The choice of solvent in the reaction medium can have a profound effect on the enzymatic hydrolysis of diesters. Enzymes like lipases often exhibit different activity and selectivity in different solvents. For example, lipases are generally more active in nonpolar organic solvents like hexane or toluene. In contrast, they may have reduced activity in polar solvents or aqueous media. Therefore, the solvent choice should be tailored to the specific lipase and substrate.
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cis-1-(acetoxymethyl)-2-(hydroxymethyl)cyclohexane
5-methyl-3-phenyldihydrofuran-2(3H)-one
