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| CAS No.: | 81295-22-9 |
|---|---|
| Name: | HIND III |
| Molecular Structure: | |
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| Formula: | N/A |
| Molecular Weight: | 0 |
| Synonyms: | E.C.3.1.23.21; Endodeoxyribonuclease BbrI; Endodeoxyribonuclease ChuI; EndodeoxyribonucleaseHinbIII; Endodeoxyribonuclease HindIII; Endodeoxyribonuclease HinfII;Endodeoxyribonuclease HsuI; Endodeoxyribonuclease SsbI; HindIII; HindIIIEndonuclease; HindIII restrictase; HindIII restriction endonuclease;Restriction endodeoxyribonuclease HindIII; Restriction endodeoxyribonucleaseHindIII; Restriction endonuclease BspKT8; Restriction endonuclease BspLAIII;Restriction endonuclease BstFI; Restriction endonuclease BstZ1II; Restrictionendonuclease Csp231I; Restriction endonuclease EcoVIII; Restrictionendonuclease HindIII; Restriction endonuclease SpaIV; Restriction enzymeHindIII |
| EINECS: | 279-728-8 |
| Solubility: | very soluble in water |
| Appearance: | Colourless Lipuid |
| Safety: | Mutation data reported. When heated to decomposition it emits acrid smoke and irritating vapors. |
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Chemistry
Product Name: HIND III
Synonyms: Restriction endonucleases HIND III ; HIND III ; HIND III Restriction enzyme ; e.c.3.1.23.21 ; Endodeoxyribonucleasebbri ; Endodeoxyribonucleasechui ; Endodeoxyribonucleasehinbiii ; Endodeoxyribonucleasehindiii
CAS NO: 81295-22-9
EINECS: 279-728-8
Product Categories: 3.1.x.x Acting on esters;3.x.x.x Hydrolases;Molecular Biology;Molecular Biology Enzymes;Molecular Biology Tested;Restriction EndonucleasesApplication Index;Restriction EnzymesEnzyme Class Index
Mol File: Mol File
Storage temp: Store HIND III (CAS NO. 81295-22-9) at -20°C
Form : HIND III (CAS NO. 81295-22-9) is buffered aqueous glycerol solution.
Uses
HIND III (CAS NO. 81295-22-9) as well as other type II restriction endonucleases are very useful in modern science, particularly in DNA sequencing and mapping. Unlike type I restriction enzymes, type II restriction endonucleases perform very specific cleaving of DNA. Type I restriction enzymes recognize specific sequences, but cleave DNA randomly at sites other than their recognition site whereas type II restriction enzymes cleave only at their specific recognition site. Since their discovery in the early 1970s, type II restriction enzymes have revolutionized the way scientists work with DNA, particularly in genetic engineering and molecular biology.
Major uses of type II restriction enzymes include gene analysis and cloning. They have proven to be ideal modeling systems for the study of protein-nucleic acid interactions, structure-function relationships, and the mechanism of evolution.They make good assays for the study of genetic mutations by their ability to specifically cleave DNA to allow the removal or insertion of DNA. Through the use of restriction enzymes, scientists are able to modify, insert, or remove specific genes, a very powerful tool especially when it comes to modifying an organism's genome.
Toxicity Data With Reference
| 1. | msc-ham-ovr 100 units | MUREAV Mutation Research. 326 (1995),83. |
Safety Profile
Mutation data reported. When heated to decomposition it emits acrid smoke and irritating vapors.
WGK Germany 3
Specification
HIND III (CAS NO. 81295-22-9) is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the palindromic DNA sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.
The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends:
5'-A |A G C T T-3'
3'-T T C G A| A-5'
Restriction endonucleases are used as defense mechanisms in prokaryotic organisms in the restriction modification system. Their primary function is to protect the host genome against invasion by foreign DNA, primarily bacteriophage DNA. There is also evidence that suggests the restriction enzymes may act alongside modification enzymes as selfish elements, or may be involved in genetic recombination and transposition.