Technology Process of all-trans-retinaldoxime
There total 4 articles about all-trans-retinaldoxime which
guide to synthetic route it.
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synthetic route:
- Guidance literature:
-
all-trans-Retinal;
With
NAD; NADP; ATP; 2-amino-2-hydroxymethyl-1,3-propanediol; Sucrose;
glycosylation-deficient HEK-293S cells transfected with mouse retinal dehydrogenase type 1 (HEK-RALDH1 cells);
at 37 ℃;
for 2h;
pH=8.0;
Enzymatic reaction;
With
hydroxylamine;
In
water; acetonitrile;
at 20 ℃;
for 0.5h;
pH=7.0;
With
ammonium acetate;
In
water;
pH=4.0;
Product distribution / selectivity;
- Guidance literature:
-
Multi-step reaction with 2 steps
1.1: NAD; NADP; 2-amino-2-hydroxymethyl-1,3-propanediol; bovine serum albumin / equine liver alcohol dehydrogenases (ADH) (EC 1.1.1.1 [EC] ) / N,N-dimethyl-formamide / 1 h / 37 °C / pH 8.8 / Enzymatic reaction
2.1: NAD; NADP; ATP; 2-amino-2-hydroxymethyl-1,3-propanediol; Sucrose / glycosylation-deficient HEK-293S cells / 2 h / 37 °C / pH 8.0 / Enzymatic reaction
2.2: 0.5 h / 20 °C / pH 7.0
2.3: pH 4.0
With
NAD; NADP; ATP; 2-amino-2-hydroxymethyl-1,3-propanediol; bovine serum albumin; Sucrose;
equine liver alcohol dehydrogenases (ADH) (EC 1.1.1.1 [EC] ); glycosylation-deficient HEK-293S cells;
In
N,N-dimethyl-formamide;
- Guidance literature:
-
RETINOL;
With
NAD; NADP; 2-amino-2-hydroxymethyl-1,3-propanediol; bovine serum albumin;
equine liver alcohol dehydrogenases (ADH) (EC 1.1.1.1 [EC] );
In
N,N-dimethyl-formamide;
at 37 ℃;
for 1h;
pH=8.8;
Enzymatic reaction;
With
hydroxylamine;
In
methanol; water; N,N-dimethyl-formamide;
at 20 ℃;
for 0.25h;
pH=7.0;
Product distribution / selectivity;