Inhibition studies involve testing the synthesized compounds in vitro to assess their ability to inhibit the activity of enoyl ACP reductase. This is typically done through enzyme assays where the enzyme activity is measured in the presence of varying concentrations of the novel diphenyl ethers. IC50 values, representing the concentration of the compound needed to inhibit 50% of the enzyme activity, can be determined.
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3-hydroxy-4-phenoxy-benzoic acid
