Chemical Property of 5-Carboxyfluorescein
Chemical Property:
- Appearance/Colour:Orange solid
- Vapor Pressure:4.5E-22mmHg at 25°C
- Melting Point:368-372°C
- Refractive Index:1.816
- Boiling Point:725.2 °C at 760 mmHg
- PKA:3.3, 4.6, 6.4, 7.0(at 25℃)
- Flash Point:266.9 °C
- PSA：113.29000
- Density:1.73 g/cm3
- LogP:3.36400
- Storage Temp.:Store at 2-8
- Solubility.:DMF: soluble
- XLogP3:2.9
- Hydrogen Bond Donor Count:3
- Hydrogen Bond Acceptor Count:7
- Rotatable Bond Count:1
- Exact Mass:376.05830272
- Heavy Atom Count:28
- Complexity:637
- Purity/Quality:
-
99% *data from raw suppliers
5-Carboxyfluorescein *data from reagent suppliers
Safty Information:
- Pictogram(s):
Xi
- Hazard Codes:Xi
- Statements:
36
- Safety Statements:
26-39
- MSDS Files:
-
SDS file from LookChem
Useful:
- Canonical SMILES:C1=CC2=C(C=C1C(=O)O)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
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Description
5-Carboxyfluorescein is a single isomer derivative of 5(6)-carboxyfluorescein that can be used to fluorescently label biomolecules through the interaction of carboxylic acid with primary amines. It demonstrates excitation/emission maxima of 492 and 518 nm, respectively.
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Uses
5-Carboxyfluorescein(5-FAM) contains a carboxylic acid that can be used to react with primary amines via carbodiimide activation of the carboxylic acid. It is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. It has been principally used to develop a variety of green fluorescent reagents and small fluorescent molecules due to its relatively high absorbance characteristics, excellent fluorescence quantum yield, and good water solubility. The probe has also found application in studies of liposome-cell, cell-cell, and liposome-liposome interactions and in related studies on lipid bilayer structures.
