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| CAS No.: | 298-83-9 |
|---|---|
| Name: | Nitrotetrazolium blue chloride |
| Molecular Structure: | |
|
|
|
| Formula: | C40H30N10O6.2Cl |
| Molecular Weight: | 817.648 |
| Synonyms: | 2H-Tetrazolium,2,2'-(3,3'-dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis[3-(4-nitrophenyl)-5-phenyl-,dichloride (9CI);Tetrazolium,3,3'-(3,3'-dimethoxy-4,4'-biphenylylene)bis[2-(p-nitrophenyl)-5-phenyl-2H-chloride](6CI);2,2'-Bis(p-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazoliumchloride;2,2'-Dinitrophenyl-5,5'-diphenyl-3,3'-dimethoxy-4,4'-diphenylene)ditetrazoliumchloride;3,3'-(3,3'-Dimethoxy-4,4'-diphenylene)bis[2-(p-nitrophenyl)-5-phenyltetrazoliumchloride];Nitro BT;Nitro Blue Tetrazolium chloride;Nitro Tetrazolium BT;Nitrotetrazolium blue;Tetrazolium nitro blue;p-Nitro blue tetrazolium; |
| EINECS: | 206-067-4 |
| Density: | 1.5521 (rough estimate) |
| Melting Point: | 200 °C |
| Solubility: | methanol: 50 mg/mL, clear, very strongly yellow |
| Appearance: | yellow crystals |
| Hazard Symbols: |
 Xn, Xi
|
| Risk Codes: | 22-68/20/21/22-20/21/22-36-36/37/38 |
| Safety: | 36-45-36/37-26 |
| PSA: | 179.28000 |
| LogP: | 1.29040 |
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Chemistry
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IUPAC Name: 2-[2-methoxy-4-[3-methoxy-4-[3-(4-nitrophenyl)-5-phenyltetrazol-2-ium-2-yl]phenyl]phenyl]-3-(4-nitrophenyl)-5-phenyltetrazol-2-ium dichloride
Molecular Formula: C40H30Cl2N10O6
Molecular Weight: 817.64 g/mol
EINECS: 206-067-4
Product Categories: Organics; Tetrazolium Salts; Tetrazolium Salts & Formazans
Classification Code: Indicators and reagents; Mutation data
Melting Point: 200 °C
storage temp.: 2-8 °C
solubility: methanol: 50 mg/mL, clear, very strongly yellow
Stability of NBT (CAS NO.298-83-9): Stable, but store cold.
Uses
NBT (CAS NO.298-83-9) is used in immunology for sensitive detection of alkaline phosphatase.
Toxicity Data With Reference
| Organism | Test Type | Route | Reported Dose (Normalized Dose) | Effect | Source |
|---|---|---|---|---|---|
| mouse | LD50 | oral | 2gm/kg (2000mg/kg) | Gekkan Yakuji. Pharmaceuticals Monthly. Vol. 32, Pg. 614, 1990. |
Consensus Reports
It is reported in EPA TSCA Inventory.
Safety Profile
Hazard Codes: Xn
,Xi
Risk Statements: 22-68/20/21/22-20/21/22-36-36/37/38
R20:Harmful by inhalation.
R68:Possible risk of irreversible effects.
R20/21/22:Harmful by inhalation, in contact with skin and if swallowed.
R36:Irritating to eyes.
R36/37/38:Irritating to eyes, respiratory system and skin.
Safety Statements: 36-45-36/37-26
S36:Wear suitable protective clothing.
S45:In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
S36/37:Wear suitable protective clothing and gloves.
S26: In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
WGK Germany: 3
RTECS: XF8045000
F: 8-10-23
HS Code: 32041900
Moderately toxic by ingestion. When heated to decomposition it emits toxic vapors of NOx and Cl−.
Specification
NBT (CAS NO.298-83-9), its Synonyms are Nitroblue tetrazolium ; Nitro BT ; Nitro Blue tetrazolium ; Nitro Blue tetrazolium chloride ; Nitro Blue tetrazolium salt ; Nitro tetrazolium BT ; Nitroblue tetrazolium salt ; Nitrotetrazolium Blue ; Nitrotetrazolium Chloride Blue ; Tetrazolium Nitro Blue ; Tetrazolium nitro BT ; p-Nitro Blue tetrazolium ; p-Nitro Blue tetrazolium chloride ; p-Nitrotetrazolium Blue ; 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(3-(4-nitrophenyl)-5-phenyl-, chloride (1:2) ; 2H-Tetrazolium, 2,2'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(3-(4-nitrophenyl)-5-phenyl-, dichloride ; 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2-(4-nitrophenyl)-5-phenyl-, dichloride ; 2H-Tetrazolium, 3,3'-(3,3'-dimethoxy(1,1'-biphenyl)-4,4'-diyl)bis(2-(4-nitrophenyl)-5-phenyl-,dichloride ; 5,5'-Diphenyl-3,3'-bis(4-nitrophenyl)-2,2'-(3,3'-dimethoxybiphenyl-4,4'-ylene)ditetrazolium dichloride . It is yellow crystals.