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9001-99-4

Basic Information
CAS No.: 9001-99-4
Name: Ribonuclease A
Molecular Structure:
Molecular Structure of 9001-99-4 (Ribonuclease A)
Formula: C9H14N4O3
Molecular Weight: 226.23246
Synonyms: Ribonuclease
EINECS: 232-646-6
Density: N/A
Melting Point: 243 °C (dec.)
Boiling Point: N/A
Flash Point: N/A
Solubility: Soluble in water.
Appearance: White to white with a yellow cast powder
Hazard Symbols: N/A
Risk Codes: N/A
Safety: 22-24/25
Transport Information: N/A
PSA: 0.00000
LogP: 0.00000
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  • Ribonuclease A

  • Casno:

    9001-99-4

    Ribonuclease A

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  • Ribonuclease A

  • Casno:

    9001-99-4

    Ribonuclease A

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Chemistry

Molecular Formula: C9H14N4O3
Molecular Weight: 226.2325
EINECS: 207-332-7
Density: 1.375 g/cm3
Melting Point: 243 °C (dec.)
Flash Point: 350.7 °C
Boiling Point: 656.2 °C at 760 mmHg
Vapour Pressure: 4.08E-18 mmHg at 25 °C 
Enthalpy of Vaporization: 101.53 kJ/mol
Storage temp.: 2-8 °C
Structure of Ribonuclease A (CAS NO.9001-99-4):
                      
IUPAC Name: 2-(3-Aminopropanoylamino)-3-(1H-imidazol-5-yl)propanoic acid
Canonical SMILES: C1=C(NC=N1)CC(C(=O)O)NC(=O)CCN
InChI: InChI=1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChIKey: CQOVPNPJLQNMDC-UHFFFAOYSA-N

History

The importance of bovine pancreatic Ribonuclease A (CAS NO.9001-99-4) was secured when the Armour & Co. (of hot dog fame) purified a kilogram of it, and gave 10 mg samples away free to any interested scientists. The ability to have a single lot of purified enzyme instantly made RNase the model system for protein studies.Ribonuclease-S, which is RNase A that has been treated with subtilisin, was the third protein to have its structure solved, in 1967. Studies of the oxidative folding of RNase A led Chris Anfinsen to enunciate the thermodynamic hypothesis of protein folding, which states that the folded form of a protein represents the minimum of its free energy.

Uses

 Ribonuclease A (CAS NO.9001-99-4) is used for biochemical research.

Production

 Ribonuclease A (CAS NO.9001-99-4) can be obtained from the fermentation broth of the grate produced by erythromycin.

Toxicity Data With Reference

Organism Test Type Route Reported Dose (Normalized Dose) Effect Source
mouse LD50 intramuscular 355mg/kg (355mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
mouse LD50 intraperitoneal 250mg/kg (250mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
mouse LD50 subcutaneous 230mg/kg (230mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
rat LD50 intramuscular 310mg/kg (310mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
rat LD50 intraperitoneal 392mg/kg (392mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.
rat LD50 subcutaneous 290mg/kg (290mg/kg)   "Nukleazy: Biologicheskaya Rol i Prakticheskoe Ispol'zovanie. Nucleases: Biological Function and Practical Applications," Berdyshev, G.D., et al., eds., Kiev, USSR, Izdatel'stvo Naukova Dumka, 1985Vol. -, Pg. 56, 1985.

Safety Profile

Safety Statements:
S22:Do not breathe dust. 
S24/25:Avoid contact with skin and eyes.
WGK Germany: 3
RTECS: RF0760000
F 10: Keep under argon.
F 21: Sensitive to humidity.

Specification

 Ribonuclease A , its cas register number is 9001-99-4. It also can be called Alkaline ribonuclease ; Amphinase ; Pancreatic ribonuclease ; RNase; Ribonuclease ; S-RNase ; and Ribonucleic phosphatase .