154-87-0 Usage
Originator
Actimide,Tobishi
Uses
Different sources of media describe the Uses of 154-87-0 differently. You can refer to the following data:
1. Thiamine Pyrophosphate is a thiamine (T344185) derivative produced by enzyme thiamine pyrophosphatase. Thiamine pyrophosphate is a cofactor used to catalyze various biochemical reactions.
2. Cocarboxylase is a thiamine (T344185) derivative produced by enzyme thiamine pyrophosphatase. Thiamine pyrophosphate is a cofactor used to catalyze various biochemical reactions.
3. Cocarboxylase can be used in decarboxylation mechanisms in biological systems,and to investigate radical reactions of thiamin pyrophosphate in 2-oxoacid oxidoreductases.
Definition
The active coenzyme
form of vitamin B1 which functions in aldehyde
transfer reactions.
Manufacturing Process
4.5 kg of aqueous 89% orthophosphoric acid are heated to 135°C, and kept
at this temperature for about 3 h while being actively stirred. Then, the
heating is discontinued and 3.5 kg of phosphorouspentoxide are added during
a period of 2.5 to 3 h, while being actively stirred. During this period, the
interior temperature rises to 165°-175°C. After completion of the addition of
phosphorouspentoxide, the stirring is continued until all phosphorouspentoxide
is dissolved. The phosphoric acid mixture thus produced is subsequently
cooled down to 130°C.
At this temperature 2.0 kg of thiamine chloride hydrochloride (vitamin B1)
were added during 2 to 3 h while being well stirred. The stirring is continued
at 130°C until the phosphorylated mixture no longer contains chlorine ions. A
phosphorylated melt is thus obtained. The thus obtained phosphorylation melt
is dissolved in 6-8 L of water (with ice) at a temperature below 10°C, while
being vigorously stirred. The aqueous solution is stirred into 100 L of 96%
alcohol and left standing overnight. The supernatant solvent is decanted from
the separated syrup; the latter is taken up in 4 L of water. The solution thus
obtained is fed, depending upon the volume of phosphoric acid contained
therein, to an exchanger column filled with anion exchanger (weak basic, for
instance Amberlite IRA 45, 20-30 L) (a polystyrene resin with primary,
secondary and quaternary amino groups). The solution is caused to seep into
the column from the top thereof and is then washed with water until the runoff at the bottom no longer shows any thiamine reaction. About 25 L of the
solution are obtained, which are concentrated to 6 L at 30°C and 12 Torr. The
concentrated residue is added to 20 to 30 L of a cationic exchanger (Amberlite
IRC 50) in order to separate the thiamine-orthophosphoric acid ester from the
thiamine-pyrophosphoric acid ester, and subsequently washed with water until
the eluate is free of thiamine.
70-80 L of a solution are obtained which are concentrated to 1.5 L in a
circulation evaporator at 30°C and 12 Torr. 7.5 L of 96% ethanol are slowly
added to a concentrate while being stirred. The cocarboxylase-tetrahydrate
separates in the form of fine needles. The yield is 530.0 g with a melting point
of 220°-225°C (dec.).
10.0 g of cocarboxylase-tetrahydrate are dissolved in 25 ml of 5% aqueous
hydrochloric acid, and 75 ml acetone are added dropwise while being stirred.
The precipitated hydrochloride of the cocarboxylase, melting point 240°C is
sucked off. The yield is 9.5 g.
Therapeutic Function
Coenzyme, Metabolic
General Description
Certified pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards. Thiamine pyrophosphate is a coenzyme of transketolase that catalyzes the cleavage of ribulose-5-phosphate; thereby forming D-glyceraldehyde-3-phosphate. This reaction requires the addition of an acceptor aldehyde such as ribose-5-phosphate, glyceraldehye or glycolaldehyde.
Biochem/physiol Actions
Thiamine pyrophosphate (TPP) is a coenzyme of transketolase, that catalyzes the cleavage of ribulose-5-phosphate. This reaction leads to the formation of D-glyceraldehyde-3-phosphate. It requires the addition of an acceptor aldehyde such as ribose-5-phosphate, glyceraldehye or glycolaldehyde. TPP is capable of decarboxylating hydroxypyruvate in the presence of an ′acceptor aldehyde′.
Check Digit Verification of cas no
The CAS Registry Mumber 154-87-0 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 1,5 and 4 respectively; the second part has 2 digits, 8 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 154-87:
(5*1)+(4*5)+(3*4)+(2*8)+(1*7)=60
60 % 10 = 0
So 154-87-0 is a valid CAS Registry Number.
InChI:InChI=1/C12H18N4O7P2S.ClH/c1-8-11(3-4-22-25(20,21)23-24(17,18)19)26-7-16(8)6-10-5-14-9(2)15-12(10)13;/h5,7H,3-4,6H2,1-2H3,(H4-,13,14,15,17,18,19,20,21);1H
154-87-0Relevant articles and documents
Synthesis and characterization of catalytically active thiazolium gold(i)-carbenes
Dince, Clément C.,Meoded, Roy A.,Hilvert, Donald
, p. 7585 - 7587 (2017)
Thiamin analogs were used to synthesize mono gold(i)-carbene derivatives in a single step under aqueous conditions. The resulting thiazolium gold(i)-carbenes catalyze 5-endo-dig carbocyclization of an acetylenic dicarbonyl compound in organic solvents and hydroalkoxylation of an allene in aqueous buffer.
Preparation method of co-carboxylase and tetrahydrate or salt thereof
-
Paragraph 0043; 0049-0052; 0056-0058; 0062-0075, (2021/09/21)
The invention discloses a preparation method of co-carboxylase and tetrahydrate or salt thereof. The invention provides a preparation method of a co-carboxylase shown as a formula I. The method comprises the following steps: eluting a mixture containing the co-carboxylase as shown in the formula I by using weakly-alkaline anion exchange resin through water and a weakly-alkaline aqueous solution with the pH value of 10-12 as eluent in sequence, and collecting eluate to obtain an aqueous solution containing the co-carboxylase as shown in the formula I, wherein the mixture containing the co-carboxylase as shown in the formula I comprises thiamine monophosphate and the co-carboxylase as shown in the formula I.
Use of vitamin b1 as agents for controlling plant diseases
-
, (2008/06/13)
The present invention relates to an agent for controlling plant diseases containing vitamin B1, or salts or derivatives thereof as an active ingredient, which exhibits excellent disease controlling effects by rapid induction of defense-related genes in plants infected with pathogens.
