Z-Leu-Gly-ONp, also known as N-benzyloxycarbonyl-leucyl-glycine p-nitrophenyl ester, is a synthetic tripeptide derivative commonly used in biochemical research and assays. It consists of three amino acids: leucine (Leu), glycine (Gly), and a p-nitrophenyl (ONp) ester group. The "Z" prefix indicates the presence of a benzyloxycarbonyl (Z) protecting group, which is used to temporarily block the amino group of the leucine residue, preventing unwanted side reactions. Z-Leu-Gly-ONp is often employed as a substrate for protease enzymes, allowing researchers to study enzyme kinetics, specificity, and inhibition. Upon enzymatic cleavage, the p-nitrophenyl group is released, and its absorbance can be measured, providing a quantitative assessment of enzyme activity.
The CAS Registry Mumber 10065-66-4 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,0,6 and 5 respectively; the second part has 2 digits, 6 and 6 respectively. Calculate Digit Verification of CAS Registry Number 10065-66: (7*1)+(6*0)+(5*0)+(4*6)+(3*5)+(2*6)+(1*6)=64 64 % 10 = 4 So 10065-66-4 is a valid CAS Registry Number.