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Basic Information
CAS No.: 550-82-3
Name: RESAZURIN
Article Data: 11
Molecular Structure:
Molecular Structure of 550-82-3 (RESAZURIN)
Formula: C12H7 N O4
Molecular Weight: 229.192
Synonyms: Resazurin(6CI); 7-Hydroxy-3H-phenoxazin-3-one 10-oxide; 7-Hydroxy-3H-phenoxazine-3-one10-oxide; Alamar Blue; Azoresorcin; Diazoresorcinol; NSC 10438; NSC 56323;Resazoin; Resazurine; Rhodalux
Density: 1.57g/cm3
Melting Point: >250°C
Boiling Point: 477.2°Cat760mmHg
Flash Point: 242.4°C
Safety: Moderately toxic by ingestion. When heated to decomposition it emits toxic fumes of NOx.
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Chemistry

Molecule structure of 7-Hydroxy-3H-phenoxazin-3-one-10 oxide (CAS NO.550-82-3) :

IUPAC Name: 7-hydroxy-10-oxidophenoxazin-10-ium-3-one 
Molecular Weight: 229.18828 g/mol
Molecular Formula: C12H7NO4
Density: 1.57 g/cm3
Boiling Point: 477.2 °C at 760 mmHg
Flash Point: 242.4 °C  
Molar Volume: 145.7 cm3
Polarizability: 23.3*10-24 cm3
Surface Tension: 75.3 dyne/cm 
Enthalpy of Vaporization: 76.93 kJ/mol
Vapour Pressure: 9.94E-10 mmHg at 25 °C 
log P (octanol-water): 0.940 (none)
Atmospheric OH Rate Constant: 1.20E-10 cm3/molecule-sec at 25 °C
XLogP3-AA: -0.1
H-Bond Donor: 1
H-Bond Acceptor: 4
Tautomer Count: 3
Exact Mass: 229.037508
MonoIsotopic Mass: 229.037508
Topological Polar Surface Area: 72.6
Heavy Atom Count: 17
Complexity: 463
Canonical SMILES: C1=CC2=C(C=C1O)OC3=CC(=O)C=CC3=[N+]2[O-]
InChI: InChI=1S/C12H7NO4/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16/h1-6,14H
InChIKey: PLXBWHJQWKZRKG-UHFFFAOYSA-N

Uses

 Resazurin (7-Hydroxy-3H-phenoxazin-3-one-10 oxide (CAS NO.550-82-3)) is a blue dye used mainly as an oxidation-reduction indicator in the resazurin test.

Toxicity Data With Reference

mouse LDLo intravenous 179mg/kg (179mg/kg)   Toxicology and Applied Pharmacology. Vol. 44, Pg. 225, 1978.
 
rat LDLo oral 500mg/kg (500mg/kg)   National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. Vol. 5, Pg. 24, 1953.

Consensus Reports

Reported in EPA TSCA Inventory.

Safety Profile

Moderately toxic by ingestion. When heated to decomposition it emits toxic fumes of NOx.

Specification

 7-Hydroxy-3H-phenoxazin-3-one-10 oxide (CAS NO.550-82-3) is also called Resazurin ; Resazurin tablets ; 3H-Phenoxazin-3-one,7-hydroxy-,10-oxide ; 7-Hydroxy-3H-fenoxazin-3-on-10-oxid ; 7-Hydroxy-3H-phenoxazin-3-on10-oxide ; Azoresorcin ; 7-Hydroxy-10-oxy-phenoxazin-3-one .