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5,5'-Dithiobis(2-nitrobenzoic acid)

Base Information Edit
  • Chemical Name:5,5'-Dithiobis(2-nitrobenzoic acid)
  • CAS No.:69-78-3
  • Molecular Formula:C14H8N2O8S2
  • Molecular Weight:396.358
  • Hs Code.:29309070
  • European Community (EC) Number:200-714-4
  • UNII:9BZQ3U62JX
  • DSSTox Substance ID:DTXSID5058779
  • Nikkaji Number:J4.870D,J2.332.125D
  • Wikipedia:Ellman%27s_reagent,Ellman's_reagent
  • Wikidata:Q3604488
  • ChEMBL ID:CHEMBL395814
  • Mol file:69-78-3.mol
5,5'-Dithiobis(2-nitrobenzoic acid)

Synonyms:5,5'-Dithiobis(2-nitrobenzoic Acid);5,5'-Dithiobis(nitrobenzoate);Acid, Dithionitrobenzoic;Dithionitrobenzoic Acid;DTNB;Ellman Reagent;Ellman's Reagent;Ellmans Reagent;Reagent, Ellman's

Suppliers and Price of 5,5'-Dithiobis(2-nitrobenzoic acid)
Supply Marketing:Edit
Business phase:
The product has achieved commercial mass production*data from LookChem market partment
Manufacturers and distributors:
  • Manufacture/Brand
  • Chemicals and raw materials
  • Packaging
  • price
  • Usbiological
  • DTNB
  • 100ul
  • $ 499.00
  • TRC
  • 3,3''-Dithiobis[6-nitrobenzoicAcid]
  • 5g
  • $ 135.00
  • TCI Chemical
  • 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups] >98.0%(HPLC)(T)
  • 5g
  • $ 66.00
  • TCI Chemical
  • 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups] >98.0%(HPLC)(T)
  • 1g
  • $ 25.00
  • TCI Chemical
  • 5,5'-Dithiobis(2-nitrobenzoic Acid) [for Determination of SH groups] >98.0%(HPLC)(T)
  • 25g
  • $ 224.00
  • SynQuest Laboratories
  • 5,5'-Dithiobis(2-nitrobenzoic acid)
  • 25 g
  • $ 184.00
  • SynQuest Laboratories
  • 5,5'-Dithiobis(2-nitrobenzoic acid)
  • 1 g
  • $ 16.00
  • SynQuest Laboratories
  • 5,5'-Dithiobis(2-nitrobenzoic acid)
  • 5 g
  • $ 40.00
  • Soltec Ventures
  • 5,5′-Dithiobis(2-nitrobenzoic acid) 5'-Dithio-bis(2-nitrobenzoicacid)
  • 5gm
  • $ 92.00
  • Sigma-Aldrich
  • 5,5′-Dithiobis(2-nitrobenzoic acid) BioReagent, ≥98%, suitable for determination of sulfhydryl groups
  • 25g
  • $ 360.00
Total 120 raw suppliers
Chemical Property of 5,5'-Dithiobis(2-nitrobenzoic acid) Edit
Chemical Property:
  • Appearance/Colour:Yellow solid. 
  • Vapor Pressure:5.73E-19mmHg at 25°C 
  • Melting Point:240-245 °C (dec.)(lit.) 
  • Refractive Index:1.6900 (estimate) 
  • Boiling Point:671.863 °C at 760 mmHg 
  • PKA:1.57±0.25(Predicted) 
  • Flash Point:360.13 °C 
  • PSA:216.84000 
  • Density:1.787 g/cm3 
  • LogP:4.74520 
  • Storage Temp.:Store at RT. 
  • Solubility.:Do you have solubility information on this product that you woul 
  • Water Solubility.:Soluble in water and ethanol. 
  • XLogP3:2.7
  • Hydrogen Bond Donor Count:2
  • Hydrogen Bond Acceptor Count:10
  • Rotatable Bond Count:5
  • Exact Mass:395.97220756
  • Heavy Atom Count:26
  • Complexity:528
Purity/Quality:

98%, *data from raw suppliers

DTNB *data from reagent suppliers

Safty Information:
  • Pictogram(s): IrritantXi 
  • Hazard Codes:Xi 
  • Statements: 36/37/38 
  • Safety Statements: 26-36 
MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:
  • Chemical Classes:Dyes -> Indicator Dyes
  • Canonical SMILES:C1=CC(=C(C=C1SSC2=CC(=C(C=C2)[N+](=O)[O-])C(=O)O)C(=O)O)[N+](=O)[O-]
  • Uses 5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample. Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content. 5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups.
Technology Process of 5,5'-Dithiobis(2-nitrobenzoic acid)

There total 9 articles about 5,5'-Dithiobis(2-nitrobenzoic acid) which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:
Guidance literature:
With sodium sulfide; In water; at 50 ℃; for 2h; pH=> 3.5;
Guidance literature:
5-thio-2-nitrobenzoic acid; With supramolecular artificial glutathione peroxidase (SGPxmax); In aq. phosphate buffer; at 36 ℃; for 0.05h; pH=7; Enzymatic reaction;
Cumene hydroperoxide; In aq. phosphate buffer; Reagent/catalyst; Kinetics; Catalytic behavior;
DOI:10.1039/c4ra04042b
Guidance literature:
With potassium hydroxide; dihydrogen peroxide; In water;
DOI:10.1021/ja00543a028
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