146998-31-4Relevant articles and documents
Fluorogenic Bifunctional trans-Cyclooctenes as Efficient Tools for Investigating Click-to-Release Kinetics
de Geus, Mark A. R.,Maurits, Elmer,Sarris, Alexi J. C.,Hansen, Thomas,Kloet, Max S.,Kamphorst, Kiki,ten Hoeve, Wolter,Robillard, Marc S.,Pannwitz, Andrea,Bonnet, Sylvestre A.,Codée, Jeroen D. C.,Filippov, Dmitri V.,Overkleeft, Herman S.,van Kasteren, Sander I.
supporting information, p. 9900 - 9904 (2020/06/05)
The inverse electron demand Diels–Alder pyridazine elimination reaction between tetrazines and allylic substituted trans-cyclooctenes (TCOs) is a key player in bioorthogonal bond cleavage reactions. Determining the rate of elimination of alkylamine substrates has so far proven difficult. Here, we report a fluorogenic tool consisting of a TCO-linked EDANS fluorophore and a DABCYL quencher for accurate determination of both the click and release rate constants for any tetrazine at physiologically relevant concentrations.
TUNABLE FLUORESCENCE USING CLEAVABLE LINKERS
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Paragraph 0070-0071, (2014/11/11)
The invention relates to cleavable chemistry in general, and in particular, to tunable fluoresence using cleavable linkers present in fluorochrome-quencher conjugates.
Synthesis of dye-labeled lysine derivatives
Shen, Zongxuan,Zhang, Yawen,Chen, Yan
, p. 2525 - 2532 (2007/10/03)
The N(ε)-dabcyl-N(α)-(9-fluorenylmethoxy)-carbonyllysine was prepared by reaction of lysine-Cu2+ complex with the N-hydroxysuccinimide (HOSu) activated ester of [4-(4'-dimethylamino)phenylazo]benzoic acid (dabcyl acid) followed by treatment with EDTA and acylation with Fmoc-OSu, and the N(α)tert-butyloxycarbonyl-N(ε)-dabcyllysine was prepared by reaction of N(α)-tertbutyloxycarbonyllysine with dabcyl-OSu.