2,3,5-Triphenyltetrazolium chloride

Base Information

• Chemical Name: 2,3,5-Triphenyltetrazolium chloride
• CAS No.: 298-96-4
• Deprecated CAS: 80949-00-4,52230-05-4
• Molecular Formula: C19H15N4Cl
• Molecular Weight: 334.808
• Hs Code.: 29339900
• European Community (EC) Number: 206-071-6
• NSC Number: 5053
• UNII: D25727K0RB
• DSSTox Substance ID: DTXSID30889340
• Wikipedia: Tetrazolium_chloride
• Wikidata: Q415248
• Metabolomics Workbench ID: 67576
• ChEMBL ID: CHEMBL401540
• Mol file: 298-96-4.mol

Synonyms:2,3,5-triphenyltetrazolium chloride;triphenyltetrazolium;triphenyltetrazolium bromide;triphenyltetrazolium chloride

Chemical Property of 2,3,5-Triphenyltetrazolium chloride

Chemical Property:

• Appearance/Colour: white powder
• Vapor Pressure: 0.0615mmHg at 25°C
• Melting Point: 250 °C(lit.)
• Refractive Index: 1.6110 (estimate)
• Boiling Point: >360
• Flash Point: 91.7oC
• PSA: 34.59000
• Density: 1.1704 (rough estimate)
• LogP: 0.21500
• Storage Temp.: 2-8°C
• Sensitive.: Light Sensitive & Hygroscopic
• Solubility.: H2O: may be clear to slightly hazy
• Water Solubility.: Soluble in water, ethanol and acetone. Insoluble in ether.
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 3
• Rotatable Bond Count: 3
• Exact Mass: 334.0985242
• Heavy Atom Count: 24
• Complexity: 330

Purity/Quality:

99% ^*data from raw suppliers

2,3,5-Triphenyltetrazolium Chloride ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xi,Xn
• Hazard Codes: Xi,Xn
• Statements: 36/37/38-20/21/22
• Safety Statements: 26-36

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Chemical Classes: Dyes -> Other Dyes
• Canonical SMILES: C1=CC=C(C=C1)C2=NN([N+](=N2)C3=CC=CC=C3)C4=CC=CC=C4.[Cl-]
• Recent ClinicalTrials: Comparison of Electrical Pudendal Nerve Stimulation and Tolterodine Tartrate for Urgency-Frequency Syndrome in Women
• General Description: 2,3,5-Triphenyltetrazolium chloride (TTC) is a redox-sensitive dye commonly used as an indicator of metabolic activity in biological systems, particularly in assessing cell viability and mitochondrial function. It is a colorless compound that is enzymatically reduced to formazan, a red-colored product, in the presence of viable cells with active electron transport chains. TTC is widely applied in microbiology, histochemistry, and plant physiology to distinguish between living and non-living tissues. Its synonyms include Tetrazolium red, Vitastain, and Uroscreen, reflecting its utility in various diagnostic and staining applications. 2,3,5-Triphenyltetrazolium chloride’s ability to detect cellular respiration makes it valuable in studies of ischemia, cytotoxicity, and seed viability testing.

Technology Process of 2,3,5-Triphenyltetrazolium chloride

There total 9 articles about 2,3,5-Triphenyltetrazolium chloride which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 95.0%

1,3,5-triphenylformazan (531-52-2) → triphenyltetrazolium chloride (298-96-4)

Guidance literature:

With thallium(III) acetate; In acetic acid;

Reference yield: 88.0%

1,3,5-triphenylformazan (49624-20-6) → triphenyltetrazolium chloride (298-96-4)

Guidance literature:

With N-chlorobenzotriazole; In benzene; for 0.25h; Heating;

Reference yield:

tert-butylhypochlorite (507-40-4) + 1,3,5-triphenylformazan (531-52-2) → triphenyltetrazolium chloride (298-96-4)

Guidance literature:

With potassium hydroxide; chloroform;

DOI:10.1021/ja01635a070

upstream raw materials:

N-chloro-succinimide

1,3,5-triphenylformazan

N-chlorophthalimide

tert-butylhypochlorite

Downstream raw materials:

2,3,5-triphenyl-tetrazolium; hydroxide

2-phenyl-benzo[c]tetrazolo[2,3-a]cinnolinylium; chloride

2,3,5-triphenyl-tetrazolium; triiodide

N′-phenylbenzimidohydrazide