiazolylazo)resorcinol (37422-56-3) → 2O2V(1+)*2C10H7N3O2S(2-)*2C18H16N5S(1+)

Guidance literature:

In chloroform; water;

DOI:10.5562/cca2436

Reference yield:

thiazolyl blue (298-93-1,2348-71-2) → 1-(4,5-dimethyl-2-thiazoyl)-3,5-diphenylformazan (23305-68-2)

Guidance literature:

With dehydrogenase;

DOI:10.14233/ajchem.2014.16930

Downstream raw materials:

1-(4,5-dimethyl-2-thiazoyl)-3,5-diphenylformazan