L-Glutamine-amide-15N

Base Information

• Chemical Name: L-Glutamine-amide-15N
• CAS No.: 59681-32-2
• Molecular Formula: C5H10N2O3
• Molecular Weight: 147.15
• DSSTox Substance ID: DTXSID00574155
• Nikkaji Number: J346.712K
• Wikidata: Q82463094
• Mol file: 59681-32-2.mol

Synonyms:L-Glutamine-amide-15N;59681-32-2;L-Glutamine-15N-1;(2S)-2-amino-5-(15N)azanyl-5-oxopentanoic acid;Acustasin-15N;Aesgen 14-15N;L-(N~5~-~15~N)Glutamine;L-Glutamine-(amide-15N);L-Glutamine-amide-(1)?N;DTXSID00574155;HY-N0390S9;MS-22834;CS-0376600;L-Glutamine-(amide-15N), 98 atom % 15N

Chemical Property of L-Glutamine-amide-15N

Chemical Property:

• Melting Point: 185 °C (dec.)(lit.)
• PSA: 106.41000
• LogP: 0.06440
• Storage Temp.: Room Temperature
• Solubility.: Aqueous Acid (Very Slightly), Water (Slightly)
• XLogP3: -3.1
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 4
• Exact Mass: 147.06617708
• Heavy Atom Count: 10
• Complexity: 146

Purity/Quality:

98% by HPLC; 98% atom 15N ^*data from raw suppliers

L-Glutamine-amide-15N ^*data from reagent suppliers

Safty Information:

• Pictogram(s):  
• Hazard Codes: 

MSDS Files:

SDS file from LookChem

Total 1 MSDS from other Authors

Useful:

• Canonical SMILES: C(CC(=O)N)C(C(=O)O)N
• Isomeric SMILES: C(CC(=O)[15NH2])[C@@H](C(=O)O)N
• Uses: (L-Glutamine-amide-15N) Labelled Gluamine. L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Technology Process of L-Glutamine-amide-15N

There total 1 articles about L-Glutamine-amide-15N which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 95.0%

L-glutamic acid (56-86-0,21675-62-7,23009-64-5,25104-13-6,84960-48-5,25513-46-6) → <15N>-L-glutamine(amide 15N) (59681-32-2)

Guidance literature:

With ammonium-¹⁵N hydrochloride; adenosine 5'-triphosphate disodium salt; magnesium chloride; In water; at 38 ℃; for 24h; glutamine synthetase (GS, E_.C_. 6.3.1.2.), pH 7.1;

DOI:10.1002/recl.19941130706

Reference yield:

L-glutamine phosphate + <15N>-L-glutamine(amide 15N) (59681-32-2) → L-glutamine (56-85-9,26700-71-0) + C5H9N(15)NO6P(2-)

Guidance literature:

With L-glutamine kinase Cj₁₄₁₈; potassium chloride; magnesium chloride; In aq. buffer; at 25 ℃; for 12h;

DOI:10.1021/acs.biochem.8b00811

Reference yield:

13C5-15N2-L-glutamine (59681-32-2,80143-57-3,184161-19-1,159680-32-7,204451-48-9,494783-18-5) → 1,2,3,4,5-(13)C5,(15)N-L-glutamic acid (108395-12-6,1202063-54-4,206983-94-0,95388-01-5)

Guidance literature:

With glutaminase; 6-diazo-5-oxo-L-norleucine; In water; at 4 ℃; for 0.333333h; Enzymatic reaction;

DOI:10.1021/acschembio.6b00493

upstream raw materials:

L-glutamic acid

Downstream raw materials:

L-glutamine

1,2,3,4,5-⁽¹³⁾C₅,⁽¹⁵⁾N-L-glutamic acid